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Volume 271, Number 32, Issue of August 9, 1996 pp. 19093-19098
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Expression of Kv1.1 Delayed Rectifier Potassium Channels in Lec Mutant Chinese Hamster Ovary Cell Lines Reveals a Role for Sialidation in Channel Function

(Received for publication, March 19, 1996, and in revised form, May 16, 1996)

From the Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029

Kv1.1 potassium (K⁺) channels contain significant amounts of negatively charged sialic acids. To examine the role of sialidation in K⁺ channel function, Chinese hamster ovary cell lines deficient in glycosylation (Lec mutants) were transfected with rat brain Kv1.1 cDNA. The K⁺ channel was functionally expressed in all cell lines, but the voltage dependence of activation (V1/2) was shifted to more positive voltages and the activation kinetics were slower in the mutant cell lines compared with control. A similar positive shift in V1/2 was recorded in control cells expressing Kv1.1 following treatment with sialidase or by raising extracellular Ca²⁺. In contrast, these treatments had little or no effect on the Lec mutants, which indicates that channel sialic acids appear to be the negative surface charges sensitive to Ca²⁺. The data suggest that sialic acid addition modifies Kv1.1 channel function, possibly by influencing the local electric field detected by its voltage sensor, but that these carbohydrates are not required for cell surface expression.

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