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Volume 271, Number 31, Issue of August 2, 1996 pp. 18485-18493
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Condensation of Rat Telomere-specific Nucleosomal Arrays Containing Unusually Short DNA Repeats and Histone H1

(Received for publication, March 20, 1996, and in revised form, May 16, 1996)

Jirair K. Bedoyan ^§ , Serguei Lejnine ^§ , Vladimir L. Makarov ^§ and John P. Langmore ^§

From the  Graduate Program in Cellular and Molecular Biology, ^§ Biophysics Research Division, and  Department of Biological Sciences, The University of Michigan, Ann Arbor, Michigan 48109-1055

Vertebrate telomeres contain arrays of nucleosomes with unusually short and regular repeat lengths (Makarov, V. L., Lejnine, S., Bedoyan, J., and Langmore, J. P. (1993) Cell 73, 775-787; Lejnine, S., Makarov, V., and Langmore, J. P. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 2393-2397). In order to better define the specific structural features of telomere chromatin, we examined the condensation and H1 content of telomere nucleoproteins from rat liver. Velocity sedimentation analysis shows that telomeric nucleosome arrays condense with increasing ionic strength and molecular weight in a manner comparable with that of bulk chromatin despite the very short repeat length. However, these condensed structures do not exhibit the ~100-base pair deoxyribonuclease II repeat characteristic of condensed bulk chromatin. Frictional coefficient calculations suggest that telomere-specific higher order structure is more compact than bulk chromatin. Nucleoprotein gel electrophoresis shows that telomeric dinucleosomes from soluble chromatin contain H1. Finally, direct isolation and analysis of telomere nucleoproteins from formaldehyde-cross-linked nuclei indicate the presence of core histone proteins and H1. These results are consistent with the view that a major fraction of the long telomeres of rat are organized as specialized nucleosome arrays with features similar but not identical to those of bulk chromatin.

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