| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, January 16, 1996, and in revised form, May 9, 1996)
From the Falk Cardiovascular Research Center, Stanford University
School of Medicine, Stanford, California 94305-5246
The present study tests the hypothesis that the
unique intracellular third loop domain of angiotensin II type-2 (AT2)
receptor is essential for the subsequent intracellular signaling and
plays an important role in mediating receptor function. Synthetic
intracellular third loop peptide of the AT2 receptor (AT2-3LP, 22 amino acids) and control peptide consisting of the same amino acid
composition in random sequence were delivered into adult rat aortic
vascular smooth muscle cells by cationic liposome-mediated
transfection. Successful intracellular peptide delivery was confirmed
by microscopic localization of the fluorescein-labeled AT2-3LP within
the cells and also by co-immunoprecipitation of the
125I-labeled 3LP complexed with Gi protein
using anti-Gi
antibody. The AT2-3LP-transfected cells
showed reduction of serum-stimulated DNA synthesis and cell
proliferation as well as a decrease in mitogen-activated protein kinase
activity, simulating the effects of AT2 receptor stimulation. The
antagonistic effect of the AT2-3LP on mitogen-activated protein kinase
activity and DNA synthesis were reversed by pertussis toxin and sodium
orthovanadate. Thus, our data suggest that the intracellular third loop
domain of the AT2 receptor is closely linked with the cellular
signaling pathways of vascular smooth muscle cells in which
Gi and protein-phosphotyrosine phosphatase are involved,
resulting in the alteration of mitogen-activated protein kinase
activity and in growth inhibition.
This article has been cited by other articles:
|
|
 |
|
  M. Mogi, M. Iwai, and M. Horiuchi Emerging Concepts of Regulation of Angiotensin II Receptors: New Players and Targets for Traditional Receptors Arterioscler. Thromb. Vasc. Biol., December 1, 2007; 27(12): 2532 - 2539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tsuda, M. Iwai, J.-M. Li, H.-S. Li, L.-J. Min, A. Ide, M. Okumura, J. Suzuki, M. Mogi, H. Suzuki, et al. Inhibitory Effects of AT1 Receptor Blocker, Olmesartan, and Estrogen on Atherosclerosis Via Anti-Oxidative Stress Hypertension, April 1, 2005; 45(4): 545 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Wruck, H. Funke-Kaiser, T. Pufe, H. Kusserow, M. Menk, J. H. Schefe, M. L. Kruse, M. Stoll, and T. Unger Regulation of Transport of the Angiotensin AT2 Receptor by a Novel Membrane-Associated Golgi Protein Arterioscler. Thromb. Vasc. Biol., January 1, 2005; 25(1): 57 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Granier, S. Terrillon, R. Pascal, H. Demene, M. Bouvier, G. Guillon, and C. Mendre A Cyclic Peptide Mimicking the Third Intracellular Loop of the V2 Vasopressin Receptor Inhibits Signaling through Its Interaction with Receptor Dimer and G Protein J. Biol. Chem., December 3, 2004; 279(49): 50904 - 50914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kumar, V. Menon, W. R. Ford, A. S. Clanachan, and B. I. Jugdutt Effect of Angiotensin II lype 2 Receptor Blockade on Activation of Mitogen-Activated Protein Kinases after Ischemia-Reperfusion in Isolated Working Rat Hearts Journal of Cardiovascular Pharmacology and Therapeutics, December 1, 2003; 8(4): 285 - 296. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Thai, J. Wollmuth, S. Goldman, and M. Gaballa Angiotensin Subtype 1 Receptor (AT1) Blockade Improves Vasorelaxation in Heart Failure by Up-Regulation of Endothelial Nitric-Oxide Synthase via Activation of the AT2 Receptor J. Pharmacol. Exp. Ther., December 1, 2003; 307(3): 1171 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Horiuchi, T.