| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, May 6, 1996)
,
,
,
From the Insulin stimulation of 3T3-L1 adipocytes causes
rapid translocation of actin and the GLUT4 glucose transporter to the
plasma membrane. Both processes depend on the activity of
phosphatidylinositol 3-kinase. Using single cell microinjection, we
have transiently expressed a constitutively activated mutant of
phosphatidylinositol 3-kinase, p110*, in 3T3-L1 adipocytes. Fluorescent
detection of GLUT4 protein and actin within these cells demonstrates
that expression of p110* is sufficient to cause translocation of GLUT4
to the plasma membrane and the formation of actin membrane ruffles.
These effects are inhibited by wortmannin in the p110*-expressing
cells, indicating that the phosphatidylinositol 3-kinase activity of
the protein is required. Overexpression of an identical protein
containing a point mutation in the kinase domain, p110*
Division of Endocrinology and Metabolism,
Department of Medicine, Veterans Administration Medical Center,
University of California, San Diego, La Jolla, California 92093 and
'' Chiron Corporation, Emeryville, California 94608
kin, was
incapable of mediating either action, confirming that neither the
microinjection process nor a nonspecific effect of the protein was
responsible for the observed effects. These data suggest that although
insulin is capable of inducing numerous signaling pathways, the
isolated activation of phosphatidylinositol 3-kinase can initiate the
signaling cascade leading to both actin rearrangement and GLUT4
translocation in the absence of insulin stimulation.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Lorenzo, S. Fernandez-Veledo, R. Vila-Bedmar, L. Garcia-Guerra, C. De Alvaro, and I. Nieto-Vazquez Insulin resistance induced by tumor necrosis factor-{alpha} in myocytes and brown adipocytes J Anim Sci, April 1, 2008; 86(14_suppl): E94 - E104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Hakuno, S. Kurihara, R. T. Watson, J. E. Pessin, and S.-I. Takahashi 53BP2S, Interacting with Insulin Receptor Substrates, Modulates Insulin Signaling J. Biol. Chem., December 28, 2007; 282(52): 37747 - 37758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Liu, M. P. Jedrychowski, S. P. Gygi, and P. F. Pilch Role of Insulin-dependent Cortical Fodrin/Spectrin Remodeling in Glucose Transporter 4 Translocation in Rat Adipocytes Mol. Biol. Cell, October 1, 2006; 17(10): 4249 - 4256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Hou, S. Shigematsu, H. C. Crawford, P. Z. Anastasiadis, and J. E. Pessin Dual Regulation of Rho and Rac by p120 Catenin Controls Adipocyte Plasma Membrane Trafficking J. Biol. Chem., August 18, 2006; 281(33): 23307 - 23312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huang, T. Imamura, J. L. Babendure, J.-C. Lu, and J. M. Olefsky Disruption of Microtubules Ablates the Specificity of Insulin Signaling to GLUT4 Translocation in 3T3-L1 Adipocytes J. Biol. Chem., December 23, 2005; 280(51): 42300 - 42306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Sebastian and L. E. Nagy Decreased insulin-dependent glucose transport by chronic ethanol feeding is associated with dysregulation of the Cbl/TC10 pathway in rat adipocytes Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1077 - E1084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Y. Jiang, Q. L. Zhou, J. Holik, S. Patel, J. Leszyk, K. Coleman, M. Chouinard, and M. P. Czech Identification of WNK1 as a Substrate of Akt/Protein Kinase B and a Negative Regulator of Insulin-stimulated Mitogenesis in 3T3-L1 Cells J. Biol. Chem., June 3, 2005; 280(22): 21622 - 21628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fukuhara-Takaki, M. Sakai, Y.