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Volume 271, Number 30, Issue of July 26, 1996 pp. 18054-18060
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Plasma Lipopolysaccharide-binding Protein Is Found Associated with a Particle Containing Apolipoprotein A-I, Phospholipid, and Factor H-related Proteins

(Received for publication, November 27, 1995, and in revised form, March 11, 1996)

From the Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, New York 10021

Neutrophils exhibit a dramatic enhancement of integrin-mediated cell adhesion in response to lipopolysaccharide (LPS). This response requires CD14 on the neutrophil and plasma proteins in solution. We have purified the factor from plasma that facilitates the adhesive response of neutrophil to LPS by using a combination of affinity and ion-exchange chromatography. Previous work has shown that the activity is associated with apolipoprotein A-I (apoA-I), and here we show that this activity is associated with an apoA-I-bearing complex of protein and phospholipid. Native polyacrylamide gel electrophoresis (PAGE) analysis showed a ladder of bands in the M_r 200,000 region, and electron microscopy revealed round, indented particles of 11.4 ± 0.12 nm in diameter. Characterization of these particles revealed a density of 1.219-1.264 g/ml and ~10 molecules of lipid phosphate per M_r 200,000 complex. SDS-PAGE showed that each of the bands seen in native PAGE was composed of several polypeptides. These were identified as apoA-I, LPS binding protein (LBP), and factor H-related proteins (FHRPs). Physical association of apoA-I, LBP, and FHRP in these particles was further confirmed using double immunodiffusion, and association of LBP and FHRP in plasma was confirmed by coimmunoprecipitation. FHRPs are the numerically dominant protein components in these particles, and all plasma FHRP-1 appears to be associated with these particles. We suggest that FHRPs may be the defining constituent of this novel ``lipoprotein'' particle.

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