HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tang, C.-M. Articles by Seto, E. Search for Related Content PubMed PubMed Citation Articles by Tang, C.-M. Articles by Seto, E. Social Bookmarking                      What's this?

Volume 271, Number 35, Issue of August 30, 1996 pp. 21637-21644
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Replication Protein A Is a Component of a Complex That Binds the Human Metallothionein IIA Gene Transcription Start Site

(Received for publication, November 28, 1995, and in revised form, April 7, 1996)

Chih-Min Tang ^§ , Alan E. Tomkinson ^§ , William S. Lane ^¶ , Marc S. Wold and Edward Seto ^§

From the  Moffitt Cancer Center & Research Institute, University of South Florida, Tampa, Florida, 33612, the ^§ Center for Molecular Medicine/Institute of Biotechnology, Department of Cellular & Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78245-3207, the ^¶ Harvard Microchemistry Facility, Harvard University, Cambridge, Massachusetts 02138, and the  Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242

Previous studies revealed that sequences surrounding the initiation sites in many mammalian and viral gene promoters, called initiator (Inr) elements, may be essential for promoter strength and for determining the actual transcription start sites. DNA sequences in the vicinity of the human metallothionein IIA (hMTIIA) gene transcription start site share homology with some of the previously identified Inr elements. However, in the present study we have found by in vitro transcription assays that the hMTIIA promoter does not contain a typical Inr. Electrophoretic mobility shift assays identified several DNA-protein complexes at the hMTIIA gene transcription start site. A partially purified protein fraction containing replication protein A (RPA) binds to the hMTIIA gene transcription start site and represses transcription from the hMTIIA promoter in vitro. In addition, overexpression of the human 70-kDa RPA-1 protein represses transcription of a reporter gene controlled by the hMTIIA promoter in vivo. These findings suggest that hMTIIA transcription initiation is controlled by a mechanism different from most mammalian and viral promoters and that the previously identified RPA may also be involved in transcription regulation.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. E. Sukhodolets, A. B. Hickman, S. K. Agarwal, M. V. Sukhodolets, V. H. Obungu, E. A. Novotny, J. S. Crabtree, S. C. Chandrasekharappa, F. S. Collins, A. M. Spiegel, et al. The 32-Kilodalton Subunit of Replication Protein A Interacts with Menin, the Product of the MEN1 Tumor Suppressor Gene Mol. Cell. Biol., January 15, 2003; 23(2): 493 - 509. [Abstract] [Full Text] [PDF]

				G. G. Oakley, L. I. Loberg, J. Yao, M. A. Risinger, R. L. Yunker, M. Zernik-Kobak, K. K. Khanna, M. F. Lavin, M. P. Carty, and K. Dixon UV-induced Hyperphosphorylation of Replication Protein A Depends on DNA Replication and Expression of ATM Protein Mol. Biol. Cell, May 1, 2001; 12(5): 1199 - 1213. [Abstract] [Full Text]

				C.-M. Tang, J. Westling, and E. Seto trans Repression of the Human Metallothionein IIA Gene Promoter by PZ120, a Novel 120-Kilodalton Zinc Finger Protein Mol. Cell. Biol., January 1, 1999; 19(1): 680 - 689. [Abstract] [Full Text] [PDF]

				E. van der Knaap, S. Jagoueix, and H. Kende Expression of an ortholog of replication protein A1 (RPA1) is induced by gibberellin in deepwater rice PNAS, September 2, 1997; 94(18): 9979 - 9983. [Abstract] [Full Text] [PDF]