|
|
|
|||||||
|
|||||||||
(Received for publication, November 13, 1996, and in revised form, March 25, 1996)
From the Centro de Biología Molecular, CSIC-UAM,
Universidad Autónoma de Madrid, Canto Blanco,
28049 Madrid, Spain
Poliovirus infection leads to drastic alterations
in membrane permeability late during infection. Transient expression of
each nonstructural protein of poliovirus by means of recombinant
vaccinia virus encoding the T7 RNA polymerase indicates that proteins
2B and 2BC strongly enhance membrane permeability to hygromycin B in
HeLa cells. Almost no effect on expression of proteins 2C, 3A, 3AB, and
3C was found. Deletions and point mutations in 2B and 2BC have
identified sequences in 2B involved in membrane permeabilization.
Regions located at both ends of 2B are necessary to bring about these
permeability alterations. A deletion of 11 amino acids of 2BC at the
junction between 2B and 2C, as well as long deletions in 2C
encompassing the GTPase motifs of this protein, do not impair the
capacity of 2BC to modify the permeability of the membrane. The release
of compounds such as choline or uridine from preloaded cells is also
augmented by 2B and 2BC expression.
Volume 271, Number 38,
Issue of September 20, 1996
pp. 23134-23137
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. M. De Palma, H. J. Thibaut, L. van der Linden, K. Lanke, W. Heggermont, S. Ireland, R. Andrews, M. Arimilli, T. H. Al-Tel, E. De Clercq, et al. Mutations in the Nonstructural Protein 3A Confer Resistance to the Novel Enterovirus Replication Inhibitor TTP-8307 Antimicrob. Agents Chemother., May 1, 2009; 53(5): 1850 - 1857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Armer, K. Moffat, T. Wileman, G. J. Belsham, T. Jackson, W. P. Duprex, M. Ryan, and P. Monaghan Foot-and-Mouth Disease Virus, but Not Bovine Enterovirus, Targets the Host Cell Cytoskeleton via the Nonstructural Protein 3Cpro J. Virol., November 1, 2008; 82(21): 10556 - 10566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yin, Y. Liu, E. Wimmer, and A. V. Paul Complete protein linkage map between the P2 and P3 non-structural proteins of poliovirus J. Gen. Virol., August 1, 2007; 88(8): 2259 - 2267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Cornell, W. B. Kiosses, S. Harkins, and J. L. Whitton Coxsackievirus B3 Proteins Directionally Complement Each Other To Downregulate Surface Major Histocompatibility Complex Class I J. Virol., July 1, 2007; 81(13): 6785 - 6797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Krogerus, O. Samuilova, T. Poyry, E. Jokitalo, and T. Hyypia Intracellular localization and effects of individually expressed human parechovirus 1 non-structural proteins J. Gen. Virol., March 1, 2007; 88(3): 831 - 841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Stuart and T. D. K. Brown Entry of feline calicivirus is dependent on clathrin-mediated endocytosis and acidification in endosomes. J. Virol., August 1, 2006; 80(15): 7500 - 7509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. de Jong, H.-J. Visch, F. de Mattia, M. M. van Dommelen, H. G. Swarts, T. Luyten, G. Callewaert, W. J. Melchers, P. H. Willems, and F. J. van Kuppeveld The Coxsackievirus 2B Protein Increases Efflux of Ions from the Endoplasmic Reticulum and Golgi, thereby Inhibiting Protein Trafficking through the Golgi J. Biol. Chem., May 19, 2006; 281(20): 14144 - 14150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Zhang, G. Zhang, L. Yang, J. Chisholm, and H. Sanfacon Evidence that Insertion of Tomato Ringspot Nepovirus NTB-VPg Protein in Endoplasmic Reticulum Membranes Is Directed by Two Domains: a C-Terminal Transmembrane Helix and an N-Terminal Amphipathic Helix J. Virol., September 15, 2005; 79(18): 11752 - 11765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Salsman, D. Top, J. Boutilier, and