We have examined the effects of illumination, carbon source, and levels of chloroplast protein synthesis on trans-acting proteins that bind to the leaders of five representative chloroplast mRNAs. The accumulation of these five chloroplast mRNAs and the proteins they encode were measured in cells grown under identical conditions. Extracts from all cell types examined contain a minimum set of six chloroplast 5`-untranslated region (UTR)-binding proteins (81, 62, 56, 47, 38, and 15 kDa). Fractionation results suggest that multiple forms of the 81-, 62-, and 47-kDa proteins may exist. A 36-kDa protein was found in all cells except those deficient in chloroplast protein synthesis. Binding of the 81-, 47-, and 38-kDa proteins to the rps12 leader is effectively competed by the atpB or rbcL 5`-UTRs, indicating that the same proteins bind to all three leaders. In contrast, these three proteins do not bind to the nuclear-encoded -1 tubulin leader, which bound novel proteins of 110, 70, and 43 kDa. Cis-acting sequences within the 5`-UTRs of two chloroplast mRNAs (rps7 and atpB) have been identified which are protected from digestion by RNase T1 by extracts enriched for the 81-, 47-, and 38-kDa proteins.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. Glanz, A. Bunse, A. Wimbert, C. Balczun, and U. Kuck A nucleosome assembly protein-like polypeptide binds to chloroplast group II intron RNA in Chlamydomonas reinhardtii Nucleic Acids Res., October 6, 2006; 34(18): 5337 - 5351. [Abstract] [Full Text] [PDF]

				I. Yosef, V. Irihimovitch, J. A. Knopf, I. Cohen, I. Orr-Dahan, E. Nahum, C. Keasar, and M. Shapira RNA Binding Activity of the Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit from Chlamydomonas reinhardtii J. Biol. Chem., March 12, 2004; 279(11): 10148 - 10156. [Abstract] [Full Text] [PDF]

				F. Ossenbühl and J. Nickelsen cis- and trans-Acting Determinants for Translation of psbD mRNA in Chlamydomonas reinhardtii Mol. Cell. Biol., November 1, 2000; 20(21): 8134 - 8142. [Abstract] [Full Text]

				D. C. Higgs, R. S. Shapiro, K. L. Kindle, and D. B. Stern Small cis-Acting Sequences That Specify Secondary Structures in a Chloroplast mRNA Are Essential for RNA Stability and Translation Mol. Cell. Biol., December 1, 1999; 19(12): 8479 - 8491. [Abstract] [Full Text] [PDF]

				D. C. Fargo, J. E. Boynton, and N. W. Gillham Mutations Altering the Predicted Secondary Structure of a Chloroplast 5' Untranslated Region Affect Its Physical and Biochemical Properties as Well as Its Ability To Promote Translation of Reporter mRNAs Both in the Chlamydomonas reinhardtii Chloroplast and in Escherichia coli Mol. Cell. Biol., October 1, 1999; 19(10): 6980 - 6990. [Abstract] [Full Text] [PDF]

				D. J. McCormac and A. Barkan A Nuclear Gene in Maize Required for the Translation of the Chloroplast atpB/E mRNA PLANT CELL, September 1, 1999; 11(9): 1709 - 1716. [Abstract] [Full Text]

				M. Markus and R. Benezra Two Isoforms of Protein Disulfide Isomerase Alter the Dimerization Status of E2A Proteins by a Redox Mechanism J. Biol. Chem., January 8, 1999; 274(2): 1040 - 1049. [Abstract] [Full Text] [PDF]

				S. K. Muhlbauer and L. A. Eichacker Light-dependent Formation of the Photosynthetic Proton Gradient Regulates Translation Elongation in Chloroplasts J. Biol. Chem., August 14, 1998; 273(33): 20935 - 20940. [Abstract] [Full Text] [PDF]

				N.-J. Yu and L. L. Spremulli Regulation of the Activity of Chloroplast Translational Initiation Factor 3 by NH2- and COOH-Terminal Extensions J. Biol. Chem., February 13, 1998; 273(7): 3871 - 3877. [Abstract] [Full Text] [PDF]

				W. Zerges and J.-D. Rochaix Low Density Membranes Are Associated with RNA-binding Proteins and Thylakoids in the Chloroplast of Chlamydomonas reinhardtii J. Cell Biol., January 12, 1998; 140(1): 101 - 110. [Abstract] [Full Text] [PDF]

				J. Kim and S. P. Mayfield Protein Disulfide Isomerase as a Regulator of Chloroplast Translational Activation Science, December 12, 1997; 278(5345): 1954 - 1957. [Abstract] [Full Text]

				D. J. McCormac, H. Litz, J. Wang, P. D. Gollnick, and J. O. Berry Light-associated and Processing-dependent Protein Binding to 5' Regions of rbcL mRNA in the Chloroplasts of a C4 Plant J. Biol. Chem., January 26, 2001; 276(5): 3476 - 3483. [Abstract] [Full Text] [PDF]