HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marie-Cardine, A. Articles by Maridonneau-Parini, I. Search for Related Content PubMed PubMed Citation Articles by Marie-Cardine, A. Articles by Maridonneau-Parini, I. Social Bookmarking                      What's this?

Volume 271, Number 34, Issue of August 23, 1996 pp. 20734-20739
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Recruitment of Activated p56^lck on Endosomes of CD2-triggered T Cells, Colocalization with ZAP-70

(Received for publication, December 26, 1995, and in revised form, May 22, 1996)

Anne Marie-Cardine , Siegmund Fischer , Jean-Pierre Gorvel ^¶ and Isabelle Maridonneau-Parini

From  INSERM U332, Institut Cochin de Génétique Moléculaire, 75014 Paris and ^¶ Centre d'Immunologie INSERM/CNRS, 13288 Marseille Luminy, France

We have previously established that upon CD2 activation of T cells, p56^lck showed a transient increase in its kinase activity and was partially internalized. Here we studied the possibility that p56^lck could retain its kinase activity in the endosomes of CD2-triggered cells. T cells were fractionated on a sucrose gradient, and the endosomal fraction was isolated. In CD2-triggered cells, part of Lck was internalized and presented a maximal kinase activity in the endosome-enriched fraction after 5 min, decreasing thereafter. In the endosomal fraction of activated cells, four tyrosine-phosphorylated proteins of apparent molecular masses of 30, 40, 56, and 70 kDa were detected. We demonstrated that the protein tyrosine kinase ZAP-70 was recruited to the endosomal fraction upon CD2 stimulation with kinetics similar to that of p56^lck, suggesting that recruitment of protein tyrosine kinases to endosomal vesicles could promote specific transduction signals at the intracellular level.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. B. Strasner, M. Natarajan, T. Doman, D. Key, A. August, and A. J. Henderson The Src Kinase Lck Facilitates Assembly of HIV-1 at the Plasma Membrane J. Immunol., September 1, 2008; 181(5): 3706 - 3713. [Abstract] [Full Text] [PDF]

				K. Singleton, N. Parvaze, K. R. Dama, K. S. Chen, P. Jennings, B. Purtic, M. D. Sjaastad, C. Gilpin, M. M. Davis, and C. Wulfing A Large T Cell Invagination with CD2 Enrichment Resets Receptor Engagement in the Immunological Synapse J. Immunol., October 1, 2006; 177(7): 4402 - 4413. [Abstract] [Full Text] [PDF]

				N. Blanchard, D. Lankar, F. Faure, A. Regnault, C. Dumont, G. Raposo, and C. Hivroz TCR Activation of Human T Cells Induces the Production of Exosomes Bearing the TCR/CD3/{zeta} Complex J. Immunol., April 1, 2002; 168(7): 3235 - 3241. [Abstract] [Full Text] [PDF]

				M. P. Martelli, J. Boomer, M. Bu, and B. E. Bierer T Cell Regulation of p62dok (Dok1) Association with Crk-L J. Biol. Chem., November 30, 2001; 276(49): 45654 - 45661. [Abstract] [Full Text] [PDF]

				H. Lin, M. P. Martelli, and B. E. Bierer The involvement of the proto-oncogene p120 c-Cbl and ZAP-70 in CD2-mediated T cell activation Int. Immunol., January 1, 2001; 13(1): 13 - 22. [Abstract] [Full Text] [PDF]

				M. P. Martelli, H. Lin, W. Zhang, L. E. Samelson, and B. E. Bierer Signaling via LAT (linker for T-cell activation) and Syk/ZAP70 is required for ERK activation and NFAT transcriptional activation following CD2 stimulation Blood, September 15, 2000; 96(6): 2181 - 2190. [Abstract] [Full Text] [PDF]

				J.-G. Nemorin and P. Duplay Evidence That Lck-mediated Phosphorylation of p56dok and p62dok May Play a Role in CD2 Signaling J. Biol. Chem., May 5, 2000; 275(19): 14590 - 14597. [Abstract] [Full Text] [PDF]

				W. J. Kivens, S. W. Hunt III, J. L. Mobley, T. Zell, C. L. Dell, B. E. Bierer, and Y. Shimizu Identification of a Proline-Rich Sequence in the CD2 Cytoplasmic Domain Critical for Regulation of Integrin-Mediated Adhesion and Activation of Phosphoinositide 3-Kinase Mol. Cell. Biol., September 1, 1998; 18(9): 5291 - 5307. [Abstract] [Full Text]

				H. Welch and I. Maridonneau-Parini Hck Is Activated by Opsonized Zymosan and A23187 in Distinct Subcellular Fractions of Human Granulocytes J. Biol. Chem., January 3, 1997; 272(1): 102 - 109. [Abstract] [Full Text] [PDF]

				P. Peyron, I. Maridonneau-Parini, and T. Stegmann Fusion of Human Neutrophil Phagosomes with Lysosomes in Vitro. INVOLVEMENT OF TYROSINE KINASES OF THE Src FAMILY AND INHIBITION BY MYCOBACTERIA J. Biol. Chem., September 14, 2001; 276(38): 35512 - 35517. [Abstract] [Full Text] [PDF]