-i. Sakamoto, M. Takeya, and S. Horiuchi Expression of Class A Scavenger Receptor Is Enhanced by High Glucose in Vitro and under Diabetic Conditions in Vivo: ONE MECHANISM FOR AN INCREASED RATE OF ATHEROSCLEROSIS IN DIABETES J. Biol. Chem., February 4, 2005; 280(5): 3355 - 3364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sbrissa, O. C. Ikonomov, J. Strakova, and A. Shisheva Role for a Novel Signaling Intermediate, Phosphatidylinositol 5-Phosphate, in Insulin-Regulated F-Actin Stress Fiber Breakdown and GLUT4 Translocation Endocrinology, November 1, 2004; 145(11): 4853 - 4865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki, M. Furukawa, W. Raab, and J. E. Pessin Phosphatidylinositol 4,5-Bisphosphate Regulates Adipocyte Actin Dynamics and GLUT4 Vesicle Recycling J. Biol. Chem., July 16, 2004; 279(29): 30622 - 30633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bose, S. Robida, P. S. Furcinitti, A. Chawla, K. Fogarty, S. Corvera, and M. P. Czech Unconventional Myosin Myo1c Promotes Membrane Fusion in a Regulated Exocytic Pathway Mol. Cell. Biol., June 15, 2004; 24(12): 5447 - 5458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakayama, K. Mikoshiba, T. Yamamori, and K. Akagawa Activation of Syntaxin 1C, an Alternative Splice Variant of HPC-1/Syntaxin 1A, by Phorbol 12-Myristate 13-Acetate (PMA) Suppresses Glucose Transport into Astroglioma Cells via the Glucose Transporter-1 (GLUT-1) J. Biol. Chem., May 28, 2004; 279(22): 23728 - 23739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Watson, M. Kanzaki, and J. E. Pessin Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes Endocr. Rev., April 1, 2004; 25(2): 177 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. P. Thirone, J. B. C. Carvalheira, A. E. Hirata, L. A. Velloso, and M. J. A. Saad Regulation of Cbl-Associated Protein/Cbl Pathway in Muscle and Adipose Tissues of Two Animal Models of Insulin Resistance Endocrinology, January 1, 2004; 145(1): 281 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dadke and J. Chernoff Protein-tyrosine Phosphatase 1B Mediates the Effects of Insulin on the Actin Cytoskeleton in Immortalized Fibroblasts J. Biol. Chem., October 17, 2003; 278(42): 40607 - 40611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Whiteman, J. J. Chen, and M. J. Birnbaum Platelet-Derived Growth Factor (PDGF) Stimulates Glucose Transport in 3T3-L1 Adipocytes Overexpressing PDGF Receptor by a Pathway Independent of Insulin Receptor Substrates Endocrinology, September 1, 2003; 144(9): 3811 - 3820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chunqiu Hou and J. E. Pessin Lipid Raft Targeting of the TC10 Amino Terminal Domain Is Responsible for Disruption of Adipocyte Cortical Actin Mol. Biol. Cell, September 1, 2003; 14(9): 3578 - 3591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gual, T. Gonzalez, T. Gremeaux, R. Barres, Y. Le Marchand-Brustel, and J.-F. Tanti Hyperosmotic Stress Inhibits Insulin Receptor Substrate-1 Function by Distinct Mechanisms in 3T3-L1 Adipocytes J. Biol. Chem., July 11, 2003; 278(29): 26550 - 26557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tojo, I. Kaieda, H. Hattori, N. Katayama, K. Yoshimura, S. Kakimoto, Y. Fujisawa, E. Presman, C. C. Brooks, and P. F. Pilch The Formin Family Protein, Formin Homolog Overexpressed in Spleen, Interacts with the Insulin-Responsive Aminopeptidase and Profilin IIa Mol. Endocrinol., July 1, 2003; 17(7): 1216 - 1229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fujishiro, Y. Gotoh, H. Katagiri, H. Sakoda, T. Ogihara, M. Anai, Y. Onishi, H. Ono, M. Abe, N. Shojima, et al. Three Mitogen-Activated Protein Kinases Inhibit Insulin Signaling by Different Mechanisms in 3T3-L1 Adipocytes Mol. Endocrinol., March 1, 2003; 17(3): 487 - 497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ijuin and T. Takenawa SKIP Negatively Regulates Insulin-Induced GLUT4 Translocation and Membrane Ruffle Formation Mol. Cell. Biol., February 15, 2003; 23(4): 1209 - 1220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Martin, J.