HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thompson, A. Articles by Ravid, K. Search for Related Content PubMed PubMed Citation Articles by Thompson, A. Articles by Ravid, K. Social Bookmarking                      What's this?

Volume 271, Number 38, Issue of September 20, 1996 pp. 22976-22982
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Deregulated Expression of c-myc in Megakaryocytes of Transgenic Mice Increases Megakaryopoiesis and Decreases Polyploidization

(Received for publication, January 25, 1996, and in revised form, May 31, 1996)

Alexander Thompson , Ying Zhang , Dimitry Kamen , Carl W. Jackson ^§ , Robert D. Cardiff ^¶ and Katya Ravid

From the  Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02139, the ^§ Division of Experimental Hematology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, and the ^¶ Department of Medical Pathology, University of California at Davis, Sacramento, California 95817

Platelets, essential for vascular integrity and hemostasis, fragment from polyploid megakaryocytes, characterized by their endomitotic cell cycle. We studied the influence of overexpression of c-myc oncogene on megakaryopoiesis and endomitosis in vivo, using transgenic mice carrying c-myc fused to the estrogen receptor under the control of the platelet factor 4 (PF4) megakaryocyte-specific promoter. The rationale behind this strategy was to obtain controlled overexpression of an active c-Myc, depending on the estrogen level in the mouse circulation. Analysis of these transgenic mice revealed that the bone marrow of female transgenic mice or of estrogen-injected male transgenic mice, but not of age-matched transgenic males nor nontransgenic females, contained frequent immature myeloid cells and an increased number of megakaryocytes. Deregulated expression of c-Myc shifted the normal ploidy profile of megakaryocytes due to a significant increase in proliferating megakaryocytes and a decrease in the fraction of ploidizing cells. These transgenic mice represent a novel in vivo model for a Myc-induced myeloproliferative disorder which can be controlled.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. G. Fuhrken, C. Chen, P. A. Apostolidis, M. Wang, W. M. Miller, and E. T. Papoutsakis Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesis Physiol Genomics, April 1, 2008; 33(2): 159 - 169. [Abstract] [Full Text] [PDF]

				H. G. Nguyen, G. Yu, M. Makitalo, D. Yang, H.-X. Xie, M. R. Jones, and K. Ravid Conditional overexpression of transgenes in megakaryocytes and platelets in vivo Blood, September 1, 2005; 106(5): 1559 - 1564. [Abstract] [Full Text] [PDF]

				W. Tong and H. F. Lodish Lnk Inhibits Tpo-mpl Signaling and Tpo-mediated Megakaryocytopoiesis J. Exp. Med., September 7, 2004; 200(5): 569 - 580. [Abstract] [Full Text] [PDF]

				Y. Zhang, Y. Nagata, G. Yu, H. G. Nguyen, M. R. Jones, P. Toselli, C. W. Jackson, M. Tatsuka, K. Todokoro, and K. Ravid Aberrant quantity and localization of Aurora-B/AIM-1 and survivin during megakaryocyte polyploidization and the consequences of Aurora-B/AIM-1-deregulated expression Blood, May 15, 2004; 103(10): 3717 - 3726. [Abstract] [Full Text] [PDF]

				J. G. Drachman, Y. Miyakawa, J. N. Luthi, D. D. Dahlen, A. Raney, A. E. Geddis, and K. Kaushansky Studies with Chimeric Mpl/JAK2 Receptors Indicate That Both JAK2 and the Membrane-proximal Domain of Mpl Are Required for Cellular Proliferation J. Biol. Chem., June 21, 2002; 277(26): 23544 - 23553. [Abstract] [Full Text] [PDF]

				K. Kirito, M. Osawa, H. Morita, R. Shimizu, M. Yamamoto, A. Oda, H. Fujita, M. Tanaka, K. Nakajima, Y. Miura, et al. A functional role of Stat3 in in vivo megakaryopoiesis Blood, May 1, 2002; 99(9): 3220 - 3227. [Abstract] [Full Text] [PDF]

				B. D. Car and V. M. Eng Special Considerations in the Evaluation of the Hematology and Hemostasis of Mutant Mice Vet. Pathol., January 1, 2001; 38(1): 20 - 30. [Abstract] [Full Text] [PDF]

				Z. Shu, S. Smith, L. Wang, M. C. Rice, and E. B. Kmiec Disruption of muREC2/RAD51L1 in Mice Results in Early Embryonic Lethality Which Can Be Partially Rescued in a p53-/- Background Mol. Cell. Biol., December 1, 1999; 19(12): 8686 - 8693. [Abstract] [Full Text] [PDF]

				Z. Cui, M. P. Reilly, S. Surrey, E. Schwartz, and S. E. McKenzie -245 bp of 5'-Flanking Region From the Human Platelet Factor 4 Gene Is Sufficient to Drive Megakaryocyte-Specific Expression In Vivo Blood, April 1, 1998; 91(7): 2326 - 2333. [Abstract] [Full Text] [PDF]