JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pei, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pei, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 271, Number 34, Issue of August 23, 1996 pp. 20879-20884
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

Identification of a Negative Glucocorticoid Response Element in the Rat Type 1 Vasoactive Intestinal Polypeptide Receptor Gene

(Received for publication, January 18, 1996, and in revised form, May 16, 1996)

Lin Pei

From the Division of Endocrinology, Cedars-Sinai Research Institute, UCLA School of Medicine, Los Angeles, California 90048

Glucocorticoids play important roles in lung development and function by modulating the expression of a variety of genes. The type 1 vasoactive intestinal polypeptide (VIP) receptor gene is highly expressed in the lung where it mediates VIP physiological functions. In this study, the effect of glucocorticoid on VIP receptor gene expression was examined. Dexamethasone (100 nM) suppresses endogenous VIP receptor mRNA expression in cultured lung cells. Transient transfection of lung cells with fusion constructs containing various portions of the VIP receptor 5'-flanking sequences linked to the luciferase reporter gene shows that 126 base pairs (bp) of the VIP receptor upstream sequences are sufficient to mediate transcriptional repression by glucocorticoid. DNase I footprinting demonstrates that purified glucocorticoid receptor (GR) binds to the VIP receptor promoter between -21 and -36 bp relative to the transcription start site. Point mutations within this binding site not only abolish GR binding and GR-mediated transcriptional repression of the VIP receptor gene, but basal transcription is also reduced to background levels. Co-transfection of GR expression vector and the VIP receptor GR binding site linked to the thymidine kinase promoter and luciferase shows that this sequence is sufficient to confer glucocorticoid-mediated transcriptional repression to a heterologous promoter. These results indicate that the VIP receptor gene contains a negative glucocorticoid response element between -21 and -36 bp that may act to regulate both basal and glucocorticoid-mediated expressions of the VIP receptor gene in lung cells.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Chen, T. J. Nickola, N. L. DiFronzo, A. M. Colberg-Poley, and M. C. Rose
Dexamethasone-Mediated Repression of MUC5AC Gene Expression in Human Lung Epithelial Cells
Am. J. Respir. Cell Mol. Biol., March 1, 2006; 34(3): 338 - 347.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
B. M. Necela and J. A. Cidlowski
Mechanisms of Glucocorticoid Receptor Action in Noninflammatory and Inflammatory Cells
Proceedings of the ATS, November 1, 2004; 1(3): 239 - 246.
[Full Text] [PDF]

Home page
 Endocr. Rev.Home page
K. De Bosscher, W. Vanden Berghe, and G. Haegeman
The Interplay between the Glucocorticoid Receptor and Nuclear Factor-{kappa}B or Activator Protein-1: Molecular Mechanisms for Gene Repression
Endocr. Rev., August 1, 2003; 24(4): 488 - 522.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
S. Greenstein, K. Ghias, N. L. Krett, and S. T. Rosen
Mechanisms of Glucocorticoid-mediated Apoptosis in Hematological Malignancies
Clin. Cancer Res., June 1, 2002; 8(6): 1681 - 1694.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. A. Wadekar, D. Li, S. Periyasamy, and E. R. Sanchez
Inhibition of Heat Shock Transcription Factor by GR
Mol. Endocrinol., August 1, 2001; 15(8): 1396 - 1410.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
W. Zhu, L. Song, H. Zhang, L. Matoney, E. LeCluyse, and B. Yan
Dexamethasone Differentially Regulates Expression of Carboxylesterase Genes in Humans and Rats
Drug Metab. Dispos., February 1, 2000; 28(2): 186 - 191.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
L. Pei
Phosphorylation Modulates the Function of the Vasoactive Intestinal Polypeptide Receptor Transcriptional Repressor Protein
J. Biol. Chem., January 14, 2000; 275(2): 1176 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
H. Ren and G. L. Stiles
Dexamethasone Stimulates Human A1 Adenosine Receptor (A1AR) Gene Expression through Multiple Regulatory Sites in Promoter B
Mol. Pharmacol., February 1, 1999; 55(2): 309 - 316.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N. Subramaniam, W. Cairns, and S. Okret
Glucocorticoids Repress Transcription from a Negative Glucocorticoid Response Element Recognized by Two Homeodomain-containing Proteins, Pbx and Oct-1
J. Biol. Chem., September 4, 1998; 273(36): 23567 - 23574.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Pei
Molecular Cloning of a Novel Transcriptional Repressor Protein of the Rat Type 1 Vasoactive Intestinal Peptide Receptor Gene
J. Biol. Chem., July 31, 1998; 273(31): 19902 - 19908.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Meyer, J. Carlstedt-Duke, and D. B. Starr
A Weak TATA Box Is a Prerequisite for Glucocorticoid-dependent Repression of the Osteocalcin Gene
J. Biol. Chem., December 5, 1997; 272(49): 30709 - 30714.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Meyer, D. B. Starr, and J. Carlstedt-Duke
The Rat Glucocorticoid Receptor Mutant K461A Differentiates between Two Different Mechanisms of Transrepression
J. Biol. Chem., August 22, 1997; 272(34): 21090 - 21095.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.