| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, April 26, 1996, and in revised form, May 17, 1996)
From the Department of Molecular Glycobiology, Frontier Research
Program, The Institute of Physical and Chemical Research (RIKEN), Wako,
Saitama 351-01, Japan
We previously showed that mouse ST8Sia II (STX)
exhibits polysialic acid (PSA) synthase activity in vivo as
well as in vitro (Kojima, N., Yoshida, Y., and Tsuji, S. (1995) FEBS Lett. 373, 119-122, 1995). In this paper, we
reported that the neural cell adhesion molecule (NCAM) was specifically
polysialylated by a single enzyme, ST8Sia II. PSA-expressing Neuro2a
cells (N2a-STX) were established by stable transfection of the mouse
ST8Sia II gene. Only the 140- and 180-kDa isoforms of NCAM in N2a-STX
cells were specifically polysialylated in vivo, although
other membrane proteins of N2a-STX were polysialylated in
vitro. A recombinant soluble mouse ST8Sia II synthesized PSA on a
recombinant soluble NCAM fused with the Fc region of human IgG1
(NCAM-Fc) as well as fetuin. However, NCAM-Fc served as a 1500-fold
better acceptor for ST8Sia II than fetuin. Treatment of NCAM-Fc with
Charonia lampas
Volume 271, Number 32,
Issue of August 9, 1996
pp. 19457-19463
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
REQUIREMENT OF CORE 
1,6-LINKED FUCOSE AND A POLYPEPTIDE CHAIN
FOR POLYSIALYLATION
-fucosidase, which is able to cleave
1,6-linked fucose, clearly reduced the polysialylation of NCAM-Fc by
ST8Sia II. PSA was not synthesized on the
N-glycanase-treated NCAM-Fc polypeptide or the free
N-glycans of NCAM-Fc. When fetuin and its glycopeptide and
N-glycans of fetuin were used as substrates for ST8Sia II,
PSA was found to be synthesized on native fetuin and its glycopeptide
but not on free N-glycans. These results strongly suggested
that core 1,6-fucose on N-glycans as well as the
antennary structures of N-glycans and the polypeptide
regions are required for the polysialylation by ST8Sia II. Furthermore,
oligo and single 2,8-sialylated glycoproteins were no longer
polysialylated by mouse ST8Sia II. Therefore, the single enzyme, ST8Sia
II, directly transferred all 2,8-sialic acid residues on the
2,3-linked sialic acids of N-glycans of specific NCAM
isoforms to yield PSA-NCAM. Polysialylation did not require any
initiator 2,8-sialyltransferase but did depend on the carbohydrate
and protein structures of NCAM.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Oltmann-Norden, S. P. Galuska, H. Hildebrandt, R. Geyer, R. Gerardy-Schahn, H. Geyer, and M. Muhlenhoff Impact of the Polysialyltransferases ST8SiaII and ST8SiaIV on Polysialic Acid Synthesis during Postnatal Mouse Brain Development J. Biol. Chem., January 18, 2008; 283(3): 1463 - 1471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Galuska, R. Geyer, R. Gerardy-Schahn, M. Muhlenhoff, and H. Geyer Enzyme-dependent Variations in the Polysialylation of the Neural Cell Adhesion Molecule (NCAM) in Vivo J. Biol. Chem., January 4, 2008; 283(1): 17 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Wierinckx, C. Auger, P. Devauchelle, A. Reynaud, P. Chevallier, M. Jan, G. Perrin, M. Fevre-Montange, C. Rey, D. Figarella-Branger, et al. A diagnostic marker set for invasion, proliferation, and aggressiveness of prolactin pituitary tumors Endocr. Relat. Cancer, September 1, 2007; 14(3): 887 - 900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Galuska, I. Oltmann-Norden, H. Geyer, B. Weinhold, K. Kuchelmeister, H. Hildebrandt, R. Gerardy-Schahn, R. Geyer, and M. Muhlenhoff Polysialic Acid Profiles of Mice Expressing Variant Allelic Combinations of the Polysialyltransferases ST8SiaII and ST8SiaIV J. Biol. Chem., October 20, 2006; 281(42): 31605 - 31615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Close, S. S. Mendiratta, K. M. Geiger, L. J. Broom, L.