| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, April 23, 1996, and in revised form, June 21, 1996)
From the We have isolated and characterized cDNA
clones encoding the human and rat Na,K-ATPase
Volume 271, Number 37,
Issue of September 13, 1996
pp. 22754-22758
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
3 Subunit
,
,
Department of Pharmacology, Penn State
College of Medicine, Milton S. Hershey Medical Center, Hershey,
Pennsylvania 17033 and the § Department of Biochemistry,
McGill University, Montreal, Quebec, Canada H3G 1Y6
3 subunit isoform. The
human cDNA encodes a polypeptide of 279 amino acids that exhibits
primary sequence and secondary structure similarities to Na,K-ATPase
subunit isoforms. Sequence comparisons showed that the human 3
subunit closely resembles the 3 subunit of Xenopus
laevis (59% amino acid identity) and is less similar to the
human Na,K-ATPase 1 and 2 subunits (38% and 48% amino acid
identity, respectively). By analyzing the segregation of restriction
fragment length polymorphisms among recombinant inbred strains of mice,
we localized the 3 subunit gene to murine chromosome 7. Northern
blot analysis revealed that the 3 subunit gene encodes two
transcripts that are expressed in a variety of rat tissues including
testis, brain, kidney, lung, stomach, small intestine, colon, spleen,
and liver. Identification of the mammalian 3 subunit suggests an
even greater potential for Na,K-ATPase isoenzyme diversity than
previously realized.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Phakdeekitcharoen, S. Phudhichareonrat, C. Pookarnjanamorakot, C. Kijkunasathian, N. Tubtong, W. Kittikanokrat, and P. Radinahamed Thyroid Hormone Increases mRNA and Protein Expression of Na+-K+-ATPase {alpha}2 and {beta}1 Subunits in Human Skeletal Muscles J. Clin. Endocrinol. Metab., January 1, 2007; 92(1): 353 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Sanchez, A.-N. T. Nguyen, B. Timmerberg, J. S. Tash, and G. Blanco The Na,K-ATPase {alpha}4 isoform from humans has distinct enzymatic properties and is important for sperm motility Mol. Hum. Reprod., September 1, 2006; 12(9): 565 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cereijido, R. G. Contreras, and L. Shoshani Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans Physiol Rev, October 1, 2004; 84(4): 1229 - 1262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T. Murphy, R. J. Snow, A. C. Petersen, R. M. Murphy, J. Mollica, J. S. Lee, A. P. Garnham, R. J. Aughey, J. A. Leppik, I. Medved, et al. Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle J. Physiol., April 15, 2004; 556(2): 507 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shao and F. Ismail-Beigi Control of Na+-K+-ATPase {beta}1-subunit expression: role of 3'-untranslated region Am J Physiol Cell Physiol, March 1, 2004; 286(3): C580 - C585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Ng, M. Nagarajan, K. N. Jew, L. C. Mace, and R. L. Moore Exercise training differentially modifies age-associated alteration in expression of Na+-K+-ATPase subunit isoforms in rat skeletal muscles Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2003; 285(4): R733 - R740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Huang, G. Blanco, R. W. Mercer, T. Fleming, and J. S. Pepose Human Corneal Endothelial Cell Expression of Na+,K+-Adenosine Triphosphatase Isoforms Arch Ophthalmol, June 1, 2003; 121(6): 840 - 845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chiampanichayakul, A. Szekeres, P. Khunkaewla, S. Moonsom, V. Leksa, K. Drbal, G. J. Zlabinger, R. Hofer-Warbinek, H. Stockinger, and W. Kasinrerk Engagement of Na,K-ATPase {beta}3 subunit by a specific mAb suppresses T and B lymphocyte activation Int. Immunol., December 1, 2002; 14(12): 1407 - 1414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. R. Rajarao, V. A. Canfield, M.-A. P.K. Mohideen, Y.-L. Yan, J. H. Postlethwait, K. C. Cheng, and R. Levenson The Repertoire of Na,K-ATPase alpha and beta Subunit Genes Expressed in the Zebrafish, Danio rerio Genome Res., July 1, 2001; 11(7): 1211 - 1220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Wetzel and K. J. Sweadner Immunocytochemical Localization of NaK-ATPase Isoforms in the Rat and Mouse Ocular Ciliary Epithelium Invest. Ophthalmol. Vis. Sci., March 1, 2001; 42(3): 763 - 769. [Abstract] [Full Text]
|
|
|
|
|
|
C. B. Thompson, I. Dorup, J. Ahn, P. K. K. Leong, and A. A. McDonough Glucocorticoids increase sodium pump {alpha}2- and {beta}1-subunit abundance and mRNA in rat skeletal muscle Am J Physiol Cell Physiol, March 1, 2001; 280(3): C509 - C516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Michea, V. Irribarra, I. A. Goecke, and E. T. Marusic Reduced Na-K pump but increased Na-K-2Cl cotransporter in aorta of streptozotocin-induced diabetic rat Am J Physiol Heart Circ Physiol, February 1, 2001; 280(2): H851 - H858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Blanco, G. Sánchez, R. J. Melton, W. G. Tourtellotte, and R. W. Mercer The {alpha}4 Isoform of the Na,K-ATPase Is Expressed in the Germ Cells of the Testes J. Histochem. Cytochem., August 1, 2000; 48(8): 1023 - 1032. [Abstract] [Full Text]
