|
|
|
|||||||
|
|||||||||
(Received for publication, January 19, 1996, and in revised form, April 16, 1996)
From the The rat Calbindin-D9K (CaBP9K) gene is mainly
expressed in intestine, uterus, and lung and is regulated in a complex
tissue-specific manner. To analyze the role of potential regulatory
elements, previously defined by DNaseI hypersensivity, we made
transgenic mice containing truncated rat CaBP9K fusion gene with simian
virus 40 large T antigen and the chloramphenicol acetyltransferase as
reporter genes. The transgenes contained CaBP9K promoter fragments with
5
Volume 271, Number 28,
Issue of July 12, 1996
pp. 16820-16826
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
,
,
,
and
Institut Cochin de Génétique
Moléculaire, INSERM U129, Université Réné
Descartes, 24 rue du Faubourg Saint-Jacques, 75014 Paris, the
§ INSERM U246, Faculté de Médecine Xavier
Bichat, Institut Fédératif de recherche, B.P. 416, 75780 Paris Cedex 18, the ¶ INSERM U120, Hôpital Robert
Debré, 48 boulevard Sérurier, 75019 Paris, and the
Laboratoire Universitaire de Recherche en Histopathologie, 15 rue de l'Ecole de Médecine, 75006 Paris, France
end points at 
4400, 1011, and 117 base pairs (bp), whereas the
3 end points was at +365 bp. Northern blot analysis of T antigen
expression and chloramphenicol acetyltransferase enzyme-linked
immunosorbent assay indicated that a positive element, probably the
distal intestine-specific DNaseI HS, necessary to target the expression
of the transgene in the intestine, is present between 4400 and 1011
bp. The cephalo-caudal gradient of expression of the transgene along
the small intestine was similar to those of the endogenous gene, but an
ectopic expression of the transgene was observed in the colon. The
1011 transgene was expressed in epithelial alveolar cells of the
lung, in renal proximal tubule cells, and in uterine myometrium, as
judged from immunocytochemical, histological, and Northern blot
analyses. The shortest, 117 construct was only expressed in uterine
myometrium, and it was under a strict estrogen dependence like the
endogenous gene. Finally, responsiveness to vitamin D in the duodenum
was observed with the largest, 4400 construct. Thus, different
tissues utilize distinct cis-acting elements to direct and regulate the
expression of the rat CaBP9K gene.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Song, S. Kato, and J. C. Fleet Vitamin D Receptor (VDR) Knockout Mice Reveal VDR-Independent Regulation of Intestinal Calcium Absorption and ECaC2 and Calbindin D9k mRNA J. Nutr., February 1, 2003; 133(2): 374 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Lee, Z. Wang, C.-K. Lin, C. H. Contag, L. C. Olds, A. D. Cooper, and E. Sibley Regulation of Intestine-specific Spatiotemporal Expression by the Rat Lactase Promoter J. Biol. Chem., April 5, 2002; 277(15): 13099 - 13105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cadoret, C. Ovejero, S. Saadi-Kheddouci, E. Souil, M. Fabre, B. Romagnolo, A. Kahn, and C. Perret Hepatomegaly in Transgenic Mice Expressing an Oncogenic Form of {beta}-Catenin Cancer Res., April 1, 2001; 61(8): 3245 - 3249. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. R. Dusing, A. G. Brickner, S. Y. Lowe, M. B. Cohen, and D. A. Wiginton A duodenum-specific enhancer regulates expression along three axes in the small intestine Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G1080 - G1093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Colnot, C. Ovejero, B. Romagnolo, A. Porteu, P. Lacourte, M. Thomasset, and C. Perret Transgenic Analysis of the Response of the Rat Calbindin-D 9k Gene to Vitamin D Endocrinology, July 1, 2000; 141(7): 2301 - 2308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Romagnolo, D. Berrebi, S. Saadi-Keddoucci, A. Porteu, A.-l. Pichard, M. Peuchmaur, A. Vandewalle, A. Kahn, and C. Perret Intestinal Dysplasia and Adenoma in Transgenic Mice after Overexpression of an Activated {beta}-Catenin Cancer Res., August 1, 1999; 59(16): 3875 - 3879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tatsumi, T. Higuchi, H. Fujiwara, T. Nakayama, K. Itoh, T. Mori, S. Fujii, and J. Fujita Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantation Mol. Hum. Reprod., February 1, 1999; 5(2): 153 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Colnot, B. Romagnolo, M. Lambert, F. Cluzeaud, A. Porteu, A. Vandewalle, M. Thomasset, A. Kahn, and C. Perret Intestinal Expression of the Calbindin-D9K Gene in Transgenic Mice. REQUIREMENT FOR A Cdx2-BINDING SITE IN A DISTAL ACTIVATOR REGION J. Biol. Chem., November 27, 1998; 273(48): 31939 - 31946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Raval-Pandya, L. P. Freedman, H. Li, and S. Christakos Thyroid Hormone Receptor Does Not Heterodimerize with the Vitamin D Receptor but Represses Vitamin D Receptor-Mediated Transactivation Mol. Endocrinol., September 1, 1998; 12(9): 1367 - 1379. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Perez and D. B. Kelley Androgen Mitigates Axotomy-Induced Decreases in Calbindin Expression in Motor Neurons J. Neurosci., October 1, 1997; 17(19): 7396 - 7403. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |