JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petrovic, N.
Right arrow Articles by Gross, K. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petrovic, N.
Right arrow Articles by Gross, K. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 271, Number 37, Issue of September 13, 1996 pp. 22499-22505
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

Role of Proximal Promoter Elements in Regulation of Renin Gene Transcription

(Received for publication, April 10, 1996, and in revised form, June 21, 1996)

Nenad Petrovic , Thomas A. Black , John R. Fabian , Colleen Kane , Craig A. Jones , John A. Loudon , J. Pablo Abonia , Curt D. Sigmund Dagger and Kenneth W. Gross

From the Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263 and the Dagger  Cardiovascular Diseases Division, Departments of Internal Medicine and Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242

Mouse As4.1 cells, obtained after transgene-targeted oncogenesis to induce neoplasia in renal renin-expressing cells, express high levels of renin mRNA from the endogenous Ren-1c gene. We have used these cells to characterize the role of the Ren-1c proximal promoter (+6 to -117) in the regulation of renin gene transcription. It was found that 4.1 kilobases (kb) of Ren-1c 5'-flanking sequence, in combination with the proximal promoter, are required for strong activation (~2 orders of magnitude over the basal level of the promoter alone) of the chloramphenicol acetyltransferase reporter in transfection assays. Within the 4.1-kb fragment, a 241-base pair region was identified that retains full activity in an orientation-independent manner in combination with the promoter. The resulting transcripts initiate at the normal renin start site. Electrophoretic mobility shift assays identified a sequence at approximately position -60 in the promoter region that binds nuclear proteins specific for renin-expressing As4.1 cells. Mutations in this sequence, which disrupt binding of nuclear protein(s), completely abolish activation of transcription by the 4.1-kb fragment. Activation of transcription by the 241-base pair enhancer was still observed, although it was diminished in magnitude (60-fold over the mutated promoter alone). We present a model derived from the current data that suggests that regulation of renin expression is achieved through cooperation of transcription factors binding at the proximal promoter element and a distal enhancer element to abrogate or override the effects of an intervening negative regulatory region.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
V. T. Todorov, M. Desch, T. Schubert, and A. Kurtz
The Pal3 Promoter Sequence Is Critical for the Regulation of Human Renin Gene Transcription by Peroxisome Proliferator-Activated Receptor-{gamma}
Endocrinology, September 1, 2008; 149(9): 4647 - 4657.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Zhou, E. T. Weatherford, X. Liu, E. Born, H. L. Keen, and C. D. Sigmund
Dysregulated human renin expression in transgenic mice carrying truncated genomic constructs: evidence supporting the presence of insulators at the renin locus
Am J Physiol Renal Physiol, September 1, 2008; 295(3): F642 - F653.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. A. Markus, C. Goy, D. J. Adams, F. J. Lovicu, and B. J. Morris
Renin Enhancer Is Crucial for Full Response in Renin Expression to an In Vivo Stimulus
Hypertension, November 1, 2007; 50(5): 933 - 938.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Yuan, W. Pan, J. Kong, W. Zheng, F. L. Szeto, K. E. Wong, R. Cohen, A. Klopot, Z. Zhang, and Y. C. Li
1,25-Dihydroxyvitamin D3 Suppresses Renin Gene Transcription by Blocking the Activity of the Cyclic AMP Response Element in the Renin Gene Promoter
J. Biol. Chem., October 12, 2007; 282(41): 29821 - 29830.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
N. Moore, P. Dicker, J. K. O'Brien, M. Stojanovic, R. M. Conroy, A. Treumann, E. T. O'Brien, D. Fitzgerald, D. Shields, and A. V. Stanton
Renin Gene Polymorphisms and Haplotypes, Blood Pressure, and Responses to Renin-Angiotensin System Inhibition
Hypertension, August 1, 2007; 50(2): 340 - 347.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. A. Itani, X. Liu, J. H. Pratt, and C. D. Sigmund
Functional Characterization of Polymorphisms in the Kidney Enhancer of the Human Renin Gene
Endocrinology, March 1, 2007; 148(3): 1424 - 1430.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X. Liu, Q. Shi, and C. D. Sigmund
Interleukin-1{beta} Attenuates Renin Gene Expression Via a Mitogen-Activated Protein Kinase Kinase-Extracellular Signal-Regulated Kinase and Signal Transducer and Activator of Transcription 3-Dependent Mechanism in As4.1 Cells
Endocrinology, December 1, 2006; 147(12): 6011 - 6018.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zhoux, D. R. Davis, and C. D. Sigmund
The Human Renin Kidney Enhancer Is Required to Maintain Base-line Renin Expression but Is Dispensable for Tissue-specific, Cell-specific, and Regulated Expression
J. Biol. Chem., November 17, 2006; 281(46): 35296 - 35304.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. J. Adams, G. A. Head, M. A. Markus, F. J. Lovicu, L. van der Weyden, F. Kontgen, M. J. Arends, S. Thiru, D. N. Mayorov, and B. J. Morris
Renin Enhancer Is Critical for Control of Renin Gene Expression and Cardiovascular Function
J. Biol. Chem., October 20, 2006; 281(42): 31753 - 31761.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
L. J. Mullins, M. A. Bailey, and J. J. Mullins
Hypertension, Kidney, and Transgenics: A Fresh Perspective
Physiol Rev, April 1, 2006; 86(2): 709 - 746.
[Abstract] [Full Text] [PDF]