-L. Ho, and K. J. Colley The Minimal Structural Domains Required for Neural Cell Adhesion Molecule Polysialylation by PST/ST8Sia IV and STX/ST8Sia II J. Biol. Chem., August 15, 2003; 278(33): 30796 - 30805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Angata, M. Suzuki, and M. Fukuda ST8Sia II and ST8Sia IV Polysialyltransferases Exhibit Marked Differences in Utilizing Various Acceptors Containing Oligosialic Acid and Short Polysialic Acid. THE BASIS FOR COOPERATIVE POLYSIALYLATION BY TWO ENZYMES J. Biol. Chem., September 20, 2002; 277(39): 36808 - 36817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. von der Ohe, S. F. Wheeler, M. Wuhrer, D. J. Harvey, S. Liedtke, M. Muhlenhoff, R. Gerardy-Schahn, H. Geyer, R. A. Dwek, R. Geyer, et al. Localization and characterization of polysialic acid-containing N-linked glycans from bovine NCAM Glycobiology, January 1, 2002; 12(1): 47 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Sterling, H. F. Quigley, A. Orellana, and D. Mohnen The Catalytic Site of the Pectin Biosynthetic Enzyme {alpha}-1,4-Galacturonosyltransferase Is Located in the Lumen of the Golgi Plant Physiology, September 1, 2001; 127(1): 360 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Franceschini, K. Angata, E. Ong, A. Hong, P. Doherty, and M. Fukuda Polysialyltransferase ST8Sia II (STX) polysialylates all of the major isoforms of NCAM and facilitates neurite outgrowth Glycobiology, March 1, 2001; 11(3): 231 - 239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Javaud, F. Dupuy, A. Maftah, J.-C. Michalski, R. Oriol, J.-M. Petit, and R. Julien Ancestral Exonic Organization of FUT8, the Gene Encoding the {alpha}6-Fucosyltransferase, Reveals Successive Peptide Domains Which Suggest a Particular Three-Dimensional Core Structure for the {alpha}6-Fucosyltransferase Family Mol. Biol. Evol., November 1, 2000; 17(11): 1661 - 1672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Charter, L. K. Mahal, D. E. Koshland Jr., and C. R. Bertozzi Biosynthetic incorporation of unnatural sialic acids into polysialic acid on neural cells Glycobiology, October 1, 2000; 10(10): 1049 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Tanaka, Y. Otake, T. Nakagawa, Y. Kawano, R. Miyahara, M. Li, K. Yanagihara, J. Nakayama, I. Fujimoto, K. Ikenaka, et al. Expression of Polysialic Acid and STX, a Human Polysialyltransferase, Is Correlated with Tumor Progression in Non-Small Cell Lung Cancer Cancer Res., June 1, 2000; 60(11): 3072 - 3080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Soares, Y. von Boxberg, M. Ravaille-Veron, J.-D. Vincent, and F. Nothias Morphofunctional Plasticity in the Adult Hypothalamus Induces Regulation of Polysialic Acid-Neural Cell Adhesion Molecule through Changing Activity and Expression Levels of Polysialyltransferases J. Neurosci., April 1, 2000; 20(7): 2551 - 2557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. F. Rafuse and L. T. Landmesser The Pattern of Avian Intramuscular Nerve Branching Is Determined by the Innervating Motoneuron and Its Level of Polysialic Acid J. Neurosci., February 1, 2000; 20(3): 1056 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Miyoshi, K. Noda, J. H. Ko, A. Ekuni, T. Kitada, N. Uozumi, Y. Ikeda, N. Matsuura, Y. Sasaki, N. Hayashi, et al. Overexpression of {{alpha}}1-6 Fucosyltransferase in Hepatoma Cells Suppresses Intrahepatic Metastasis after Splenic Injection in Athymic Mice Cancer Res., May 1, 1999; 59(9): 2237 - 2243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Close and K. J. Colley In Vivo Autopolysialylation and Localization of the Polysialyltransferases PST and STX J. Biol. Chem., December 18, 1998; 273(51): 34586 - 34593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Angata, M. Suzuki, and M. Fukuda