|
|
|
|||||||
|
|||||||||
(Received for publication, February 22, 1996, and in revised form, July 19, 1996)
From the Activation of the rat prolactin (rPRL) promoter
by Ras is a prototypical example of tissue-specific transcriptional
regulation in a highly differentiated cell type. Using a series of
site-specific mutations and deletions of the proximal rPRL promoter we
have mapped the major Ras/Raf response element (RRE) to a composite
Ets-1/GHF-1 binding site located between positions
Volume 271, Number 40,
Issue of October 4, 1996
pp. 24639-24648
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
,
,
,
Departments of Medicine and of Biochemistry,
Biophysics and Genetics, Program in Molecular Biology, and Colorado
Cancer Center, University of Colorado Health Sciences Center, Denver,
Colorado 80262 and the ¶ Department of Molecular Genetics, Ohio
State University, Columbus, Ohio 43210
217 and 190.
Mutation of either the Ets-1 or GHF-1 binding sites inhibits Ras and
Raf activation of the rPRL promoter, and insertion of this RRE into the
rat growth hormone promoter confers Ras responsiveness. We show that
Ets-1 is expressed in GH4 cells and, consistent with their
functional synergistic interaction, both Ets-1 and GHF-1 are able to
bind specifically to this bipartite RRE. We confirm that Ets-1 or a
related Ets factor is the nuclear target of the Ras pathway leading to
activation of the rPRL promoter and demonstrate that Elk-1 and Net do
not mediate the Ras response. Thus, the pituitary-specific POU
homeodomain transcription factor, GHF-1, serves as a cell-specific
signal integrator by functionally interacting with an Ets-1-like
factor, at uniquely juxtaposed binding sites, thereby targeting an
otherwise ubiquitous Ras signaling pathway to a select subset of
cell-specific GHF-1-dependent genes.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Giacomini, M. Paez-Pereda, J. Stalla, G. K. Stalla, and E. Arzt Molecular Interaction of BMP-4, TGF-{beta}, and Estrogens in Lactotrophs: Impact on the PRL Promoter Mol. Endocrinol., July 1, 2009; 23(7): 1102 - 1114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhu, A. S. Gleiberman, and M. G. Rosenfeld Molecular Physiology of Pituitary Development: Signaling and Transcriptional Networks Physiol Rev, July 1, 2007; 87(3): 933 - 963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Duval, M. D. Jonsen, S. E. Diamond, P. Murapa, A. Jean, and A. Gutierrez-Hartmann Differential Utilization of Transcription Activation Subdomains by Distinct Coactivators Regulates Pit-1 Basal and Ras Responsiveness Mol. Endocrinol., January 1, 2007; 21(1): 172 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Liu, R. E. Baker, W. Chow, C. K. Sun, and H. P. Elsholtz Epigenetic Mechanisms in the Dopamine D2 Receptor-Dependent Inhibition of the Prolactin Gene Mol. Endocrinol., July 1, 2005; 19(7): 1904 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A L Ferry, D M Locasto, L B Meszaros, J C Bailey, M D Jonsen, K Brodsky, C J Hoon, A Gutierrez-Hartmann, and S E Diamond Pit-1{beta} reduces transcription and CREB-binding protein recruitment in a DNA context-dependent manner J. Endocrinol., April 1, 2005; 185(1): 173 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Duval, A. Jean, and A. Gutierrez-Hartmann Ras Signaling and Transcriptional Synergy at a Flexible Ets-1/Pit-1 Composite DNA Element Is Defined by the Assembly of Selective Activation Domains J. Biol. Chem., October 10, 2003; 278(41): 39684 - 39696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Schweppe, A. A. Melton, K. S. Brodsky, L. D. Aveline, K. A. Resing, N. G. Ahn, and A. Gutierrez-Hartmann Purification and Mass Spectrometric Identification of GA-binding Protein (GABP) as the Functional Pituitary Ets Factor Binding to the Basal Transcription Element of the Prolactin Promoter J. Biol. Chem., May 2, 2003; 278(19): 16863 - 16872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Augustijn, D. L. Duval, R. Wechselberger, R. Kaptein, A. Gutierrez-Hartmann, and P. C. van der Vliet Structural characterization of the PIT-1/ETS-1 interaction: PIT-1 phosphorylation regulates PIT-1/ETS-1 binding PNAS, October 1, 2002; 99(20): 12657 - 12662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Liu, R. E. Baker, C. Sun, V. C. Sundmark, and H. P. Elsholtz Activation of Go-coupled Dopamine D2 Receptors Inhibits ERK1/ERK2 in Pituitary Cells. A KEY STEP IN THE TRANSCRIPTIONAL SUPPRESSION OF THE PROLACTIN GENE J. Biol. Chem., September 20, 2002; 277(39): 35819 - 35825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kanasaki, T. Yonehara, H. Yamamoto, Y. Takeuchi, K. Fukunaga, K. Takahashi, K. Miyazaki, and E. Miyamoto Differential Regulation of Pituitary Hormone Secretion and Gene Expression by Thyrotropin-Releasing Hormone. A Role for Mitogen-Activated Protein Kinase Signaling Cascade in Rat Pituitary GH3 Cells Biol Reprod, July 1, 2002; 67(1): 107 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Duval and A. Gutierrez-Hartmann Editorial: PRL-Releasing Peptide Stimulation of PRL Gene Transcription--Enter AKT Endocrinology, January 1, 2002; 143(1): 11 - 12. [Full Text] [PDF]
|
|
|
|
|
|
