HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhu, A. Articles by Kuziora, M. A. Search for Related Content PubMed PubMed Citation Articles by Zhu, A. Articles by Kuziora, M. A. Social Bookmarking                      What's this?

Volume 271, Number 35, Issue of August 30, 1996 pp. 20993-20996
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

COMMUNICATION:
Homeodomain Interaction with the  Subunit of the General Transcription Factor TFIIE

(Received for publication, June 7, 1996, and in revised form, June 27, 1996)

From the Department of Biological Sciences, University of Pittsburgh, A234 Langley Hall, Pittsburgh, Pennsylvania 15260

Homeodomain-containing proteins play a crucial role as transcriptional regulators in the process of cell differentiation. The homeodomain performs a dual function in this regard, acting as a DNA binding domain and participating in protein-protein interactions that enhance DNA binding specificity or regulatory activity. Here we describe a homeodomain class-specific interaction with the  subunit of the general transcription factor TFIIE. We show that the Antennapedia and Abdominal-B homeodomains bind to TFIIE, but the even-skipped homeodomain does not. Using a two-hybrid assay performed in cultured cells, we demonstrate that the homeodomain-TFIIE interaction occurs in vivo. The Abdominal-B homeodomain is shown to activate transcription in vitro, and this activation can be blocked with anti-TFIIE antibody without affecting basal transcription levels. Together with published data demonstrating an interaction between proteins containing even-skipped class homeodomains and the TATA-binding protein (Um, M., Li, C., and Manley, J. L. (1995) Mol. Cell. Biol. 15, 5007-5016; Zhang, H., Catron, K. M., and Abate-Shen, C. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 1764-1769), these results suggest various homeodomain containing proteins interact with different general transcription factors, an observation that may have important implications for transcriptional regulation.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. Shi, X. Fan, A. Sevilimedu, and J. T. Lis RNA aptamers directed to discrete functional sites on a single protein structural domain PNAS, March 6, 2007; 104(10): 3742 - 3746. [Abstract] [Full Text] [PDF]

				S. J. Zanton and B. F. Pugh Full and partial genome-wide assembly and disassembly of the yeast transcription machinery in response to heat shock. Genes & Dev., August 15, 2006; 20(16): 2250 - 2265. [Abstract] [Full Text] [PDF]

				S. E. Bondos, X.-X. Tan, and K. S. Matthews Physical and Genetic Interactions Link Hox Function with Diverse Transcription Factors and Cell Signaling Proteins Mol. Cell. Proteomics, May 1, 2006; 5(5): 824 - 834. [Abstract] [Full Text] [PDF]

				S. E. Bondos, D. J. Catanese Jr., X.-X. Tan, A. Bicknell, L. Li, and K. S. Matthews Hox Transcription Factor Ultrabithorax Ib Physically and Genetically Interacts with Disconnected Interacting Protein 1, a Double-stranded RNA-binding Protein J. Biol. Chem., June 18, 2004; 279(25): 26433 - 26444. [Abstract] [Full Text] [PDF]

				M. A. Tijms and E. J. Snijder Equine arteritis virus non-structural protein 1, an essential factor for viral subgenomic mRNA synthesis, interacts with the cellular transcription co-factor p100 J. Gen. Virol., September 1, 2003; 84(9): 2317 - 2322. [Abstract] [Full Text] [PDF]

				S. J. Tang, T.-C. Suen, R. R. McInnes, and M. Buchwald Association of the TLX-2 Homeodomain and 14-3-3eta Signaling Proteins J. Biol. Chem., September 25, 1998; 273(39): 25356 - 25363. [Abstract] [Full Text] [PDF]

				K. Yokomori, C. P. Verrijzer, and R. Tjian An interplay between TATA box-binding protein and transcription factors IIE and IIA modulates DNA binding and transcription PNAS, June 9, 1998; 95(12): 6722 - 6727. [Abstract] [Full Text] [PDF]

				M. Hampsey Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 465 - 503. [Abstract] [Full Text] [PDF]

				I. Mikkola, J.-A. Bruun, T. Holm, and T. Johansen Superactivation of Pax6-mediated Transactivation from Paired Domain-binding Sites by DNA-independent Recruitment of Different Homeodomain Proteins J. Biol. Chem., February 2, 2001; 276(6): 4109 - 4118. [Abstract] [Full Text] [PDF]