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(Received for publication, March 7, 1996, and in revised form, April 18, 1996)
From the URA CNRS 0210 ``Neurobiologie Moléculaire,''
Département des Biotechnologies, Institut Pasteur, 25 rue du Dr
Roux, 75724 Paris Cedex 15, France
The adult neuromuscular junction displays an
accumulation of both the acetylcholine receptor (AChR) protein in the
subneural domain of the post-synaptic membrane and the mRNAs coding
for all its subunits at the level of the subjunctional ``fundamental
nuclei.'' In the course of end plate development, the
Volume 271, Number 29,
Issue of July 19, 1996
pp. 17433-17438
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
-Subunit Gene
-subunit, at
variance with other subunits, becomes exclusively expressed at the
level of the fundamental nuclei, yet at a rather late stage (around
birth). To analyze the promoter region of the -subunit gene which
directs its specific expression at the synapse, we used a quantitative
transient in vivo expression assay in intact muscle tissue
using constructs of the -subunit promoter placed upstream of the
-galactosidase reporter gene. One crucial element for
synapse-specific expression was detected between the 
11 and 6
positions. Disruption of this element, either by a scanning mutation or
single base mutations, greatly diminishes, or even completely inhibits,
preferential expression of the transgene at the end plate. Gel shift
experiments reveal the presence of a complex in nuclear muscle extracts
that bind the core sequence of this element. The identification of such
a site opens the possibility to identify regulatory factors responsible
for compartmentalized expression at the neuromuscular junction.
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