-P. Vincent, and J. Mazella Involvement of the Neurotensin Receptor-3 in the Neurotensin-Induced Migration of Human Microglia J. Neurosci., February 15, 2003; 23(4): 1198 - 1205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Czauderna, M. Fechtner, H. Aygun, W. Arnold, A. Klippel, K. Giese, and J. Kaufmann Functional studies of the PI(3)-kinase signalling pathway employing synthetic and expressed siRNA Nucleic Acids Res., January 15, 2003; 31(2): 670 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kossila, J. Pihlajamaki, P. Karkkainen, R. Miettinen, P. Kekalainen, I. Vauhkonen, S. Yla-Herttuala, and M. Laakso Promoter Polymorphisms -359T/C and -303A/G of the Catalytic Subunit p110{beta} Gene of Human Phosphatidylinositol 3-Kinase Are Not Associated With Insulin Secretion or Insulin Sensitivity in Finnish Subjects Diabetes Care, January 1, 2003; 26(1): 179 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tsuru, H. Katagiri, T. Asano, T. Yamada, S. Ohno, T. Ogihara, and Y. Oka Role of PKC isoforms in glucose transport in 3T3-L1 adipocytes: insignificance of atypical PKC Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E338 - E345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mora, P. L. Durham, J. R. Smith, A. F. Russo, A. Jeromin, and J. E. Pessin NCS-1 Inhibits Insulin-stimulated GLUT4 Translocation in 3T3L1 Adipocytes through a Phosphatidylinositol 4-Kinase-dependent Pathway J. Biol. Chem., July 19, 2002; 277(30): 27494 - 27500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. ZIERATH and H. WALLBERG-HENRIKSSON From Receptor to Effector: Insulin Signal Transduction in Skeletal Muscle from Type II Diabetic Patients Ann. N.Y. Acad. Sci., June 1, 2002; 967(1): 120 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Vollenweider, B. Menard, and P. Nicod Insulin Resistance, Defective Insulin Receptor Substrate 2--Associated Phosphatidylinositol-3' Kinase Activation, and Impaired Atypical Protein Kinase C ({zeta}/{lambda}) Activation in Myotubes From Obese Patients With Impaired Glucose Tolerance Diabetes, April 1, 2002; 51(4): 1052 - 1059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Y. Jiang, A. Chawla, A. Bose, M. Way, and M. P. Czech A Phosphatidylinositol 3-Kinase-independent Insulin Signaling Pathway to N-WASP/Arp2/3/F-actin Required for GLUT4 Glucose Transporter Recycling J. Biol. Chem., January 4, 2002; 277(1): 509 - 515. [Abstract] [Full Text]
|
|
|
|
|
|
S. F. Kralik, P. Liu, B. J. Leffler, and J. S. Elmendorf Ceramide and Glucosamine Antagonism of Alternate Signaling Pathways Regulating Insulin- and Osmotic Shock-Induced Glucose Transporter 4 Translocation Endocrinology, January 1, 2002; 143(1): 37 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Pagliassotti, J. Kang, J. S. Thresher, C. K. Sung, and M. E. Bizeau Elevated basal PI 3-kinase activity and reduced insulin signaling in sucrose-induced hepatic insulin resistance Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E170 - E176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Somwar, W. Niu, D. Y. Kim, G. Sweeney, V. K. Randhawa, C. Huang, T. Ramlal, and A. Klip