R. Duncan Extensive Syncytium Formation Mediated by the Reovirus FAST Proteins Triggers Apoptosis-Induced Membrane Instability J. Virol., July 1, 2005; 79(13): 8090 - 8100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Harris and V. R. Racaniello Amino Acid Changes in Proteins 2B and 3A Mediate Rhinovirus Type 39 Growth in Mouse Cells J. Virol., May 1, 2005; 79(9): 5363 - 5373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Moffat, G. Howell, C. Knox, G. J. Belsham, P. Monaghan, M. D. Ryan, and T. Wileman Effects of Foot-and-Mouth Disease Virus Nonstructural Proteins on the Structure and Function of the Early Secretory Pathway: 2BC but Not 3A Blocks Endoplasmic Reticulum-to-Golgi Transport J. Virol., April 1, 2005; 79(7): 4382 - 4395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yanagiya, Q. Jia, S. Ohka, H. Horie, and A. Nomoto Blockade of the Poliovirus-Induced Cytopathic Effect in Neural Cells by Monoclonal Antibody against Poliovirus or the Human Poliovirus Receptor J. Virol., February 1, 2005; 79(3): 1523 - 1532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Han and R. N. Harty The NS3 Protein of Bluetongue Virus Exhibits Viroporin-like Properties J. Biol. Chem., October 8, 2004; 279(41): 43092 - 43097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Choe and K. Kirkegaard Intracellular Topology and Epitope Shielding of Poliovirus 3A Protein J. Virol., June 1, 2004; 78(11): 5973 - 5982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. de Jong, W. J. G. Melchers, D. H. R. F. Glaudemans, P. H. G. M. Willems, and F. J. M. van Kuppeveld Mutational Analysis of Different Regions in the Coxsackievirus 2B Protein: REQUIREMENTS FOR HOMO-MULTIMERIZATION, MEMBRANE PERMEABILIZATION, SUBCELLULAR LOCALIZATION, AND VIRUS REPLICATION J. Biol. Chem., May 7, 2004; 279(19): 19924 - 19935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Campanella, A. S. de Jong, K. W. H. Lanke, W. J. G. Melchers, P. H. G. M. Willems, P. Pinton, R. Rizzuto, and F. J. M. van Kuppeveld The Coxsackievirus 2B Protein Suppresses Apoptotic Host Cell Responses by Manipulating Intracellular Ca2+ Homeostasis J. Biol. Chem., April 30, 2004; 279(18): 18440 - 18450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Harris and V. R. Racaniello Changes in Rhinovirus Protein 2C Allow Efficient Replication in Mouse Cells J. Virol., April 15, 2003; 77(8): 4773 - 4780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. de Jong, E. Wessels, H. B. P. M. Dijkman, J. M. D. Galama, W. J. G. Melchers, P. H. G. M. Willems, and F. J. M. van Kuppeveld Determinants for Membrane Association and Permeabilization of the Coxsackievirus 2B Protein and the Identification of the Golgi Complex as the Target Organelle J. Biol. Chem., January 3, 2003; 278(2): 1012 - 1021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Blanco, L. Carrasco, and I. Ventoso Cell Killing by HIV-1 Protease J. Biol. Chem., January 3, 2003; 278(2): 1086 - 1093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jurgens and J. B. Flanegan Initiation of Poliovirus Negative-Strand RNA Synthesis Requires Precursor Forms of P2 Proteins J. Virol., December 20, 2002; 77(2): 1075 - 1083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Agirre, A. Barco, L. Carrasco, and J. L. Nieva Viroporin-mediated Membrane Permeabilization. PORE FORMATION BY NONSTRUCTURAL POLIOVIRUS 2B PROTEIN J. Biol. Chem., October 18, 2002; 277(43): 40434 - 40441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. M. van Kuppeveld, W. J. G. Melchers, P. H. G. M. Willems, and T. W. J. Gadella Jr. Homomultimerization of the Coxsackievirus 2B Protein in Living Cells Visualized by Fluorescence Resonance Energy Transfer Microscopy J. Virol., August 12, 2002; 76(18): 9446 - 