|
|
|
|
|
|
C. T. Okamoto, D. C. Chow, and A. J. G. Forte Interaction of alpha - and beta -subunits in native H-K-ATPase and cultured cells transfected with H-K-ATPase beta -subunit Am J Physiol Cell Physiol, April 1, 2000; 278(4): C727 - C738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sangan, S. Thevananther, S. Sangan, V. M. Rajendran, and H. J. Binder Colonic H-K-ATPase alpha - and beta -subunits express ouabain-insensitive H-K-ATPase Am J Physiol Cell Physiol, January 1, 2000; 278(1): C182 - C189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Sun, M. Nagarajan, P. W. Beesley, and Y.-C. Ng Age-associated differential expression of Na+-K+-ATPase subunit isoforms in skeletal muscles of F-344/BN rats J Appl Physiol, September 1, 1999; 87(3): 1132 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Thompson, C. Choi, J. H. Youn, and A. A. McDonough Temporal responses of oxidative vs. glycolytic skeletal muscles to K+ deprivation: Na+ pumps and cell cations Am J Physiol Cell Physiol, June 1, 1999; 276(6): C1411 - C1419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Jaisser and A. T. Beggah The nongastric H+-K+-ATPases: molecular and functional properties Am J Physiol Renal Physiol, June 1, 1999; 276(6): F812 - F824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sangan, S. S. Kolla, V. M. Rajendran, M. Kashgarian, and H. J. Binder Colonic H-K-ATPase beta -subunit: identification in apical membranes and regulation by dietary K depletion Am J Physiol Cell Physiol, February 1, 1999; 276(2): C350 - C360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Michea, V. Valenzuela, I. Bravo, A. Schuster, and E. T. Marusic Adrenal-dependent modulation of the catalytic subunit isoforms of the Na+-K+-ATPase in aorta Am J Physiol Endocrinol Metab, December 1, 1998; 275(6): E1072 - E1081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Weber, U. Bartsch, M. Schachner, and D. Montag Na,K-ATPase Subunit beta 1 knock-in Prevents Lethality of beta 2 Deficiency in Mice J. Neurosci., November 15, 1998; 18(22): 9192 - 9203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Blanco and R. W. Mercer Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function Am J Physiol Renal Physiol, November 1, 1998; 275(5): F633 - F650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Borok, S. I. Danto, L. L. Dimen, X.-L. Zhang, and R. L. Lubman Na+-K+-ATPase expression in alveolar epithelial cells: upregulation of active ion transport by KGF Am J Physiol Lung Cell Mol Physiol, January 1, 1998; 274(1): L149 - L158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Jones, T. C. Davies, and G. M. Kidder Embryonic Expression of the Putative gamma Subunit of the Sodium Pump Is Required for Acquisition of Fluid Transport Capacity during Mouse Blastocyst Development J. Cell Biol., December 15, 1997; 139(6): 1545 - 1552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Arystarkhova and K. J. Sweadner Tissue-specific Expression of the Na,K-ATPase beta 3 Subunit. THE PRESENCE OF beta 3 IN LUNG AND LIVER ADDRESSES THE PROBLEM OF THE MISSING SUBUNIT J. Biol. Chem., September 5, 1997; 272(36): 22405 - 22408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Stengelin and J. F. Hoffman Na,K-ATPase subunit isoforms in human reticulocytes: Evidence from reverse transcription-PCR for the presence of alpha 1, alpha 3, beta 2, beta 3, and gamma PNAS, May 27, 1997; 94(11): 5943 - 5948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Peng, P. Martin-Vasallo, and K. J. Sweadner Isoforms of Na,K-ATPase alpha and beta Subunits in the Rat Cerebellum and in Granule Cell Cultures J. Neurosci., May 15, 1997; 17(10): 3488 - 3502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Woo, P. F. James, and J. B Lingrel Sperm Motility Is Dependent on a Unique Isoform of the Na,K-ATPase J. Biol. Chem., June 30, 2000; 275(27): 20693 - 20699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhuang, C. Wendt, and G. Gick Regulation of Na,K-ATPase beta 1 Subunit Gene Transcription by Low External Potassium in Cardiac Myocytes. ROLE OF Sp1 AND Sp3 J. Biol. Chem., July 28, 2000; 275(31): 24173 - 24184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. Serluca, A. Sidow, J. D. Mably, and M. C. Fishman Partitioning of Tissue Expression Accompanies Multiple Duplications of the Na+/K+ ATPase alpha Subunit Gene Genome Res., October 1, 2001; 11(10): 1625 - 1631. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