Differential Effects of Phosphatidylinositol 3-Kinase Inhibition on Intracellular Signals Regulating GLUT4 Translocation and Glucose Transport J. Biol. Chem., November 30, 2001; 276(49): 46079 - 46087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki and J. E. Pessin Insulin-stimulated GLUT4 Translocation in Adipocytes Is Dependent upon Cortical Actin Remodeling J. Biol. Chem., November 2, 2001; 276(45): 42436 - 42444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Teruel, R. Hernandez, and M. Lorenzo Ceramide Mediates Insulin Resistance by Tumor Necrosis Factor-{alpha} in Brown Adipocytes by Maintaining Akt in an Inactive Dephosphorylated State Diabetes, November 1, 2001; 50(11): 2563 - 2571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Boileau, M. Cauzac, M. A. Pereira, J. Girard, and S. Hauguel-de Mouzon Dissociation between Insulin-Mediated Signaling Pathways and Biological Effects in Placental Cells: Role of Protein Kinase B and MAPK Phosphorylation Endocrinology, September 1, 2001; 142(9): 3974 - 3979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bose, A. D. Cherniack, S. E. Langille, S. M. C. Nicoloro, J. M. Buxton, J. G. Park, A. Chawla, and M. P. Czech G{alpha}11 Signaling through ARF6 Regulates F-Actin Mobilization and GLUT4 Glucose Transporter Translocation to the Plasma Membrane Mol. Cell. Biol., August 1, 2001; 21(15): 5262 - 5275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Takano, T. Haruta, M. Iwata, I. Usui, T. Uno, J. Kawahara, E. Ueno, T. Sasaoka, and M. Kobayashi Growth Hormone Induces Cellular Insulin Resistance by Uncoupling Phosphatidylinositol 3-Kinase and Its Downstream Signals in 3T3-L1 Adipocytes Diabetes, August 1, 2001; 50(8): 1891 - 1900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kimura, C. A. Baumann, S.-H. Chiang, and A. R. Saltiel The sorbin homology domain: A motif for the targeting of proteins to lipid rafts PNAS, July 31, 2001; 98(16): 9098 - 9103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Iwata, T. Haruta, I. Usui, Y. Takata, A. Takano, T. Uno, J. Kawahara, E. Ueno, T. Sasaoka, O. Ishibashi, et al. Pioglitazone Ameliorates Tumor Necrosis Factor-{alpha}-Induced Insulin Resistance by a Mechanism Independent of Adipogenic Activity of Peroxisome Proliferator-Activated Receptor-{gamma} Diabetes, May 1, 2001; 50(5): 1083 - 1092. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Cox, B. M. Dale, M. Kashiwada, C. D. Helgason, and S. Greenberg A Regulatory Role for Src Homology 2 Domain-containing Inositol 5'-Phosphatase (SHIP) in Phagocytosis Mediated by Fc{{gamma}} Receptors and Complement Receptor 3 ({{alpha}}M{beta}2; CD11b/CD18) J. Exp. Med., December 27, 2000; 193(1): 61 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Kriauciunas, M. G. Myers Jr., and C. R. Kahn Cellular Compartmentalization in Insulin Action: Altered Signaling by a Lipid-Modified IRS-1 Mol. Cell. Biol., September 15, 2000; 20(18): 6849 - 6859. [Abstract] [Full Text]
|
|
|
|
|
|
K. Egawa, N. Nakashima, P. M. Sharma, H. Maegawa, Y. Nagai, A. Kashiwagi, R. Kikkawa, and J. M. Olefsky Persistent Activation of Phosphatidylinositol 3-Kinase Causes Insulin Resistance Due to Accelerated Insulin-Induced Insulin Receptor Substrate-1 Degradation in 3T3-L1 Adipocytes Endocrinology, June 1, 2000; 141(6): 1930 - 1935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nakashima, P. M. Sharma, T. Imamura, R. Bookstein, and J. M. Olefsky The Tumor Suppressor PTEN Negatively Regulates Insulin Signaling in 3T3-L1 Adipocytes J. Biol. Chem., April 21, 2000; 275(17): 12889 - 12895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tong, W. Chen, R. E. London, E. Murphy, and