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(Received for publication, November 13, 1996, and in revised form, March 25, 1996)
From the Centro de Biología Molecular, CSIC-UAM,
Universidad Autónoma de Madrid, Canto Blanco,
28049 Madrid, Spain
Poliovirus infection leads to drastic alterations
in membrane permeability late during infection. Transient expression of
each nonstructural protein of poliovirus by means of recombinant
vaccinia virus encoding the T7 RNA polymerase indicates that proteins
2B and 2BC strongly enhance membrane permeability to hygromycin B in
HeLa cells. Almost no effect on expression of proteins 2C, 3A, 3AB, and
3C was found. Deletions and point mutations in 2B and 2BC have
identified sequences in 2B involved in membrane permeabilization.
Regions located at both ends of 2B are necessary to bring about these
permeability alterations. A deletion of 11 amino acids of 2BC at the
junction between 2B and 2C, as well as long deletions in 2C
encompassing the GTPase motifs of this protein, do not impair the
capacity of 2BC to modify the permeability of the membrane. The release
of compounds such as choline or uridine from preloaded cells is also
augmented by 2B and 2BC expression.
Volume 271, Number 38,
Issue of September 20, 1996
pp. 23134-23137
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
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