9456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Sosnovtsev, M. Garfield, and K. Y. Green Processing Map and Essential Cleavage Sites of the Nonstructural Polyprotein Encoded by ORF1 of the Feline Calicivirus Genome J. Virol., June 14, 2002; 76(14): 7060 - 7072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Carette, J. van Lent, S. A. MacFarlane, J. Wellink, and A. van Kammen Cowpea Mosaic Virus 32- and 60-Kilodalton Replication Proteins Target and Change the Morphology of Endoplasmic Reticulum Membranes J. Virol., May 13, 2002; 76(12): 6293 - 6301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bodelon, L. Labrada, J. Martinez-Costas, and J. Benavente Modification of Late Membrane Permeability in Avian Reovirus-infected Cells. VIROPORIN ACTIVITY OF THE S1-ENCODED NONSTRUCTURAL p10 PROTEIN J. Biol. Chem., May 10, 2002; 277(20): 17789 - 17796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. de Jong, I. W. J. Schrama, P. H. G. M. Willems, J. M. D. Galama, W. J. G. Melchers, and F. J. M. van Kuppeveld Multimerization reactions of coxsackievirus proteins 2B, 2C and 2BC: a mammalian two-hybrid analysis J. Gen. Virol., April 1, 2002; 83(4): 783 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. M. van Kuppeveld, P. J. J. C. van den Hurk, I. W. J. Schrama, J. M. D. Galama, and W. J. G. Melchers Trans-complementation of a genetic defect in the coxsackie B3 virus 2B protein J. Gen. Virol., February 1, 2002; 83(2): 341 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Ciccaglione, A. Costantino, C. Marcantonio, M. Equestre, A. Geraci, and M. Rapicetta Mutagenesis of hepatitis C virus E1 protein affects its membrane-permeabilizing activity J. Gen. Virol., September 1, 2001; 82(9): 2243 - 2250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Egger, N. Teterina, E. Ehrenfeld, and K. Bienz Formation of the Poliovirus Replication Complex Requires Coupled Viral Translation, Vesicle Production, and Viral RNA Synthesis J. Virol., July 15, 2000; 74(14): 6570 - 6580. [Abstract] [Full Text]
|
|
|
|
|
|
H. Shimizu, M. Agoh, Y. Agoh, H. Yoshida, K. Yoshii, T. Yoneyama, A. Hagiwara, and T. Miyamura Mutations in the 2C Region of Poliovirus Responsible for Altered Sensitivity to Benzimidazole Derivatives J. Virol., May 1, 2000; 74(9): 4146 - 4154. [Abstract] [Full Text]
|
|
|
|
|
|
T. Pfister, K. W. Jones, and E. Wimmer A Cysteine-Rich Motif in Poliovirus Protein 2CATPase Is Involved in RNA Replication and Binds Zinc In Vitro J. Virol., January 1, 2000; 74(1): 334 - 343. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-S. Chang, C.-L. Liao, C.-H. Tsao, M.-C. Chen, C.-I Liu, L.-K. Chen, and Y.-L. Lin Membrane Permeabilization by Small Hydrophobic Nonstructural Proteins of Japanese Encephalitis Virus J. Virol., August 1, 1999; 73(8): 6257 - 6264. [Abstract] [Full Text]
|
|
|
|
|
|
T. Pfister and E. Wimmer Characterization of the Nucleoside Triphosphatase Activity of Poliovirus Protein 2C Reveals a Mechanism by Which Guanidine Inhibits Poliovirus Replication J. Biol. Chem., March 12, 1999; 274(11): 6992 - 7001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bolten, D. Egger, R. Gosert, G. Schaub, L. Landmann, and K. Bienz Intracellular Localization of Poliovirus Plus- and Minus-Strand RNA Visualized by Strand-Specific Fluorescent In Situ Hybridization J. Virol., November 1, 1998; 72(11): 8578 - 8585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Barco and L. Carrasco Identification of Regions of Poliovirus 2BC Protein That Are Involved in Cytotoxicity J. Virol., May 1, 1998; 72(5): 3560 - 3570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cuconati, W. Xiang, F. Lahser, T. Pfister, and E. Wimmer A Protein Linkage Map of the P2 Nonstructural Proteins of Poliovirus J. Virol., February 1, 1998; 72(2): 1297 - 1307. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |