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Volume 271, Number 31, Issue of August 2, 1996 pp. 18885-18891
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Antisense RNA, Fur, Iron, and the Regulation of Iron Transport Genes in Vibrio anguillarum

(Received for publication, February 13, 1996, and in revised form, May 7, 1996)

From the Department of Molecular Microbiology and Immunology L220, School of Medicine, Oregon Health Sciences University, Portland, Oregon 97201-3098

The negative regulation of the expression of iron transport genes fatA and fatB in Vibrio anguillarum is mediated by a chromosome-encoded Fur protein and a plasmid pJM1-derived antisense RNA (RNA), which is preferentially expressed under iron-rich conditions. In this work, we characterized the RNA promoter region, and by using promoter fusion and rifampicin experiments we were able to demonstrate that iron regulates RNA synthesis posttranscriptionally by stabilizing RNA half-life rather than enhancing transcription initiation. The Fur protein is also essential for RNA synthesis at the transcription initiation level, independently of the iron status of the cell. From experiments assessing the relative contribution of Fur and RNA, we were able to show that RNA may indeed play an important role on the negative regulation of the expression of the iron transport genes under physiological conditions.

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