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Volume 271, Number 32, Issue of August 9, 1996 pp. 19174-19179
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Structural Organization of the Reduced Folate Carrier Gene in Chinese Hamster Ovary Cells

(Received for publication, March 6, 1996, and in revised form, May 24, 1996)

From the Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada

The reduced folate carrier gene (rfc) encodes a putative protein that is involved in the intracellular accumulation of folates. In this report, we describe the organization of the rfc gene from Chinese hamster ovary cells. The hamster rfc gene contains 7 exons and 6 introns, which span 15.3 kilobases. It codes for two alternatively spliced messenger RNAs, one that contains all 7 exons and one that lacks exon 2 but contains the remaining 6 exons. The transcriptional start of the gene has been mapped to six sites approximately 200 base pairs upstream of the putative ATG initiation codon. The promoter region has no TATA box-like sequence but contains a consensus Sp1 binding site. This is the first report of the genomic structure of the reduced folate carrier gene from any species.

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