C. Steenbergen Preconditioning Enhanced Glucose Uptake Is Mediated by p38 MAP Kinase Not by Phosphatidylinositol 3-Kinase J. Biol. Chem., April 14, 2000; 275(16): 11981 - 11986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki, R. T. Watson, N. O. Artemyev, and J. E. Pessin The Trimeric GTP-binding Protein (Gq/G11) alpha Subunit Is Required for Insulin-stimulated GLUT4 Translocation in 3T3L1 Adipocytes J. Biol. Chem., March 15, 2000; 275(10): 7167 - 7175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. SUMMERS, V. P. YIN, E. L. WHITEMAN, L. A. GARZA, H. CHO, R. L. TUTTLE, and M. J. BIRNBAUM Signaling Pathways Mediating Insulin-Stimulated Glucose Transport Ann. N.Y. Acad. Sci., November 18, 1999; 892(1): 169 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Hill, S. F. Clark, D. F. Tucker, M. J. Birnbaum, D. E. James, and S. L. Macaulay A Role for Protein Kinase Bbeta /Akt2 in Insulin-Stimulated GLUT4 Translocation in Adipocytes Mol. Cell. Biol., November 1, 1999; 19(11): 7771 - 7781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. P. Foran, L. M. Fletcher, P. B. Oatey, N. Mohammed, J. O. Dolly, and J. M. Tavare Protein Kinase B Stimulates the Translocation of GLUT4 but Not GLUT1 or Transferrin Receptors in 3T3-L1 Adipocytes by a Pathway Involving SNAP-23, Synaptobrevin-2, and/or Cellubrevin J. Biol. Chem., October 1, 1999; 274(40): 28087 - 28095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Funaki, H. Katagiri, A. Kanda, M. Anai, M. Nawano, T. Ogihara, K. Inukai, Y. Fukushima, H. Ono, Y. Yazaki, et al. p85/p110-type Phosphatidylinositol Kinase Phosphorylates Not Only the D-3, but Also the D-4 Position of the Inositol Ring J. Biol. Chem., July 30, 1999; 274(31): 22019 - 22024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takasuga, T. Katada, M. Ui, and O. Hazeki Enhancement by Adenosine of Insulin-induced Activation of Phosphoinositide 3-Kinase and Protein Kinase B in Rat Adipocytes J. Biol. Chem., July 9, 1999; 274(28): 19545 - 19550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang, R. Somwar, P. J. Bilan, Z. Liu, J. Jin, J. R. Woodgett, and A. Klip Protein Kinase B/Akt Participates in GLUT4 Translocation by Insulin in L6 Myoblasts Mol. Cell. Biol., June 1, 1999; 19(6): 4008 - 4018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Egawa, P. M. Sharma, N. Nakashima, Y. Huang, E. Huver, G. R. Boss, and J. M. Olefsky Membrane-targeted Phosphatidylinositol 3-Kinase Mimics Insulin Actions and Induces a State of Cellular Insulin Resistance J. Biol. Chem., May 14, 1999; 274(20): 14306 - 14314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Foster, K. Yaworsky, W. S. Trimble, and A. Klip SNAP23 promotes insulin-dependent glucose uptake in 3T3-L1 adipocytes: possible interaction with cytoskeleton Am J Physiol Cell Physiol, May 1, 1999; 276(5): C1108 - C1114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tirosh, R. Potashnik, N. Bashan, and A. Rudich Oxidative Stress Disrupts Insulin-induced Cellular Redistribution of Insulin Receptor Substrate-1 and Phosphatidylinositol 3-Kinase in 3T3-L1 Adipocytes. A PUTATIVE CELLULAR MECHANISM FOR IMPAIRED PROTEIN KINASE B ACTIVATION AND GLUT4 TRANSLOCATION J. Biol. Chem., April 9, 1999; 274(15): 10595 - 10602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Craddock, E. A. Orchiston, H. J. Hinton, and M. J. Welham Dissociation of Apoptosis from Proliferation, Protein Kinase B Activation, and BAD Phosphorylation in Interleukin-3-mediated Phosphoinositide 3-Kinase Signaling J. Biol. Chem., April 9, 1999; 274(15): 10633 - 10640. [Abstract] [Full Text] [PDF] |