-X. Cui, Z. Li, J.-M. Li, H. Nakagami, and M. Iwai Fluvastatin Enhances the Inhibitory Effects of a Selective Angiotensin II Type 1 Receptor Blocker, Valsartan, on Vascular Neointimal Formation Circulation, January 7, 2003; 107(1): 106 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-H. Feng, Y. Sun, and J. G. Douglas Gbeta gamma -independent constitutive association of Galpha s with SHP-1 and angiotensin II receptor AT2 is essential in AT2-mediated ITIM-independent activation of SHP-1 PNAS, September 17, 2002; 99(19): 12049 - 12054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takeda-Matsubara, H. Nakagami, M. Iwai, T.-X. Cui, T. Shiuchi, M. Akishita, C. Nahmias, M. Ito, and M. Horiuchi Estrogen Activates Phosphatases and Antagonizes Growth-Promoting Effect of Angiotensin II Hypertension, January 1, 2002; 39(1): 41 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Brede, K. Hadamek, L. Meinel, F. Wiesmann, J. Peters, S. Engelhardt, A. Simm, A. Haase, M. J. Lohse, and L. Hein Vascular Hypertrophy and Increased P70S6 Kinase in Mice Lacking the Angiotensin II AT2 Receptor Circulation, November 20, 2001; 104(21): 2602 - 2607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hines, J. N. Heerding, S. J. Fluharty, and D. K. Yee Identification of Angiotensin II Type 2 (AT2) Receptor Domains Mediating High-Affinity CGP 42112A Binding and Receptor Activation J. Pharmacol. Exp. Ther., August 1, 2001; 298(2): 665 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-X. Cui, H. Nakagami, M. Iwai, Y. Takeda, T. Shiuchi, L. Daviet, C. Nahmias, and M. Horiuchi Pivotal role of tyrosine phosphatase SHP-1 in AT2 receptor-mediated apoptosis in rat fetal vascular smooth muscle cell Cardiovasc Res, March 1, 2001; 49(4): 863 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hansen, G. Servant, T. J. Baranski, T. Fujita, T. Iiri, and S. P. Sheikh Functional Reconstitution of the Angiotensin II Type 2 Receptor and Gi Activation Circ. Res., October 27, 2000; 87(9): 753 - 759. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. de Gasparo, K. J. Catt, T. Inagami, J. W. Wright, and Th. Unger International Union of Pharmacology. XXIII. The Angiotensin II Receptors Pharmacol. Rev., September 1, 2000; 52(3): 415 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Andreis, A. Markowska, H. C. Champion, G. Mazzocchi, L. K. Malendowicz, and G. G. Nussdorfer Adrenomedullin Enhances Cell Proliferation and Deoxyribonucleic Acid Synthesis in Rat Adrenal Zona Glomerulosa: Receptor Subtype Involved and Signaling Mechanism Endocrinology, June 1, 2000; 141(6): 2098 - 2104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sun, J. Zhang, J. Q. Zhang, and F. J. A. Ramires Local Angiotensin II and Transforming Growth Factor-{beta}1 in Renal Fibrosis of Rats Hypertension, May 1, 2000; 35(5): 1078 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kim and H. Iwao Molecular and Cellular Mechanisms of Angiotensin II-Mediated Cardiovascular and Renal Diseases Pharmacol. Rev., March 1, 2000; 52(1): 11 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gallinat, S. Busche, M. K. Raizada, and C. Sumners The angiotensin II type 2 receptor: an enigma with multiple variations Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E357 - E374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horiuchi, W. Hayashida, M. Akishita, S. Yamada, J. Y. A. Lehtonen, K. Tamura, L. Daviet, Y. E. Chen, M. Hamai, T.-X. Cui, et al. Interferon-{gamma} Induces AT2 Receptor Expression in Fibroblasts by Jak/STAT Pathway and Interferon Regulatory Factor-1 Circ. Res., February 4, 2000; 86(2): 233 - 240. [Abstract] [Full Text]
