The pur cluster which encodes the puromycin biosynthetic pathway from Streptomyces alboniger was subcloned as a 13-kilobase fragment in plasmid pIJ702 and expressed in an apparently regulated manner in the heterologous host Streptomyces lividans. The sequencing of a 9.1-kilobase DNA fragment completed the sequence of pur. This permitted identification of seven new open reading frames in the order: napH, pur7, pur10, pur6, pur4, pur5, and pur3. The latter is followed by the known pac, dmpM, and pur8 genes. Nine open reading frames are transcribed rightward as a unit in opposite direction to that of the pur8 gene which is expressed as a monocistronic transcript from the rightmost end. napH encodes the known N-acetylpuromycin N-acetylhydrolase. The deduced products from other open reading frames present similarities to: NTP pyrophosphohydrolases (pur7), several oxidoreductases (pur10), the putative LmbC protein of the lincomycin biosynthetic pathway from Streptomyces lincolnensis (pur6), S-adenosylmethionine-dependent methyltransferases (pur5), a variety of presumed aminotransferases (pur4), and several monophosphatases (pur3). According to these similarities and to previous biochemical work, a puromycin biosynthetic pathway has been deduced. No cluster-associated regulatory gene was found. However, both pur10 and pur6 genes contain a TTA codon, which suggests that they are translationally controlled by the bldA gene product, a specific tRNA.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				E.-S. E. Habib, J. N. Scarsdale, and K. A. Reynolds Biosynthetic Origin of Hygromycin A Antimicrob. Agents Chemother., July 1, 2003; 47(7): 2065 - 2071. [Abstract] [Full Text] [PDF]

				C.-S. Zhang, A. Stratmann, O. Block, R. Bruckner, M. Podeschwa, H.-J. Altenbach, U. F. Wehmeier, and W. Piepersberg Biosynthesis of the C7-cyclitol Moiety of Acarbose in Actinoplanes Species SE50/110. 7-O-PHOSPHORYLATION OF THE INITIAL CYCLITOL PRECURSOR LEADS TO PROPOSAL OF A NEW BIOSYNTHETIC PATHWAY J. Biol. Chem., June 14, 2002; 277(25): 22853 - 22862. [Abstract] [Full Text] [PDF]

				R. H. Ffrench-Constant, N. Waterfield, V. Burland, N. T. Perna, P. J. Daborn, D. Bowen, and F. R. Blattner A Genomic Sample Sequence of the Entomopathogenic Bacterium Photorhabdus luminescens W14: Potential Implications for Virulence Appl. Envir. Microbiol., August 1, 2000; 66(8): 3310 - 3329. [Abstract] [Full Text]

				M. J. F. Lozano, L. L. Remsing, L. M. Quiros, A. F. Brana, E. Fernandez, C. Sanchez, C. Mendez, J. Rohr, and J. A. Salas Characterization of Two Polyketide Methyltransferases Involved in the Biosynthesis of the Antitumor Drug Mithramycin by Streptomyces argillaceus J. Biol. Chem., February 4, 2000; 275(5): 3065 - 3074. [Abstract] [Full Text] [PDF]

				J. Gagnat, H. Chouayekh, C. Gerbaud, F. Francou, and M.-J. Virolle Disruption of sblA in Streptomyces lividans permits expression of a heterologous {alpha}-amylase gene in the presence of glucose Microbiology, September 1, 1999; 145(9): 2303 - 2312. [Abstract] [Full Text] [PDF]

				J. C. Espinosa, J. A. Tercero, M. A. Rubio, and A. Jiménez The pur7 Gene from the Puromycin Biosynthetic pur Cluster of Streptomyces alboniger Encodes a Nudix Hydrolase J. Bacteriol., August 15, 1999; 181(16): 4914 - 4918. [Abstract] [Full Text]

				C.-G. Kim, T.-W. Yu, C. B. Fryhle, S. Handa, and H. G. Floss 3-Amino-5-hydroxybenzoic Acid Synthase, the Terminal Enzyme in the Formation of the Precursor of mC7N Units in Rifamycin and Related Antibiotics J. Biol. Chem., March 13, 1998; 273(11): 6030 - 6040. [Abstract] [Full Text] [PDF]

				M. J. Bessman, D. N. Frick, and S. F. O'Handley The MutT Proteins or ``Nudix'' Hydrolases, a Family of Versatile, Widely Distributed, ``Housecleaning'' Enzymes J. Biol. Chem., October 11, 1996; 271(41): 25059 - 25062. [Full Text] [PDF]