HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Caplan, S. Articles by Baniyash, M. Search for Related Content PubMed PubMed Citation Articles by Caplan, S. Articles by Baniyash, M. Social Bookmarking                      What's this?

Volume 271, Number 34, Issue of August 23, 1996 pp. 20705-20712
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Normal T Cells Express Two T Cell Antigen Receptor Populations, One of Which Is Linked to the Cytoskeleton via  Chain and Displays a Unique Activation-dependent Phosphorylation Pattern

(Received for publication, March 5, 1996)

From The Lautenberg Center for General and Tumor Immunology, Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel

The TCR couples antigen recognition and the transmission of activation signals. We report the expression of two TCR populations on the surface of T lymphocytes, one of which is linked to the cytoskeleton via the  chain. We also demonstrate that assembly of the CD3 subunits with cytoskeleton-associated  is necessary for their maximal localization to the cytoskeleton. The potential significance of these two receptor forms is underscored by differences observed in non-activated T cells; while detergent-soluble phosphorylated  appears as a 21-kDa protein, phosphorylated cytoskeleton-associated  appears as a 16-kDa form. This dichotomous phosphorylation pattern is rigidly maintained following activation, although each of the receptor populations undergoes different activation-dependent modifications: 1) levels of soluble phosphorylated 21-kDa  are enhanced, while phosphorylated 16-kDa cytoskeleton-associated  exhibits little change; 2) soluble non-phosphorylated 16-kDa  translocates to the cytoskeleton; 3) activation-dependent ubiquitinated  forms localize to both fractions, albeit with different kinetics. We also show that the protein tyrosine kinase Lck undergoes activation-dependent modifications and translocates to the cytoskeleton. The phosphorylation profiles of the dichotomous TCR populations in both non-activated and activated lymphocytes suggest that each population could regulate distinct cellular functions, possibly by select intermolecular associations.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. Varma TCR Triggering by the pMHC Complex: Valency, Affinity, and Dynamics Sci. Signal., May 13, 2008; 1(19): pe21 - pe21. [Abstract] [Full Text] [PDF]

				T. L. Lysechko and H. L. Ostergaard Differential Src Family Kinase Activity Requirements for CD3{zeta} Phosphorylation/ZAP70 Recruitment and CD3{epsilon} Phosphorylation J. Immunol., June 15, 2005; 174(12): 7807 - 7814. [Abstract] [Full Text] [PDF]

				T. A Lyubchenko, G. A Wurth, and A. Zweifach The actin cytoskeleton and cytotoxic T lymphocytes: evidence for multiple roles that could affect granule exocytosis-dependent target cell killing J. Physiol., March 15, 2003; 547(3): 835 - 847. [Abstract] [Full Text] [PDF]

				G. G. Garcia and R. A. Miller Age-Dependent Defects in TCR-Triggered Cytoskeletal Rearrangement in CD4+ T Cells J. Immunol., November 1, 2002; 169(9): 5021 - 5027. [Abstract] [Full Text] [PDF]

				S. Krishnan, V. G. Warke, M. P. Nambiar, H. K. Wong, G. C. Tsokos, and D. L. Farber Generation and biochemical analysis of human effector CD4 T cells: alterations in tyrosine phosphorylation and loss of CD3{zeta} expression Blood, June 15, 2001; 97(12): 3851 - 3859. [Abstract] [Full Text] [PDF]

				C. Krawczyk and J. M. Penninger Molecular motors involved in T cell receptor clusterings J. Leukoc. Biol., March 1, 2001; 69(3): 317 - 330. [Abstract] [Full Text]

				C. Arrieumerlou, C. Randriamampita, G. Bismuth, and A. Trautmann Rac Is Involved in Early TCR Signaling J. Immunol., September 15, 2000; 165(6): 3182 - 3189. [Abstract] [Full Text] [PDF]

				N. Bronstein-Sitton, L. Wang, L. Cohen, and M. Baniyash Expression of the T Cell Antigen Receptor zeta Chain following Activation Is Controlled at Distinct Checkpoints. IMPLICATIONS FOR CELL SURFACE RECEPTOR DOWN-MODULATION AND RE-EXPRESSION J. Biol. Chem., August 13, 1999; 274(33): 23659 - 23665. [Abstract] [Full Text] [PDF]

				A. E. Annenkov, S. P. Moyes, Z. Eshhar, R. A. Mageed, and Y. Chernajovsky Loss of Original Antigenic Specificity in T Cell Hybridomas Transduced with a Chimeric Receptor Containing Single-Chain Fv of an Anti-Collagen Antibody and Fc{epsilon}RI-Signaling {gamma} Subunit J. Immunol., December 15, 1998; 161(12): 6604 - 6613. [Abstract] [Full Text] [PDF]

				M. M. Rozdzial, C. M. Pleiman, J. C. Cambier, and T. H. Finkel3 pp56Lck Mediates TCR {zeta}-Chain Binding to the Microfilament Cytoskeleton J. Immunol., November 15, 1998; 161(10): 5491 - 5499. [Abstract] [Full Text] [PDF]

				D. G. Woodside, D. K. Wooten, and B. W. McIntyre Adenosine Diphosphate (ADP)-Ribosylation of the Guanosine Triphosphatase (GTPase) Rho in Resting Peripheral Blood Human T Lymphocytes Results in Pseudopodial Extension and the Inhibition of  T Cell Activation J. Exp. Med., October 5, 1998; 188(7): 1211 - 1221. [Abstract] [Full Text] [PDF]

				N. N. Berg, L. G. Puente, W. Dawicki, and H. L. Ostergaard Sustained TCR Signaling Is Required for Mitogen-Activated Protein Kinase Activation and Degranulation by Cytotoxic T Lymphocytes J. Immunol., September 15, 1998; 161(6): 2919 - 2924. [Abstract] [Full Text] [PDF]