Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, Y. H.
Right arrow Articles by Rho, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, Y. H.
Right arrow Articles by Rho, H. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 271, Number 40, Issue of October 4, 1996 pp. 24539-24543
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

Transcriptional Activation of the Cu,Zn-Superoxide Dismutase Gene through the AP2 Site by Ginsenoside Rb2 Extracted from a Medicinal Plant, Panax ginseng

(Received for publication, April 2, 1996, and in revised form, July 11, 1996)

Young Ho Kim , Ki Hyun Park Dagger and Hyune Mo Rho

From the Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, Seoul 151-742, Korea and the Dagger  Korea Ginseng and Tobacco Research Institute, Taejon 305-345, Korea

We report here that the ginseng saponins induce the transcription of Cu,Zn-superoxide dismutase gene (SOD1), which is one of the major antioxidant enzymes. Total saponins and panaxatriol did not elevate the level of SOD1, but panaxadiol significantly increased SOD1. Among the panaxadiol fractions, ginsenoside Rb2 was a more specific and more remarkable inducer of the SOD1 gene than ginsenoside Rb1. Deletion analyses of the SOD1 promoter revealed that the proximal promoter is responsible for this induction. Mobility shift assays with cis-elements in the proximal promoter region showed that specific binding of the AP2 transcription factor was significantly increased by treatment with ginsenoside Rb2. Mutations of the AP2 binding sites in the heterologous promoter and natural context systems abolished the transcriptional activation by ginsenoside Rb2. These results suggest that the SOD1 gene was greatly activated by ginsenoside Rb2 through transcription factor AP2 binding sites and its induction.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J Clin PharmacolHome page
L. S. Lee, S. D. Wise, C. Chan, T. L. Parsons, C. Flexner, and P. S. Lietman
Possible Differential Induction of Phase 2 Enzyme and Antioxidant Pathways by American Ginseng, Panax quinquefolius
J. Clin. Pharmacol., May 1, 2008; 48(5): 599 - 609.
[Abstract] [Full Text] [PDF]

Home page
 Evid Based Complement Alternat MedHome page
L. Wang, G. Muxin, H. Nishida, C. Shirakawa, S. Sato, and T. Konishi
Psychological Stress-Induced Oxidative Stress as a Model of Sub-Healthy Condition and the Effect of TCM
Evid. Based Complement. Altern. Med., June 1, 2007; 4(2): 195 - 202.
[Abstract] [Full Text] [PDF]

Home page
 Evid Based Complement Alternat MedHome page
Y. Han, S.-J. Son, M. Akhalaia, A. Platonov, H.-J. Son, K.-H. Lee, Y.-S. Yun, and J.-Y. Song
Modulation of Radiation-Induced Disturbances of Antioxidant Defense Systems by Ginsan
Evid. Based Complement. Altern. Med., December 1, 2005; 2(4): 529 - 536.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Hwang, D. J. Kleinhenz, B. Lassegue, K. K. Griendling, S. Dikalov, and C. M. Hart
Peroxisome proliferator-activated receptor-{gamma} ligands regulate endothelial membrane superoxide production
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C899 - C905.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
T. Shimazu, I. Inoue, N. Araki, Y. Asano, M. Sawada, D. Furuya, H. Nagoya, and J. H. Greenberg
A Peroxisome Proliferator-Activated Receptor-{gamma} Agonist Reduces Infarct Size in Transient but Not in Permanent Ischemia
Stroke, February 1, 2005; 36(2): 353 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. W. E. Rush, M. H. Laughlin, C. R. Woodman, and E. M. Price
SOD-1 expression in pig coronary arterioles is increased by exercise training
Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2068 - H2076.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Y. Yoo, M. S. Chang, and H. M. Rho
The Activation of the Rat Copper/Zinc Superoxide Dismutase Gene by Hydrogen Peroxide through the Hydrogen Peroxide-responsive Element and by Paraquat and Heat Shock through the Same Heat Shock Element
J. Biol. Chem., August 20, 1999; 274(34): 23887 - 23892.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Minc, P. de Coppet, P. Masson, L. Thiery, S. Dutertre, M. Amor-Gueret, and C. Jaulin
The Human Copper-Zinc Superoxide Dismutase Gene (SOD1) Proximal Promoter Is Regulated by Sp1, Egr-1, and WT1 via Non-canonical Binding Sites
J. Biol. Chem., January 1, 1999; 274(1): 503 - 509.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement