JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xu, L.
Right arrow Articles by Otero, A. d. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xu, L.
Right arrow Articles by Otero, A. d. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 271, Number 35, Issue of August 30, 1996 pp. 21120-21125
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

Participation of Nucleoside-diphosphate Kinase in Muscarinic K+ Channel Activation Does Not Involve GTP Formation

(Received for publication, May 23, 1996, and in revised form, June 12, 1996)

Lan Xu , Joseph Murphy and Angela de S. Otero

From the Department of Molecular Physiology and Biological Physics, University of Virginia Medical School, Charlottesville, Virginia 22908

Agonist-bound muscarinic receptors open atrial K+ channels through a GTP-dependent pathway mediated by the G protein Gk. However, nucleotides other than GTP are also able to support channel activity, even in the absence of agonists. This process was proposed to be mediated by nucleoside-diphosphate (NDP) kinase, which would transfer phosphate from nucleotide triphosphates to the GDP bound to Gk, producing Gk-GTP without the need for receptor-induced GDP-GTP exchange. We examined the effect of antibodies to NDP kinase on the ATP-supported activity of atrial muscarinic K+ channels and the corresponding GIRK1/CIR channels expressed in HEK 293 cells. Inhibitory antibodies reduced ATP-induced channel openings, but this effect displayed an absolute requirement for agonist and was also seen with antibodies that do not inhibit the enzyme. Both types of antibodies also reduced agonist-dependent channel activity in the presence of GTP, ruling out a role for NDP kinase in GDP rephosphorylation. Channel activity was not affected by the antibodies in preparations where ATP-induced muscarinic channels are not under tight receptor control, namely pertussis toxin-treated atrial patches and membranes from cells expressing KACh channel subunits. Thus, participation of NDP kinase in this pathway requires activated receptors and has a function distinct from phosphate transfer between nucleotides.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
B. C. Gallagher, K. A. Parrott, G. Szabo, and A. de S. Otero
Receptor activation regulates cortical, but not vesicular localization of NDP kinase
J. Cell Sci., August 1, 2003; 116(15): 3239 - 3250.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
B. K. Stringer, A. G. Cooper, and S. B. Shepard
Overexpression of the G-Protein Inwardly Rectifying Potassium Channel 1 (GIRK1) in Primary Breast Carcinomas Correlates with Axillary Lymph Node Metastasis
Cancer Res., January 1, 2001; 61(2): 582 - 588.
[Abstract] [Full Text]

Home page
 Physiol. Rev.Home page
E. Carmeliet
Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias
Physiol Rev, July 1, 1999; 79(3): 917 - 1017.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. J. Marshall, R. Muimo, C. E. Riemen, and A. Mehta
Na+ and K+ regulate the phosphorylation state of nucleoside diphosphate kinase in human airway epithelium
Am J Physiol Cell Physiol, January 1, 1999; 276(1): C109 - C119.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. S. Otero, L. Xu, Y. Ni, and G. Szabo
Receptor-independent Activation of Atrial Muscarinic Potassium Channels in the Absence of Nucleotides
J. Biol. Chem., October 30, 1998; 273(44): 28868 - 28872.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Pleumsamran, M. L. Wolak, and D. Kim
Inhibition of ATP-induced increase in muscarinic K+ current by trypsin, alkaline pH, and anions
Am J Physiol Heart Circ Physiol, September 1, 1998; 275(3): H751 - H759.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Sorota, M. Chlenov, X.-Y. Du, and M. Kagan
ATP-Dependent Activation of the Atrial Acetylcholine-Induced K+ Channel Does Not Require Nucleoside Diphosphate Kinase Activity
Circ. Res., May 19, 1998; 82(9): 971 - 979.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. d. S. Otero
Copurification of Vimentin, Energy Metabolism Enzymes, and a MER5 Homolog with Nucleoside Diphosphate Kinase. IDENTIFICATION OF TISSUE-SPECIFIC INTERACTIONS
J. Biol. Chem., June 6, 1997; 272(23): 14690 - 14694.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
N. Abi-Gerges, G. Szabo, A. S. Otero, R. Fischmeister, and P.-F. Mery
NO donors potentiate the beta-adrenergic stimulation of ICa,L and the muscarinic activation of IK,ACh in rat cardiac myocytes
J. Physiol., April 15, 2002; 540(2): 411 - 424.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.