JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Si, J.
Right arrow Articles by Mei, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Si, J.
Right arrow Articles by Mei, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 271, Number 33, Issue of August 16, 1996 pp. 19752-19759
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

Induction of Acetylcholine Receptor Gene Expression by ARIA Requires Activation of Mitogen-activated Protein Kinase

(Received for publication, March 28, 1996, and in revised form, May 31, 1996)

Jutong Si Dagger , Zhijun Luo § and Lin Mei Dagger

From the Dagger  Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia 22908 and the § Diabetes Unit, Massachusetts General Hospital, Charlestown, Massachusetts 02129

Transcription of genes encoding nicotinic acetylcholine receptor (AChR) subunits (alpha , beta , gamma  or epsilon , and delta ) is highest in nuclei localized to the synaptic region of the muscle, which contributes to maintain a high density of AChRs at the postjunctional membrane. ARIA (AChR inducing activity) is believed to be the trophic factor utilized by motor neurons to stimulate AChR synthesis in the subsynaptic area. To elucidate the signaling mechanism initiated by ARIA, we established stable C2C12 cell lines carrying the nuclear lacZ gene under the control of the mouse epsilon  subunit promoter or chicken alpha  subunit promoter. ARIA stimulated tyrosine phosphorylation of erbB proteins in these C2C12 cells within 15 s with a peak at 5 min. Immediately following tyrosine phosphorylation of erbB proteins, mitogen-activated protein (MAP) kinase was activated which occurred within 30 s and peaked at 8 min after ARIA stimulation. Concomitantly, expression of AChR genes was induced by ARIA. ARIA-induced AChR subunit transgene expression was observed only in differentiated myotubes and not in myoblasts, suggesting that downstream signaling component(s) are regulated in a manner dependent on the myogenic program. Inhibition of the MAP kinase activity by using a specific MAP kinase kinase inhibitor or by overexpressing dominant negative mutants of Raf or MAP kinase kinase attenuated or abolished the ARIA-induced activation of AChR alpha  and epsilon  subunit gene expression. These results indicate that regulation of AChR gene expression by ARIA in C2C12 cells requires activation of the MAP kinase signaling pathway.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
D. Zhu, Z. Yang, Z. Luo, S. Luo, W. C. Xiong, and L. Mei
Muscle-Specific Receptor Tyrosine Kinase Endocytosis in Acetylcholine Receptor Clustering in Response to Agrin
J. Neurosci., February 13, 2008; 28(7): 1688 - 1696.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Dai, W. C. Xiong, and L. Mei
Erbin Inhibits RAF Activation by Disrupting the Sur-8-Ras-Raf Complex
J. Biol. Chem., January 13, 2006; 281(2): 927 - 933.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C.-H. Kim, W. C. Xiong, and L. Mei
Inhibition of MuSK Expression by CREB Interacting with a CRE-Like Element and MyoD
Mol. Cell. Biol., July 1, 2005; 25(13): 5329 - 5338.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Lu, K. A. Seta, and D. E. Millhorn
Novel Role for Cyclin-dependent Kinase 2 in Neuregulin-induced Acetylcholine Receptor {epsilon} Subunit Expression in Differentiated Myotubes
J. Biol. Chem., June 10, 2005; 280(23): 21731 - 21738.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Z. Gu, Q. Jiang, A. K. Y. Fu, N. Y. Ip, and Z. Yan
Regulation of NMDA Receptors by Neuregulin Signaling in Prefrontal Cortex
J. Neurosci., May 18, 2005; 25(20): 4974 - 4984.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. C. Trinidad and J. B. Cohen
Neuregulin Inhibits Acetylcholine Receptor Aggregation in Myotubes
J. Biol. Chem., July 23, 2004; 279(30): 31622 - 31628.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Canto, E. Suarez, J. M. Lizcano, E. Grino, P. R. Shepherd, L. G. D. Fryer, D. Carling, J. Bertran, M. Palacin, A. Zorzano, et al.
Neuregulin Signaling on Glucose Transport in Muscle Cells
J. Biol. Chem., March 26, 2004; 279(13): 12260 - 12268.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-H. Kim, W. C. Xiong, and L. Mei
Regulation of MuSK Expression by a Novel Signaling Pathway
J. Biol. Chem., October 3, 2003; 278(40): 38522 - 38527.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. Ma, Y. Z. Huang, G. M. Pitcher, J. G. Valtschanoff, Y. H. Ma, L. Y. Feng, B. Lu, W. C. Xiong, M. W. Salter, R. J. Weinberg, et al.
Ligand-Dependent Recruitment of the ErbB4 Signaling Complex into Neuronal Lipid Rafts
J. Neurosci., April 15, 2003; 23(8): 3164 - 3175.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Z. Huang, M. Zang, W. C. Xiong, Z. Luo, and L. Mei
Erbin Suppresses the MAP Kinase Pathway
J. Biol. Chem., January 3, 2003; 278(2): 1108 - 1114.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. R. Andrechek, W. R. Hardy, A. A. Girgis-Gabardo, R. L. S. Perry, R. Butler, F. L. Graham, R. C. Kahn, M. A. Rudnicki, and W. J. Muller
ErbB2 Is Required for Muscle Spindle and Myoblast Cell Survival
Mol. Cell. Biol., July 1, 2002; 22(13): 4714 - 4722.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. L. Smith, P. Mittaud, E. D. Prescott, C. Fuhrer, and S. J. Burden
Src, Fyn, and Yes Are Not Required for Neuromuscular Synapse Formation But Are Necessary for Stabilization of Agrin-Induced Clusters of Acetylcholine Receptors
J. Neurosci., May 1, 2001; 21(9): 3151 - 3160.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Tanowitz, J. Si, D.-H. Yu, G.-S. Feng, and L. Mei
Regulation of Neuregulin-Mediated Acetylcholine Receptor Synthesis by Protein Tyrosine Phosphatase SHP2
J. Neurosci., November 1, 1999; 19(21): 9426 - 9435.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Si, Q. Wang, and L. Mei
Essential Roles of c-JUN and c-JUN N-Terminal Kinase (JNK) in Neuregulin-Increased Expression of the Acetylcholine Receptor epsilon -Subunit
J. Neurosci., October 1, 1999; 19(19): 8498 - 8508.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
I. Alroy, L. Soussan, R. Seger, and Y. Yarden
Neu Differentiation Factor Stimulates Phosphorylation and Activation of the Sp1 Transcription Factor
Mol. Cell. Biol., March 1, 1999; 19(3): 1961 - 1972.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
P. A. Felts
Neuregulins in Schwann Cell Development
Neuroscientist, January 1, 1999; 5(1): 8 - 11.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
L. Fromm and S. J. Burden
Synapse-specific and neuregulin-induced transcription require an Ets site that binds GABPalpha /GABPbeta
Genes & Dev., October 1, 1998; 12(19): 3074 - 3083.
[Abstract] [Full Text]

