|
|
|
|||||||
|
|||||||||
(Received for publication, March 14, 1996, and in revised form, June 17, 1996)
From the Osteopontin is an arginine-glycine-aspartic
acid-containing cell adhesion protein, which is frequently expressed in
transformed cells and is thought to play a role in tumorigenesis. v-Src
is a transforming viral oncogene product encoded by Rous sarcoma virus
(RSV). We report that v-Src expression in HT1080 fibrosarcoma cells
significantly stimulates mouse osteopontin promoter activity. We also
determined the v-Src response element in the osteopontin promoter as an
inverted CCAAT box located at
Volume 271, Number 37,
Issue of September 13, 1996
pp. 22713-22717
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
,
and
Department of Bone Biology and Osteoporosis
Research, Merck Research Laboratories, West Point, Pennsylvania 19486 and § Department of Biological Sciences, Rutgers University,
Piscataway, New Jersey 08855-1059
53 to 49 from the transcription start
site. Mutations of the CCAAT box disrupts protein-DNA interaction and
diminishes both v-Src stimulation and basal promoter activity. A CCAAT
box-containing fragment corresponding to 155 to 122 of RSV long
terminal repeat competed with the 72 to 38 fragment of mouse
osteopontin promoter for specific protein binding in the gel shift
assay. A polyclonal antibody against CBF, a CCAAT box-binding factor,
supershifted in gel shift assays the protein-DNA complex formed by
nuclear extract of HT1080 with either the RSV CCAAT box fragment or
with the osteopontin 72 to 38 fragment. Moreover, both osteopontin
mRNA levels and enhancer activity of CCAAT box-containing 72 to
38 fragment were significantly elevated in
v-src-transformed NIH 3T3 cells relative to parental cells.
These findings suggest that the elevated osteopontin expression in
transformed cells could be due, at least in part, to v-Src stimulation
of the osteopontin promoter and that this effect is mediated by a
CBF-like factor.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. A. Whalen, G. F. Weber, T. L. Benjamin, and B. S. Schaffhausen Polyomavirus Middle T Antigen Induces the Transcription of Osteopontin, a Gene Important for the Migration of Transformed Cells J. Virol., May 15, 2008; 82(10): 4946 - 4954. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. R. Rodrigues, J. A. Teixeira, F. L. Schmitt, M. Paulsson, and H. Lindmark-Mansson The Role of Osteopontin in Tumor Progression and Metastasis in Breast Cancer Cancer Epidemiol. Biomarkers Prev., June 1, 2007; 16(6): 1087 - 1097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Schapira, G. Lazer, and S. Katzav Osteopontin Is an Oncogenic Vav1- but not Wild-type Vav1-Responsive Gene: Implications for Fibroblast Transformation. Cancer Res., June 15, 2006; 66(12): 6183 - 6191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-A. Renault, S. Jalvy, I. Belloc, S. Pasquet, S. Sena, M. Olive, C. Desgranges, and A.-P. Gadeau AP-1 Is Involved in UTP-Induced Osteopontin Expression in Arterial Smooth Muscle Cells Circ. Res., October 3, 2003; 93(7): 674 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Samoto, E. Shimizu, Y. Matsuda-Honjyo, R. Saito, S. Nakao, M. Yamazaki, S. Furuyama, H. Sugiya, J. Sodek, and Y. Ogata Prostaglandin E2 Stimulates Bone Sialoprotein (BSP) Expression through cAMP and Fibroblast Growth Factor 2 Response Elements in the Proximal Promoter of the Rat BSP Gene J. Biol. Chem., August 1, 2003; 278(31): 28659 - 28667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Nam, Y. Ino, M. Sakamoto, and S. Hirohashi Src Family Kinase Inhibitor PP2 Restores the E-Cadherin/Catenin Cell Adhesion System in Human Cancer Cells and Reduces Cancer Metastasis Clin. Cancer Res., July 1, 2002; 8(7): 2430 - 2436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Menke, C. Philippi, R. Vogelmann, B. Seidel, M. P. Lutz, G. Adler, and D. Wedlich Down-Regulation of E-Cadherin Gene Expression by Collagen Type I and Type III in Pancreatic Cancer Cell Lines Cancer Res., April 1, 2001; 61(8): 3508 - 3517. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Marzia, N. A. Sims, S. Voit, S. Migliaccio, A. Taranta, S. Bernardini, T. Faraggiana, T. Yoneda, G. R. Mundy, B. F. Boyce, et al. Decreased c-Src Expression Enhances Osteoblast Differentiation and Bone Formation J. Cell Biol., October 18, 2000; 151(2): 311 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nemir, D. Bhattacharyya, X. Li, K. Singh, A. B. Mukherjee, and B. B. Mukherjee Targeted Inhibition of Osteopontin Expression in the Mammary Gland Causes Abnormal Morphogenesis and Lactation Deficiency J. Biol. Chem., January 14, 2000; 275(2): 969 - 976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sodek, B. Ganss, and M.D. McKee Osteopontin Critical Reviews in Oral Biology & Medicine, January 1, 2000; 11(3): 279 - 303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kusnetsov, M. Landsberger, J. Meurer, and R. Oelmuller The Assembly of the CAAT-box Binding Complex at a Photosynthesis Gene Promoter Is Regulated by Light, Cytokinin, and the Stage of the Plastids J. Biol. Chem., December 10, 1999; 274(50): 36009 - 36014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Kim and J. Sodek Transcription of the Bone Sialoprotein Gene Is Stimulated by v-Src Acting through an Inverted CCAAT Box Cancer Res., February 1, 1999; 59(3): 565 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Bi, L. Wu, F. Coustry, B. de Crombrugghe, and S. N. Maity DNA Binding Specificity of the CCAAT-binding Factor CBF/NF-Y J. Biol. Chem., October 17, 1997; 272(42): 26562 - 26572. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |