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(Received for publication, February 6, 1996, and in revised form, July 23, 1996)
From the Department of Microbiology, Cornell University, Ithaca,
New York 14853
During denitrification, freely diffusible nitric
oxide (NO) is generated for use as a terminal electron acceptor. NO is
produced by nitrite reductase (Nir) and reduced to nitrous oxide by
nitric oxide reductase (Nor). Using Nir and Nor-deficient mutants of
Rhodobacter sphaeroides 2.4.3, we have shown that the
endogenous production of NO or the addition of exogenous NO induces
transcription of certain genes encoding Nir and Nor. A Nor-deficient
strain was found to be capable of expressing wild type levels of
nirK-lacZ and norB-lacZ fusions in medium
unamended with nitrogen oxides. When this experiment is performed in
the presence of hemoglobin, fusion expression is eliminated. NO and the
NO-generator, sodium nitroprusside, can induce expression of both
fusions in a strain lacking Nir and the consequent ability to produce
NO. Sodium nitroprusside cannot induce expression of
nirK-lacZ in a strain lacking the transcriptional activator
NnrR (nitrite and nitric oxide reductase regulator). Addition of the
cyclic nucleotides cAMP and 8-bromoguanosine-cGMP does not result in
expression of either fusion. These results demonstrate that
denitrifying bacteria produce NO as a signal molecule to activate
expression of the genes encoding proteins required for NO
metabolism.
Volume 271, Number 40,
Issue of October 4, 1996
pp. 24382-24388
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
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