HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zelicof, A. Articles by Gerst, J. E. Search for Related Content PubMed PubMed Citation Articles by Zelicof, A. Articles by Gerst, J. E. Social Bookmarking                      What's this?

Volume 271, Number 30, Issue of July 26, 1996 pp. 18243-18252
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Two Separate Functions Are Encoded by the Carboxyl-terminal Domains of the Yeast Cyclase-associated Protein and Its Mammalian Homologs
DIMERIZATION AND ACTIN BINDING

(Received for publication, March 12, 1996, and in revised form, April 15, 1996)

Audrey Zelicof , Vladimir Protopopov , Doris David ^§ , Xue-Ying Lin , Vardit Lustgarten ^§ and Jeffrey E. Gerst ^§

From the  Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, New York 10029 and the ^§ Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel

The yeast adenylyl cyclase-associated protein, CAP, was identified as a component of the RAS-activated cyclase complex. CAP consists of two functional domains separated by a proline-rich region. One domain, which localizes to the amino terminus, mediates RAS signaling through adenylyl cyclase, while a domain at the carboxyl terminus is involved in the regulation of cell growth and morphogenesis. Recently, the carboxyl terminus of yeast CAP was shown to sequester actin, but whether this function has been conserved, and is the sole function of this domain, is unclear. Here, we demonstrate that the carboxyl-terminal domains of CAP and CAP homologs have two separate functions. We show that carboxyl-terminals of both yeast CAP and a mammalian CAP homolog, MCH1, bind to actin. We also show that this domain contains a signal for dimerization, allowing both CAP and MCH1 to form homodimers and heterodimers. The properties of actin binding and dimerization are mediated by separate regions on the carboxyl terminus; the last 27 amino acids of CAP being critical for actin binding. Finally, we present evidence that links a segment of the proline-rich region of CAP to its localization in yeast. Together, these results suggest that all three domains of CAP proteins are functional.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				O. Quintero-Monzon, E. M. Jonasson, E. Bertling, L. Talarico, F. Chaudhry, M. Sihvo, P. Lappalainen, and B. L. Goode Reconstitution and Dissection of the 600-kDa Srv2/CAP Complex: ROLES FOR OLIGOMERIZATION AND COFILIN-ACTIN BINDING IN DRIVING ACTIN TURNOVER J. Biol. Chem., April 17, 2009; 284(16): 10923 - 10934. [Abstract] [Full Text] [PDF]

				F. Chaudhry, C. Guerin, M. von Witsch, L. Blanchoin, and C. J. Staiger Identification of Arabidopsis Cyclase-associated Protein 1 as the First Nucleotide Exchange Factor for Plant Actin Mol. Biol. Cell, August 1, 2007; 18(8): 3002 - 3014. [Abstract] [Full Text] [PDF]

				Y.-S. Bahn, J. K. Hicks, S. S. Giles, G. M. Cox, and J. Heitman Adenylyl Cyclase-Associated Protein Aca1 Regulates Virulence and Differentiation of Cryptococcus neoformans via the Cyclic AMP-Protein Kinase A Cascade Eukaryot. Cell, December 1, 2004; 3(6): 1476 - 1491. [Abstract] [Full Text] [PDF]

				P. K. Mattila, O. Quintero-Monzon, J. Kugler, J. B. Moseley, S. C. Almo, P. Lappalainen, and B. L. Goode A High-affinity Interaction with ADP-Actin Monomers Underlies the Mechanism and In Vivo Function of Srv2/cyclase-associated Protein Mol. Biol. Cell, November 1, 2004; 15(11): 5158 - 5171. [Abstract] [Full Text] [PDF]

				R. A. BARRERO, M. UMEDA, S. YAMAMURA, and H. UCHIMIYA Over-expression of Arabidopsis CAP Causes Decreased Cell Expansion Leading to Organ Size Reduction in Transgenic Tobacco Plants Ann. Bot., April 1, 2003; 91(5): 599 - 603. [Abstract] [Full Text] [PDF]