Home page
 JCBHome page
T. Meier, F. Masciulli, C. Moore, F. Schoumacher, U. Eppenberger, A. J. Denzer, G. Jones, and H. R. Brenner
Agrin Can Mediate Acetylcholine Receptor Gene Expression in Muscle by Aggregation of Muscle-derived Neuregulins
J. Cell Biol., May 4, 1998; 141(3): 715 - 726.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
A. G. Engel, K. Ohno, H.-L. Wang, M. Milone, and S. M. Sine
REVIEW {blacksquare} : Molecular Basis of Congenital Myasthenic Syndromes: Mutations in the Acetylcholine Receptor
Neuroscientist, May 1, 1998; 4(3): 185 - 194.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. K. Sapru, S. K. Florance, C. Kirk, and D. Goldman
Identification of a neuregulin and protein-tyrosine phosphatase response element in the nicotinic acetylcholine receptor varepsilon  subunit gene: Regulatory role of an Ets transcription factor
PNAS, February 3, 1998; 95(3): 1289 - 1294.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. J. Burden
The formation of neuromuscular synapses
Genes & Dev., January 15, 1998; 12(2): 133 - 148.
[Full Text]

Home page
 J. Neurosci.Home page
M. Colledge and S. C. Froehner
Tyrosine Phosphorylation of Nicotinic Acetylcholine Receptor Mediates Grb2 Binding
J. Neurosci., July 1, 1997; 17(13): 5038 - 5045.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Si, D. S. Miller, and L. Mei
Identification of an Element Required for Acetylcholine Receptor-inducing Activity (ARIA)-induced Expression of the Acetylcholine Receptor epsilon Subunit Gene
J. Biol. Chem., April 18, 1997; 272(16): 10367 - 10371.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Z. Huang, Q. Wang, W. C. Xiong, and L. Mei
Erbin Is a Protein Concentrated at Postsynaptic Membranes That Interacts with PSD-95
J. Biol. Chem., May 25, 2001; 276(22): 19318 - 19326.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Tang, Z. Sun, and D. Goldman
CaM Kinase II-dependent Suppression of Nicotinic Acetylcholine Receptor delta -Subunit Promoter Activity
J. Biol. Chem., July 6, 2001; 276(28): 26057 - 26065.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Li and J. A. Loeb
Neuregulin-Heparan-sulfate Proteoglycan Interactions Produce Sustained erbB Receptor Activation Required for the Induction of Acetylcholine Receptors in Muscle
J. Biol. Chem., October 5, 2001; 276(41): 38068 - 38075.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.