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(Received for publication, April 2, 1996, and in revised form, June 6, 1996)
From the According to their role in translation, tRNAs
specifically interact either with elongation factor Tu (EFTu) or with
initiation factor 2 (IF2). We here describe the effects of
overproducing EFTu and IF2 on the elongator versus
initiator activities of various mutant tRNAMet species
in vivo. The data obtained indicate that the selection of a
tRNA through one or the other pathway of translation depends on the
relative amounts of the translational factors. A moderate
overexpression of EFTu is enough to lead to a misappropriation of
initiator tRNA in the elongation process, whereas overproduced IF2
allows the initiation of translation to occur with unformylated tRNA
species. In addition, we report that a strain devoid of formylase
activity can be cured by the overproduction of
tRNAMetf. The present
study brings additional evidence for the importance of formylation in
defining tRNAMetf
initiator identity, as well as a possible explanation for the residual
growth of bacterial strains lacking a functional formylase gene such as
observed in Guillon, J. M., Mechulam, Y., Schmitter, J.-M., Blanquet,
S., and Fayat, G. (1992) J. Bacteriol. 174, 4294-4301.
Volume 271, Number 37,
Issue of September 13, 1996
pp. 22321-22325
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
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Laboratoire de Biochimie, URA CNRS 1970, Ecole Polytechnique, F91128 Palaiseau cedex, France and the
§ Institut de Biologie Physico-Chimique, URA CNRS 1139, 13 rue Pierre et Marie Curie, F75005 Paris, France
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