				S. Gurunathan, M. Marash, A. Weinberger, and J. E. Gerst t-SNARE Phosphorylation Regulates Endocytosis in Yeast Mol. Biol. Cell, May 1, 2002; 13(5): 1594 - 1607. [Abstract] [Full Text] [PDF]

				K. Moriyama and I. Yahara Human CAP1 is a key factor in the recycling of cofilin and actin for rapid actin turnover J. Cell Sci., April 15, 2002; 115(8): 1591 - 1601. [Abstract] [Full Text] [PDF]

				A. V. HUBBERSTEY and E. P. MOTTILLO Cyclase-associated proteins: CAPacity for linking signal transduction and actin polymerization FASEB J, April 1, 2002; 16(6): 487 - 499. [Abstract] [Full Text] [PDF]

				R. A. Barrero, M. Umeda, S. Yamamura, and H. Uchimiya Arabidopsis CAP Regulates the Actin Cytoskeleton Necessary for Plant Cell Elongation and Division PLANT CELL, January 1, 2002; 14(1): 149 - 163. [Abstract] [Full Text] [PDF]

				Y.-S. Bahn and P. Sundstrom CAP1, an Adenylate Cyclase-Associated Protein Gene, Regulates Bud-Hypha Transitions, Filamentous Growth, and Cyclic AMP Levels and Is Required for Virulence of Candida albicans J. Bacteriol., May 15, 2001; 183(10): 3211 - 3223. [Abstract] [Full Text]

				S. Gurunathan, D. Chapman-Shimshoni, S. Trajkovic, and J. E. Gerst Yeast Exocytic v-SNAREs Confer Endocytosis Mol. Biol. Cell, October 1, 2000; 11(10): 3629 - 3643. [Abstract] [Full Text]

				J. A. Surtees and B. E. Funnell P1 ParB Domain Structure Includes Two Independent Multimerization Domains J. Bacteriol., October 1, 1999; 181(19): 5898 - 5908. [Abstract] [Full Text]

				J. Yu, C. Wang, S. J. Palmieri, B. K. Haarer, and J. Field A Cytoskeletal Localizing Domain in the Cyclase-associated Protein, CAP/Srv2p, Regulates Access to a Distant SH3-binding Site J. Biol. Chem., July 9, 1999; 274(28): 19985 - 19991. [Abstract] [Full Text] [PDF]

				V. Lustgarten and J. E. Gerst Yeast VSM1 Encodes a v-SNARE Binding Protein That May Act as a Negative Regulator of Constitutive Exocytosis Mol. Cell. Biol., June 1, 1999; 19(6): 4480 - 4494. [Abstract] [Full Text] [PDF]

				D. David, S. Sundarababu, and J. E. Gerst Involvement of Long Chain Fatty Acid Elongation in the Trafficking of Secretory Vesicles in Yeast J. Cell Biol., November 30, 1998; 143(5): 1167 - 1182. [Abstract] [Full Text] [PDF]

				Y. Nishida, F. Shima, H. Sen, Y. Tanaka, C. Yanagihara, Y. Yamawaki-Kataoka, K.-i. Kariya, and T. Kataoka Coiled-coil Interaction of N-terminal 36 Residues of Cyclase-associated Protein with Adenylyl Cyclase Is Sufficient for Its Function in Saccharomyces cerevisiae Ras Pathway J. Biol. Chem., October 23, 1998; 273(43): 28019 - 28024. [Abstract] [Full Text] [PDF]

				J. E. Gerst Conserved alpha -Helical Segments on Yeast Homologs of the Synaptobrevin/VAMP Family of v-SNAREs Mediate Exocytic Function J. Biol. Chem., June 27, 1997; 272(26): 16591 - 16598. [Abstract] [Full Text] [PDF]

				C. P. Ponting, R. Mott, P. Bork, and R. R. Copley Novel Protein Domains and Repeats in Drosophila melanogaster: Insights into Structure, Function, and Evolution Genome Res., December 1, 2001; 11(12): 1996 - 2008. [Abstract] [Full Text] [PDF]