Home page
 DNA ResHome page
A. A. Sharov, D. B. Dudekula, and M. S. H. Ko
CisView: A Browser and Database of cis-regulatory Modules Predicted in the Mouse Genome
DNA Res, January 1, 2006; 13(3): 123 - 134.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. T. Todorov, S. Volkl, J. Friedrich, L. A. Kunz-Schughart, T. Hehlgans, L. Vermeulen, G. Haegeman, M. L. Schmitz, and A. Kurtz
Role of CREB1 and NF{kappa}B-p65 in the Down-regulation of Renin Gene Expression by Tumor Necrosis Factor {alpha}
J. Biol. Chem., July 1, 2005; 280(26): 24356 - 24362.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Pan, S. T. Glenn, C. A. Jones, and K. W. Gross
Activation of the Rat Renin Promoter by HOXD10{middle dot}PBX1b{middle dot}PREP1, Ets-1, and the Intracellular Domain of Notch
J. Biol. Chem., May 27, 2005; 280(21): 20860 - 20866.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Pan, Y. Wang, C. A. Jones, S. T. Glenn, H. Baumann, and K. W. Gross
Enhancer-dependent inhibition of mouse renin transcription by inflammatory cytokines
Am J Physiol Renal Physiol, January 1, 2005; 288(1): F117 - F124.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
L. Pan and K. W. Gross
Transcriptional Regulation of Renin: An Update
Hypertension, January 1, 2005; 45(1): 3 - 8.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Pan, C. A. Jones, S. T. Glenn, and K. W. Gross
Identification of a novel region in the proximal promoter of the mouse renin gene critical for expression
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1107 - F1115.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
L. J. Mullins and J. J. Mullins
Large transgenes reveal their secrets. Focus on "Differential expression of the closely linked KISS1, REN, and FLJ10761 genes in transgenic mice"
Physiol Genomics, March 12, 2004; 17(1): 1 - 3.
[Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
R. Nistala, X. Zhang, and C. D. Sigmund
Differential expression of the closely linked KISS1, REN, and FLJ10761 genes in transgenic mice
Physiol Genomics, March 12, 2004; 17(1): 4 - 10.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. T. Todorov, S. Volkl, M. Muller, A. Bohla, J. Klar, L. A. Kunz-Schughart, T. Hehlgans, and A. Kurtz
Tumor Necrosis Factor-{alpha} Activates NF{kappa}B to Inhibit Renin Transcription by Targeting cAMP-responsive Element
J. Biol. Chem., January 9, 2004; 279(2): 1458 - 1467.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. B. Persson, A. Skalweit, R. Mrowka, and B.-J. Thiele
Control of renin synthesis
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2003; 285(3): R491 - R497.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
X. Liu, X. Huang, and C. D. Sigmund
Identification of a Nuclear Orphan Receptor (Ear2) as a Negative Regulator of Renin Gene Transcription
Circ. Res., May 16, 2003; 92(9): 1033 - 1040.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. Mrowka, K. Steinhage, A. Patzak, and P. B. Persson
An evolutionary approach for identifying potential transcription factor binding sites: the renin gene as an example
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2003; 284(4): R1147 - R1150.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. J. Ryan, T. A. Black, S. L. Millard, K. W. Gross, and G. Hajduczok
Endothelin-1 increases calcium and attenuates renin gene expression in As4.1 cells
Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2458 - H2465.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Nistala and C. D. Sigmund
A reliable and efficient method for deleting operational sequences in PACs and BACs
Nucleic Acids Res., May 15, 2002; 30(10): e41 - e41.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Pan, T. A. Black, Q. Shi, C. A. Jones, N. Petrovic, J. Loudon, C. Kane, C. D. Sigmund, and K. W. Gross
Critical Roles of a Cyclic AMP Responsive Element and an E-box in Regulation of Mouse Renin Gene Expression
J. Biol. Chem., November 30, 2001; 276(49): 45530 - 45538.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
Q. Shi, K. W. Gross, and C. D. Sigmund
NF-Y Antagonizes Renin Enhancer Function by Blocking Stimulatory Transcription Factors
Hypertension, September 1, 2001; 38(3): 332 - 336.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
H. L. Keen and C. D. Sigmund
Paradoxical Regulation of Short Promoter Human Renin Transgene by Angiotensin II
Hypertension, February 1, 2001; 37(2): 403 - 407.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
J. P. Abonia, P. N. Howles, K. J. Abel, T. A. Black, C. A. Jones, and K. W. Gross
Evaluating a Model of an NRE Mediated Tissue-Specific Expression of Murine Renin Genes
Hypertension, January 1, 2001; 37(1): 105 - 109.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
C. A. JONES, M. I. HURLEY, T. A. BLACK, C. M. KANE, L. PAN, S. C. PRUITT, and K. W. GROSS
Expression of a renin/GFP transgene in mouse embryonic, extra-embryonic, and adult tissues
Physiol Genomics, November 9, 2000; 4(1): 75 - 81.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. J. Ryan, K. W. Gross, and G. Hajduczok
Calcium-dependent activation of phospholipase C by mechanical distension in renin-expressing As4.1 cells
Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E823 - E829.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. J. Ryan, T. A. Black, K. W. Gross, and G. Hajduczok
Cyclic mechanical distension regulates renin gene transcription in As4.1 cells
Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E830 - E837.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Q. Jiang, M. Troyanovskaya, G. Jayaraman, and D. P. Healy
Aminopeptidase-A. II. Genomic cloning and characterization of the rat promoter
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2000; 278(2): R425 - R434.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. L. Sinn, D. R. Davis, and C. D. Sigmund
Highly Regulated Cell Type-restricted Expression of Human Renin in Mice Containing 140- or 160-Kilobase Pair P1 Phage Artificial Chromosome Transgenes
J. Biol. Chem., December 10, 1999; 274(50): 35785 - 35793.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. L. Sinn, X. Zhang, and C. D. Sigmund
JG cell expression and partial regulation of a human renin genomic transgene driven by a minimal renin promoter
Am J Physiol Renal Physiol, October 1, 1999; 277(4): F634 - F642.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Q. Shi, T. A. Black, K. W. Gross, and C. D. Sigmund
Species-Specific Differences in Positive and Negative Regulatory Elements in the Renin Gene Enhancer
Circ. Res., September 17, 1999; 85(6): 479 - 488.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Y. Yan, R. Chen, T. Pitarresi, C. D. Sigmund, K. W. Gross, J. E. Sealey, J. H. Laragh, and D. F. Catanzaro
Kidney Is the Only Source of Human Plasma Renin in 45-kb Human Renin Transgenic Mice
Circ. Res., December 14, 1998; 83(12): 1279 - 1288.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Germain, F. Bonnet, J. Philippe, S. Fuchs, P. Corvol, and F. Pinet
A Novel Distal Enhancer Confers Chorionic Expression on the Human Renin Gene
J. Biol. Chem., September 25, 1998; 273(39): 25292 - 25300.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Y. Yan, C. A. Jones, C. D. Sigmund, K. W. Gross, and D. F. Catanzaro
Conserved Enhancer Elements in Human and Mouse Renin Genes Have Different Transcriptional Effects in As4.1 Cells
Circ. Res., October 19, 1997; 81(4): 558 - 566.
[Abstract] [Full Text]