|
|
|
|
|
|
R. M. Carey, Z.-Q. Wang, and H. M. Siragy Role of the Angiotensin Type 2 Receptor in the Regulation of Blood Pressure and Renal Function Hypertension, January 1, 2000; 35(1): 155 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. A. Lehtonen, L. Daviet, C. Nahmias, M. Horiuchi, and V. J. Dzau Analysis of Functional Domains of Angiotensin II Type 2 Receptor Involved in Apoptosis Mol. Endocrinol., July 1, 1999; 13(7): 1051 - 1060. [Abstract] [Full Text]
|
|
|
|
|
|
H. Yamada, M. Akishita, M. Ito, K. Tamura, L. Daviet, J. Y. A. Lehtonen, V. J. Dzau, and M. Horiuchi AT2 Receptor and Vascular Smooth Muscle Cell Differentiation in Vascular Development Hypertension, June 1, 1999; 33(6): 1414 - 1419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horiuchi, W. Hayashida, M. Akishita, K. Tamura, L. Daviet, J. Y. A. Lehtonen, and V. J. Dzau Stimulation of Different Subtypes of Angiotensin II Receptors, AT1 and AT2 Receptors, Regulates STAT Activation by Negative Crosstalk Circ. Res., April 30, 1999; 84(8): 876 - 882. [Abstract] [Full Text]
|
|
|
|
|
|
G. Iaccarino, L. A. Smithwick, R. J. Lefkowitz, and W. J. Koch Targeting Gbeta gamma signaling in arterial vascular smooth muscle proliferation: A novel strategy to limit restenosis PNAS, March 30, 1999; 96(7): 3945 - 3950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Peterson, H. Ju, J. Hao, M. Panagia, D. C. Chapman, and I. M.C. Dixon Expression of Gi-2{alpha} and Gs{alpha} in myofibroblasts localized to the infarct scar in heart failure due to myocardial infarction Cardiovasc Res, March 1, 1999; 41(3): 575 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horiuchi, M. Akishita, and V. J. Dzau Recent Progress in Angiotensin II Type 2 Receptor Research in the Cardiovascular System Hypertension, February 1, 1999; 33(2): 613 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Franzoni, G. Nicastro, T. A. Pertinhez, E. Oliveira, C. R. Nakaie, A. C. M. Paiva, S. Schreier, and A. Spisni Structure of Two Fragments of the Third Cytoplasmic Loop of the Rat Angiotensin II AT1A Receptor. IMPLICATIONS WITH RESPECT TO RECEPTOR ACTIVATION AND G-PROTEIN SELECTION AND COUPLING J. Biol. Chem., January 1, 1999; 274(1): 227 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Matsubara Pathophysiological Role of Angiotensin II Type 2 Receptor in Cardiovascular and Renal Diseases Circ. Res., December 14, 1998; 83(12): 1182 - 1191. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. A. Fischer, K. Singh, D. S. O'Hara, D. M. Kaye, and R. A. Kelly Role of AT1 and AT2 receptors in regulation of MAPKs and MKP-1 by ANG II in adult cardiac myocytes Am J Physiol Heart Circ Physiol, September 1, 1998; 275(3): H906 - H916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L-D. Wang, M. Hoeltzel, I. Gantz, R. Hunter, and J. Del Valle Characterization of the Histamine H2 Receptor Structural Components Involved in Dual Signaling J. Pharmacol. Exp. Ther., May 1, 1998; 285(2): 573 - 578. [Abstract] [Full Text]
|
|
|
|
|
|
S. Conchon, M.-B. Barrault, S. Miserey, P. Corvol, and E. Clauser The C-terminal Third Intracellular Loop of the Rat AT1A Angiotensin Receptor Plays a Key Role in G Protein Coupling Specificity and Transduction of the Mitogenic Signal J. Biol. Chem., October 10, 1997; 272(41): 25566 - 25572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horiuchi, W. Hayashida, T. Kambe, T. Yamada, and V. J. Dzau Angiotensin Type 2 Receptor Dephosphorylates Bcl-2 by Activating Mitogen-activated Protein Kinase Phosphatase-1 and Induces Apoptosis J. Biol. Chem., July 25, 1997; 272(30): 19022 - 19026. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