Differential and Cooperative Polysialylation of the Neural Cell Adhesion Molecule by Two Polysialyltransferases, PST and STX J. Biol. Chem., October 23, 1998; 273(43): 28524 - 28532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takashima, Y. Yoshida, T. Kanematsu, N. Kojima, and S. Tsuji Genomic Structure and Promoter Activity of the Mouse Polysialic Acid Synthase (mST8Sia IV/PST) Gene J. Biol. Chem., March 27, 1998; 273(13): 7675 - 7683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Bruses and U. Rutishauser Regulation of Neural Cell Adhesion Molecule Polysialylation: Evidence for Nontranscriptional Control and Sensitivity to an Intracellular Pool of Calcium J. Cell Biol., March 9, 1998; 140(5): 1177 - 1186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Srikrishna, N. M. Varki, P. C. Newell, A. Varki, and H. H. Freeze An IgG Monoclonal Antibody against Dictyostelium discoideum Glycoproteins Specifically Recognizes Fucalpha 1,6GlcNAcbeta in the Core of N-Linked Glycans. LOCALIZED EXPRESSION OF CORE-FUCOSYLATED GLYCOCONJUGATES IN HUMAN TISSUES J. Biol. Chem., October 10, 1997; 272(41): 25743 - 25752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Angata, J. Nakayama, B. Fredette, K. Chong, B. Ranscht, and M. Fukuda Human STX Polysialyltransferase Forms the Embryonic Form of the Neural Cell Adhesion Molecule. TISSUE-SPECIFIC EXPRESSION, NEURITE OUTGROWTH, AND CHROMOSOMAL LOCALIZATION IN COMPARISON WITH ANOTHER POLYSIALYLTRANSFERASE, PST J. Biol. Chem., March 14, 1997; 272(11): 7182 - 7190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yoshida, N. Kurosawa, T. Kanematsu, N. Kojima, and S. Tsuji Genomic Structure and Promoter Activity of the Mouse Polysialic Acid Synthase Gene (mST8SiaII). BRAIN-SPECIFIC EXPRESSION FROM A TATA-LESS GC-RICH SEQUENCE J. Biol. Chem., November 22, 1996; 271(47): 30167 - 30173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kono, Y. Yoshida, N. Kojima, and S. Tsuji Molecular Cloning and Expression of a Fifth Type of alpha 2,8-Sialyltransferase (ST8Sia V). ITS SUBSTRATE SPECIFICITY IS SIMILAR TO THAT OF SAT-V/III, WHICH SYNTHESIZE GD1c, GT1a, GQ1b AND GT3 J. Biol. Chem., November 15, 1996; 271(46): 29366 - 29371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kojima, M. Kono, Y. Yoshida, Y. Tachida, M. Nakafuku, and S. Tsuji Biosynthesis and Expression of Polysialic Acid on the Neural Cell Adhesion Molecule Is Predominantly Directed by ST8Sia II/STX during in Vitro Neuronal Differentiation J. Biol. Chem., September 6, 1996; 271(36): 22058 - 22062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kitazume-Kawaguchi, S. Kabata, and M. Arita Differential Biosynthesis of Polysialic or Disialic Acid Structure by ST8Sia II and ST8Sia IV J. Biol. Chem., May 4, 2001; 276(19): 15696 - 15703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Legaigneur, C. Breton, A. El Battari, J.-C. Guillemot, C. Auge, M. Malissard, E. G. Berger, and C. Ronin Exploring the Acceptor Substrate Recognition of the Human beta -Galactoside alpha 2,6-Sialyltransferase J. Biol. Chem., June 8, 2001; 276(24): 21608 - 21617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Inoue and Y. Inoue Developmental Profile of Neural Cell Adhesion Molecule Glycoforms with a Varying Degree of Polymerization of Polysialic Acid Chains J. Biol. Chem., August 17, 2001; 276(34): 31863 - 31870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Angata, M. Suzuki, J. McAuliffe, Y. Ding, O. Hindsgaul, and M. Fukuda Differential Biosynthesis of Polysialic Acid on Neural Cell Adhesion Molecule (NCAM) and Oligosaccharide Acceptors by Three Distinct alpha 2,8-Sialyltransferases, ST8Sia IV (PST), ST8Sia II (STX), and ST8Sia III J. Biol. Chem., June 9, 2000; 275(24): 18594 - 18601. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