Home page
 HypertensionHome page
N. Petrovic, C. M. Kane, C. D. Sigmund, and K. W. Gross
Downregulation of Renin Gene Expression by Interleukin-1
Hypertension, August 1, 1997; 30(2): 230 - 235.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
K. Tamura, S. Umemura, N. Nyui, S. Yamaguchi, T. Ishigami, K. Hibi, M. Yabana, M. Kihara, A. Fukamizu, K. Murakami, et al.
A Novel Proximal Element Mediates the Regulation of Mouse Ren-1C Promoter by Retinoblastoma Protein in Cultured Cells
J. Biol. Chem., July 4, 1997; 272(27): 16845 - 16851.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Baumann, Y. Wang, C. D. Richards, C. A. Jones, T. A. Black, and K. W. Gross
Endotoxin-induced Renal Inflammatory Response. ONCOSTATIN M AS A MAJOR MEDIATOR OF SUPPRESSED RENIN EXPRESSION
J. Biol. Chem., July 14, 2000; 275(29): 22014 - 22019.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Shi, K. W. Gross, and C. D. Sigmund
Retinoic Acid-mediated Activation of the Mouse Renin Enhancer
J. Biol. Chem., January 26, 2001; 276(5): 3597 - 3603.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Pan, Y. Xie, T. A. Black, C. A. Jones, S. C. Pruitt, and K. W. Gross
An Abd-B Class HOX{middle dot}PBX Recognition Sequence Is Required for Expression from the Mouse Ren-1c Gene
J. Biol. Chem., August 24, 2001; 276(35): 32489 - 32494.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.