J. Hayakawa, M. Ohmichi, K. Tasaka, Y. Kanda, K. Adachi, Y. Nishio, K. Hisamoto, S. Mabuchi, S. Hinuma, and Y. Murata Regulation of the PRL Promoter by Akt through cAMP Response Element Binding Protein Endocrinology, January 1, 2002; 143(1): 13 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kievit, J. D. Lauten, and R. A. Maurer Analysis of the Role of the Mitogen-Activated Protein Kinase in Mediating Cyclic-Adenosine 3',5'-Monophosphate Effects on Prolactin Promoter Activity Mol. Endocrinol., April 1, 2001; 15(4): 614 - 624. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. E. Schweppe and A. Gutierrez-Hartmann Pituitary Ets-1 and GABP bind to the growth factor regulatory sites of the rat prolactin promoter Nucleic Acids Res., March 1, 2001; 29(5): 1251 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Andersen and M. G. Rosenfeld POU Domain Factors in the Neuroendocrine System: Lessons from Developmental Biology Provide Insights into Human Disease Endocr. Rev., February 1, 2001; 22(1): 2 - 35. [Abstract] [Full Text]
|
|
|
|
|
|
S.-I. Jang, N. Karaman-Jurukovska, M. I. Morasso, P. M. Steinert, and N. G. Markova Complex Interactions between Epidermal POU Domain and Activator Protein 1 Transcription Factors Regulate the Expression of the Profilaggrin Gene in Normal Human Epidermal Keratinocytes J. Biol. Chem., May 12, 2000; 275(20): 15295 - 15304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Bradford, K. S. Brodsky, S. E. Diamond, L. C. Kuhn, Y. Liu, and A. Gutierrez-Hartmann The Pit-1 Homeodomain and beta -Domain Interact with Ets-1 and Modulate Synergistic Activation of the Rat Prolactin Promoter J. Biol. Chem., February 4, 2000; 275(5): 3100 - 3106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kimura, M. Ohmichi, K. Tasaka, Y. Kanda, H. Ikegami, J. Hayakawa, K. Hisamoto, K.-i. Morishige, S. Hinuma, H. Kurachi, et al. Prolactin-releasing Peptide Activation of the Prolactin Promoter Is Differentially Mediated by Extracellular Signal-regulated Protein Kinase and c-Jun N-terminal Protein Kinase J. Biol. Chem., February 4, 2000; 275(5): 3667 - 3674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Jacob and F. M. Stanley CCAAT/Enhancer-Binding Protein {alpha} Is a Physiological Regulator of Prolactin Gene Expression Endocrinology, October 1, 1999; 140(10): 4542 - 4550. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-H. Wang and R. A. Maurer A Role for the Mitogen-Activated Protein Kinase in Mediating the Ability of Thyrotropin-Releasing Hormone to Stimulate the Prolactin Promoter Mol. Endocrinol., July 1, 1999; 13(7): 1094 - 1104. [Abstract] [Full Text]
|
|
|
|
|
|
H. Kanasaki, K. Fukunaga, K. Takahashi, K. Miyazaki, and E. Miyamoto Mitogen-Activated Protein Kinase Activation by Stimulation with Thyrotropin-Releasing Hormone in Rat Pituitary GH3 Cells Biol Reprod, July 1, 1999; 61(1): 319 - 325. [Abstract] [Full Text]
|
|
|
|
|
|
M. S. Fliss, P. M. Hinkle, and C. Bancroft Expression Cloning and Characterization of PREB (Prolactin Regulatory Element Binding), a Novel WD Motif DNA-Binding Protein with a Capacity to Regulate Prolactin Promoter Activity Mol. Endocrinol., April 1, 1999; 13(4): 644 - 657. [Abstract] [Full Text]
|
|
|
|
|
|
S. E. Diamond, M. Chiono, and A. Gutierrez-Hartmann Reconstitution of the Protein Kinase A Response of the Rat Prolactin Promoter: Differential Effects of Distinct Pit-1 Isoforms and Functional Interaction with Oct-1 Mol. Endocrinol., February 1, 1999; 13(2): 228 - 238. [Abstract] [Full Text]
|
|
|
|
|
|
R. N. Day, J. Liu, V. Sundmark, M. Kawecki, D. Berry, and H. P. Elsholtz Selective Inhibition of Prolactin Gene Transcription by the ETS-2 Repressor Factor J. Biol. Chem., November 27, 1998; 273(48): 31909 - 31915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. N. Yager, H.-O. Lee, D. L. Nagle, and J. E. Zimmerman Analysis of fluG Mutations That Affect Light-Dependent Conidiation in Aspergillus nidulans Genetics, August 1, 1998; 149(4): 1777 - 1786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yang, L. H. Shapiro, M. Rivera, A. Kumar, and P. K. Brindle A Role for CREB Binding Protein and p300 Transcriptional Coactivators in Ets-1 Transactivation Functions Mol. Cell. Biol., April 1, 1998; 18(4): 2218 - 2229. [Abstract] [Full Text]
|
|
|
|
|
|
R. E. Schweppe, A. A. Frazer-Abel, A. Gutierrez-Hartmann, and A. P. Bradford Functional Components of Fibroblast Growth Factor (FGF) Signal Transduction in Pituitary Cells. IDENTIFICATION OF FGF RESPONSE ELEMENTS IN THE PROLACTIN GENE J. Biol. Chem., December 5, 1997; 272(49): 30852 - 30859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Gordon, S. R. Lewis, B. R. Haugen, R. A. James, M. T. McDermott, W. M. Wood, and E. C. Ridgway Pit-1 and GATA-2 Interact and Functionally Cooperate to Activate the Thyrotropin beta -Subunit Promoter J. Biol. Chem., September 26, 1997; 272(39): 24339 - 24347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Diamond and A. Gutierrez-Hartmann The Pit-1beta Domain Dictates Active Repression and Alteration of Histone Acetylation of the Proximal Prolactin Promoter J. Biol. Chem., September 29, 2000; 275(40): 30977 - 30986. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |