|
|
|
|||||||
|
|||||||||
(Received for publication, February 29, 1996, and in revised form, April 14, 1996)
From the The enzyme hepatic lipase may play several roles
in lipoprotein metabolism. Recent investigation has suggested a role
for the enzyme in lipoprotein and/or lipoprotein lipid uptake. To study
this, a simple isolated system that mimics the in vivo
system would be desirable. The enzyme is secreted by the hepatic
parenchymal cell but exists, and presumably exerts its effects, while
bound to capillary endothelial cells in the liver, adrenal gland, and
the ovary. We constructed a cDNA that encodes the expression of a
chimeric protein composed of rat hepatic lipase and the signal sequence
for the addition of the glycophosphatidylinositol (GPI) anchor from
human decay-accelerating factor. When transfected into Chinese hamster
ovary (CHO) cells this gave rise to a cell population that had
immunoreactive hepatic lipase on the cell surface. Cloning of the
transfected cells produced several cell lines that expressed the
chimeric protein bound to the cell surface by a GPI anchor. This was
documented by demonstrating incorporation of
[3H]ethanolamine into anti-hepatic lipase
immunoprecipitable material; in addition, hepatic lipase was released
from the cells by phosphatidylinositol-specific phospholipase C but not
by heparin. Phosphatidylinositol-phospholipase C treatment of cells
expressing the anchored lipase released material that comigrated with
hepatic lipase on SDS-polyacrylamide gel electrophoresis and was
immunoreactive with antibody to the cross-reacting determinant of GPI
anchors. Cell lysates containing the anchored protein contained
salt-resistant lipase activity, a known feature of the secreted hepatic
lipase; thus it appears that these cells have a surface-anchored
hepatic lipase molecule. Although it was not possible to demonstrate
lipolysis by the enzyme while it was on the cell surface for technical
reasons, the protein produced by these cells was active when studied in
cell membranes. The ability of the cells to take up lipoproteins was
studied. The cells demonstrated an increased affinity for low density
lipoprotein (LDL) receptor mediated uptake of LDL. They did not,
however, demonstrate any enhanced binding or removal of chylomicron
remnants. With respect to LDL and remnants, the cells expressing
anchored lipase behaved similarly to CHO cell that expressed secreted
hepatic lipase. The cells expressing anchored hepatic lipase had a
marked increase in the uptake of high density lipoprotein and high
density lipoprotein cholesteryl ester when compared to that seen with
CHO cells secreting hepatic lipase. This increase occurred primarily
via the selective pathway, and was not reduced by addition of anti-LDL
receptor or anti-hepatic lipase antibodies or the receptor-associated
protein. Together the results suggest that hepatic lipase, when bound
to the cell surface by a GPI anchor, plays a role in enhancing
lipoprotein uptake. For LDL this may involve the provision of a second
foot for particle binding, thus enhancing affinity for the LDL
receptor. For chylomicron remnants an additional molecule or molecules
are necessary to mediate this effect. For HDL, the enzyme facilitates
uptake of cholesteryl ester primarily by the selective pathway.
Volume 271, Number 28,
Issue of July 12, 1996
pp. 16906-16914
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
CHARACTERIZATION OF LOW DENSITY LIPOPROTEIN AND CHYLOMICRON
REMNANT UPTAKE AND SELECTIVE UPTAKE OF HIGH DENSITY
LIPOPROTEIN-CHOLESTERYL ESTER
,¶
Research Institute, Palo Alto Medical
Foundation, ¶ Department of Medicine, Stanford University and
§ Geriatric Research, Education and Clinical Center,
Veterans Administration Palo Alto Health Care System,
Palo Alto, California 94301
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Freeman, M. J. A. Amar, R. Shamburek, B. Paigen, H. B. Brewer Jr., S. Santamarina-Fojo, and H. Gonzalez-Navarro Lipolytic and ligand-binding functions of hepatic lipase protect against atherosclerosis in LDL receptor-deficient mice J. Lipid Res., January 1, 2007; 48(1): 104 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Dichek, N. Agrawal, N. E. Andaloussi, and K. Qian Attenuated corticosterone response to chronic ACTH stimulation in hepatic lipase-deficient mice: evidence for a role for hepatic lipase in adrenal physiology Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E908 - E915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Gonzalez-Navarro, Z. Nong, M. J. A. Amar, R. D. Shamburek, J. Najib-Fruchart, B. J. Paigen, H. B. Brewer Jr., and S. Santamarina-Fojo The Ligand-binding Function of Hepatic Lipase Modulates the Development of Atherosclerosis in Transgenic Mice J. Biol. Chem., October 29, 2004; 279(44): 45312 - 45321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Santamarina-Fojo, H. Gonzalez-Navarro, L. Freeman, E. Wagner, and Z. Nong Hepatic Lipase, Lipoprotein Metabolism, and Atherogenesis Arterioscler. Thromb. Vasc. Biol., October 1, 2004; 24(10): 1750 - 1754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-J. Lee, S. Kadambi, C. David, A. D. Cooper, and S. Y. Choi Association of hepatic lipase with proteoglycans stimulates the production of proteoglycans in vivo and in vitro J. Lipid Res., July 1, 2004; 45(7): 1266 - 1271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. V. Fuki, N. Blanchard, W. Jin, D. H. L. Marchadier, J. S. Millar, J. M. Glick, and D. J. Rader Endogenously Produced Endothelial Lipase Enhances Binding and Cellular Processing of Plasma Lipoproteins via Heparan Sulfate Proteoglycan-mediated Pathway J. Biol. Chem., September 5, 2003; 278(36): 34331 - 34338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rigotti, H. E. Miettinen, and M. Krieger The Role of the High-Density Lipoprotein Receptor SR-BI in the Lipid Metabolism of Endocrine and Other Tissues Endocr. Rev., June 1, 2003; 24(3): 357 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Ramsamy, J. Boucher, R. J. Brown, Z. Yao, and D. L. Sparks HDL regulates the displacement of hepatic lipase from cell surface proteoglycans and the hydrolysis of VLDL triacylglycerol J. Lipid Res., April 1, 2003; 44(4): 733 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Choi, K.-i. Hirata, T. Ishida, T. Quertermous, and A. D. Cooper Endothelial lipase: a new lipase on the block J. Lipid Res., November 1, 2002; 43(11): 1763 - 1769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mardones, V. Quiñones, L. Amigo, M. Moreno, J. F. Miquel, M. Schwarz, H. E. Miettinen, B. Trigatti, M. Krieger, S. VanPatten, et al. Hepatic cholesterol and bile acid metabolism and intestinal cholesterol absorption in scavenger receptor class B type I-deficient mice J. Lipid Res., February 1, 2001; 42(2): 170 - 180. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. Medh, G. L. Fry, S. L. Bowen, S. Ruben, H. Wong, and D. A. Chappell Lipoprotein lipase- and hepatic triglyceride lipase- promoted very low density lipoprotein degradation proceeds via an apolipoprotein E-dependent mechanism J. Lipid Res., November 1, 2000; 41(11): 1858 - 1871. [Abstract] [Full Text]
|
|
|
|
|
|
G. Lambert, M. J. A. Amar, P. Martin, J. Fruchart-Najib, B. Föger, R. D. Shamburek, H. B. Brewer , Jr., and S. Santamarina-Fojo Hepatic lipase deficiency decreases the selective uptake of HDL-cholesteryl esters in vivo J. Lipid Res., May 1, 2000; 41(5): 667 - 672. [Abstract] [Full Text]
|
|
|
|
|
|
K. A. Dugi, M. J. A. Amar, C. C. Haudenschild, R. D. Shamburek, A. Bensadoun, R. F. Hoyt Jr, J. Fruchart-Najib, Z. Madj, H. B. Brewer Jr, and S. Santamarina-Fojo In Vivo Evidence for Both Lipolytic and Nonlipolytic Function of Hepatic Lipase in the Metabolism of HDL Arterioscler. Thromb. Vasc. Biol., March 1, 2000; 20(3): 793 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Carr, J. E. Hokanson, S. S. Deeb, J. Q. Purnell, E. S. Mitchell, and J. D. Brunzell A Hepatic Lipase Gene Promoter Polymorphism Attenuates the Increase in Hepatic Lipase Activity With Increasing Intra-abdominal Fat in Women Arterioscler. Thromb. Vasc. Biol., November 1, 1999; 19(11): 2701 - 2707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Collet, A. R. Tall, H. Serajuddin, K. Guendouzi, L. Royer, H. Oliveira, R. Barbaras, X.-c. Jiang, and O. L. Francone Remodeling of HDL by CETP in vivo and by CETP and hepatic lipase in vitro results in enhanced uptake of HDL CE by cells expressing scavenger receptor B-I J. Lipid Res., July 1, 1999; 40(7): 1185 - 1193. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. Medh, S. L. Bowen, G. L. Fry, S. Ruben, J. Hill, H. Wong, and D. A. Chappell Hepatic triglyceride lipase promotes low density lipoprotein receptor-mediated catabolism of very low density lipoproteins in vitro J. Lipid Res., July 1, 1999; 40(7): 1263 - 1275. [Abstract] [Full Text]
|
|
|
|
|
|
R. W. Mahley and Z.-S. Ji Remnant lipoprotein metabolism: key pathways involving cell-surface heparan sulfate proteoglycans and apolipoprotein E J. Lipid Res., January 1, 1999; 40(1): 1 - 16. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. A. Amar, K. A. Dugi, C. C. Haudenschild, R. D. Shamburek, B. Foger, M. Chase, A. Bensadoun, R. F. Hoyt , Jr., H. B. Brewer , Jr., and S. Santamarina-Fojo Hepatic lipase facilitates the selective uptake of cholesteryl esters from remnant lipoproteins in apoE-deficient mice J. Lipid Res., December 1, 1998; 39(12): 2436 - 2442. [Abstract] [Full Text]
|
|
|
|
|
|
X. Gu, B. Trigatti, S. Xu, S. Acton, J. Babitt, and M. Krieger The Efficient Cellular Uptake of High Density Lipoprotein Lipids via Scavenger Receptor Class B Type I Requires Not Only Receptor-mediated Surface Binding but Also Receptor-specific Lipid Transfer Mediated by Its Extracellular Domain J. Biol. Chem., October 9, 1998; 273(41): 26338 - 26348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Donner, S. Choi, M. Komaromy, and A. D. Cooper Accelerated lipoprotein uptake by transplantable hepatomas that express hepatic lipase J. Lipid Res., September 1, 1998; 39(9): 1805 - 1815. [Abstract] [Full Text]
|
|
|
|
|
|
S. Y. Choi, I. J. Goldberg, L. K. Curtiss, and A. D. Cooper Interaction between ApoB and Hepatic Lipase Mediates the Uptake of ApoB-containing Lipoproteins J. Biol. Chem., August 7, 1998; 273(32): 20456 - 20462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Azhar, A. Nomoto, S. Leers-Sucheta, and E. Reaven Simultaneous induction of an HDL receptor protein (SR-BI) and the selective uptake of HDL-cholesteryl esters in a physiologically relevant steroidogenic cell model J. Lipid Res., August 1, 1998; 39(8): 1616 - 1628. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. L. Varban, F. Rinninger, N. Wang, V. Fairchild-Huntress, J. H. Dunmore, Q. Fang, M. L. Gosselin, K. L. Dixon, J. D. Deeds, S. L. Acton, et al. Targeted mutation reveals a central role for SR-BI in hepatic selective uptake of high density lipoprotein cholesterol PNAS, April 14, 1998; 95(8): 4619 - 4624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. Martins and T. G. Redgrave A 13CO2 breath test to assess the metabolism of triglyceride-rich lipoprotein remnants in mice J. Lipid Res., March 1, 1998; 39(3): 691 - 698. [Abstract] [Full Text]
|
|
|
|
|
|
H. L. Dichek, W. Brecht, J. Fan, Z.-S. Ji, S. P. A. McCormick, H. Akeefe, L. Conzo, D. A. Sanan, K. H. Weisgraber, S. G. Young, et al. Overexpression of Hepatic Lipase in Transgenic Mice Decreases Apolipoprotein B-containing and High Density Lipoproteins. EVIDENCE THAT HEPATIC LIPASE ACTS AS A LIGAND FOR LIPOPROTEIN UPTAKE J. Biol. Chem., January 23, 1998; 273(4): 1896 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-S. Ji, H. L. Dichek, R. D. Miranda, and R. W. Mahley Heparan Sulfate Proteoglycans Participate in Hepatic Lipaseand Apolipoprotein E-mediated Binding and Uptake of Plasma Lipoproteins, Including High Density Lipoproteins J. Biol. Chem., December 12, 1997; 272(50): 31285 - 31292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Ramsamy, T. A.-M. Neville, B. M. Chauhan, D. Aggarwal, and D. L. Sparks Apolipoprotein A-I Regulates Lipid Hydrolysis by Hepatic Lipase J. Biol. Chem., October 20, 2000; 275(43): 33480 - 33486. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |