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(Received for publication, March 22, 1996)
From the Department of Immunology, University of Texas, M.D.
Anderson Cancer Center, Houston, Texas 77030 and the
The Pem gene encodes an atypical
homeodomain protein, distantly related to Prd/Pax family
members, that we demonstrate is regulated in a complex transcriptional
and post-transcriptional manner. We show that the rat Pem
genomic structure includes three 5
Volume 271, Number 29,
Issue of July 19, 1996
pp. 17536-17546
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
ANDROGEN-DEPENDENT AND -INDEPENDENT PROMOTERS AND
TISSUE-SPECIFIC ALTERNATIVE RNA SPLICING
,
and
Molecular Microbiology and Immunology Department, Oregon
Health Sciences University, Portland, Oregon 97201
-untranslated (5-UT) exons
and four coding exons, three of which encode the homeodomain. Several
alternatively spliced transcripts were identified, including one that
skips an internal coding exon, enabling this mRNA to express a
novel form of the Pem protein. Other alternatively spliced mRNAs
were characterized that possess different 5-UT regions, including a
muscle-specific transcript. The different 5-UT termini present in
Pem transcripts conferred different levels of
translatability in vitro. Two promoters containing multiple
transcription initiation sites were identified: a distal promoter
(Pd) in the first 5-UT exon and a proximal promoter
(Pp) located in the ``intron'' upstream of the first
coding exon. The Pd was active in placenta, ovary, tumor
cell lines, and to a lesser extent in skeletal muscle. In contrast,
transcripts from the Pp were only detectable in testis and
epididymis and were only expressed in epididymis in the presence of
testosterone. To our knowledge no transcription factors have previously
been identified that exhibit androgen-dependent expression
in the epididymis.
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