HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guillon, J.-M. Articles by Blanquet, S. Search for Related Content PubMed PubMed Citation Articles by Guillon, J.-M. Articles by Blanquet, S. Social Bookmarking                      What's this?

Volume 271, Number 37, Issue of September 13, 1996 pp. 22321-22325
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Interplay of Methionine tRNAs with Translation Elongation Factor Tu and Translation Initiation Factor 2 in Escherichia coli

(Received for publication, April 2, 1996, and in revised form, June 6, 1996)

Jean-Michel Guillon , Senta Heiss , Julie Soutourina , Yves Mechulam , Soumaya Laalami ^§ , Marianne Grunberg-Manago ^§ and Sylvain Blanquet

From the  Laboratoire de Biochimie, URA CNRS 1970, Ecole Polytechnique, F91128 Palaiseau cedex, France and the ^§ Institut de Biologie Physico-Chimique, URA CNRS 1139, 13 rue Pierre et Marie Curie, F75005 Paris, France

According to their role in translation, tRNAs specifically interact either with elongation factor Tu (EFTu) or with initiation factor 2 (IF2). We here describe the effects of overproducing EFTu and IF2 on the elongator versus initiator activities of various mutant tRNA^Met species in vivo. The data obtained indicate that the selection of a tRNA through one or the other pathway of translation depends on the relative amounts of the translational factors. A moderate overexpression of EFTu is enough to lead to a misappropriation of initiator tRNA in the elongation process, whereas overproduced IF2 allows the initiation of translation to occur with unformylated tRNA species. In addition, we report that a strain devoid of formylase activity can be cured by the overproduction of tRNA^Met_f. The present study brings additional evidence for the importance of formylation in defining tRNA^Met_f initiator identity, as well as a possible explanation for the residual growth of bacterial strains lacking a functional formylase gene such as observed in Guillon, J. M., Mechulam, Y., Schmitter, J.-M., Blanquet, S., and Fayat, G. (1992) J. Bacteriol. 174, 4294-4301.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. I. Nilsson, A. Zorzet, A. Kanth, S. Dahlstrom, O. G. Berg, and D. I. Andersson Reducing the fitness cost of antibiotic resistance by amplification of initiator tRNA genes PNAS, May 2, 2006; 103(18): 6976 - 6981. [Abstract] [Full Text] [PDF]

				L. D. Kapp, S. E. Kolitz, and J. R. Lorsch Yeast initiator tRNA identity elements cooperate to influence multiple steps of translation initiation RNA, May 1, 2006; 12(5): 751 - 764. [Abstract] [Full Text] [PDF]

				A. T. R. Vasconcelos, H. B. Ferreira, C. V. Bizarro, S. L. Bonatto, M. O. Carvalho, P. M. Pinto, D. F. Almeida, L. G. P. Almeida, R. Almeida, L. Alves-Filho, et al. Swine and Poultry Pathogens: the Complete Genome Sequences of Two Strains of Mycoplasma hyopneumoniae and a Strain of Mycoplasma synoviae J. Bacteriol., August 15, 2005; 187(16): 5568 - 5577. [Abstract] [Full Text] [PDF]

				G. Das, T. K. Dineshkumar, S. Thanedar, and U. Varshney Acquisition of a stable mutation in metY allows efficient initiation from an amber codon in Escherichia coli Microbiology, June 1, 2005; 151(6): 1741 - 1750. [Abstract] [Full Text] [PDF]

				B. S. Laursen, H. P. Sorensen, K. K. Mortensen, and H. U. Sperling-Petersen Initiation of Protein Synthesis in Bacteria Microbiol. Mol. Biol. Rev., March 1, 2005; 69(1): 101 - 123. [Abstract] [Full Text] [PDF]

				M. Steiner-Mosonyi, C. Creuzenet, R. A. B. Keates, B. R. Strub, and D. Mangroo The Pseudomonas aeruginosa Initiation Factor IF-2 Is Responsible for Formylation-independent Protein Initiation in P. aeruginosa J. Biol. Chem., December 10, 2004; 279(50): 52262 - 52269. [Abstract] [Full Text] [PDF]

				N. C. Martin Location alters tRNA identity: Trypanosoma brucei's cytosolic elongator tRNAMet is both the initiator and elongator in mitochondria PNAS, February 5, 2002; 99(3): 1110 - 1112. [Full Text] [PDF]

				D. T. Newton, C. Creuzenet, and D. Mangroo Formylation Is Not Essential for Initiation of Protein Synthesis in All Eubacteria J. Biol. Chem., August 6, 1999; 274(32): 22143 - 22146. [Abstract] [Full Text] [PDF]

				M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]

				S. Thanedar, N. V. Kumar, and U. Varshney The Fate of the Initiator tRNAs Is Sensitive to the Critical Balance between Interacting Proteins J. Biol. Chem., June 30, 2000; 275(27): 20361 - 20367. [Abstract] [Full Text] [PDF]