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Volume 271, Number 40, Issue of October 4, 1996 pp. 24442-24448
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Synchronized Heat Flux Oscillations in Yeast Cell Populations

(Received for publication, October 11, 1995, and in revised form, May 13, 1996)

Bas Teusink , Christer Larsson ^§ , Jasper Diderich , Peter Richard , Karel van Dam , Lena Gustafsson ^§ and Hans V. Westerhoff

From the  E. C. Slater Institute, BioCentrum, University of Amsterdam, Plantage Muidergracht 12, NL-1018 TV Amsterdam, The Netherlands, the ^§ Department of General and Marin Microbiology, Göteborg University, Medicinaregatan 9C, S-41319 Göteborg, Sweden, and the  Department of Microbial Physiology, Faculty of Biology, Vrije Universiteit, de Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands

Microcalorimetry was adapted to the study of glycolytic oscillations in suspensions of intact yeast cells. A correction procedure was developed for the distortion of the amplitude and phase of the heat signal, caused by the slow response of the calorimeter. This made it possible to observe oscillations in the heat production rate with a period of less than 1 min, and a relative amplitude of 5-10%. By simultaneously measuring the heat flux and concentrations of glycolytic metabolites, and by comparing acetaldehyde-induced phase shifts of the heat flux oscillations with those of NADH oscillations, the heat flux was found to be 100° out of phase with glucose 6-phosphate, 80° out of phase with fructose 1,6-bisphosphate, and in phase with NADH. The flux measurement made possible by microcalorimetry allowed the recognition of (i) changes in metabolic capacity that may affect glycolytic dynamics, (ii) implications of glucose carrier kinetics for glycolytic dynamics and (iii) the continued requirement for an acetaldehyde trapping agent for the oscillations.

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