HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by De, B. P. Articles by Banerjee, AmiyaK. Search for Related Content PubMed PubMed Citation Articles by De, B. P. Articles by Banerjee, AmiyaK. Social Bookmarking                      What's this?

Volume 271, Number 40, Issue of October 4, 1996 pp. 24728-24735
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Specific Interaction in Vitro and in Vivo of Glyceraldehyde-3-phosphate Dehydrogenase and LA Protein with Cis-acting RNAs of Human Parainfluenza Virus Type 3

(Received for publication, March 27, 1996, and in revised form, July 15, 1996)

From the Departments of Molecular Biology and ^§ Neurosciences, Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195

Human parainfluenza virus type 3 (HPIV3) genome RNA is transcribed and replicated by the virus-encoded RNA-dependent RNA polymerase, and specific cellular proteins play a regulatory role in these processes. To search for cellular proteins potentially interacting with HPIV3 cis-acting regulatory RNAs, a gel mobility shift assay was used. Two cellular proteins specifically interacted with the viral cis-acting RNAs containing the genomic 3-noncoding region and the plus-sense leader sequence region. Surprisingly, by biochemical and immunological analyses, one of the cellular proteins was identified as the key glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The other protein was characterized as the autoantigen, LA protein. Both GAPDH and LA protein also interacted with the same cis-acting RNA sequences in vivo and were found to be associated with the HPIV3 ribonucleoprotein complex in the infected cells. By double immunofluorescent labeling, GAPDH was found to be co-localized with viral ribonucleoprotein in the perinuclear region. These observations strongly suggest that cellular GAPDH and LA Protein participate in the regulation of HPIV3 gene expression.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Rodriguez-Pascual, M. Redondo-Horcajo, N. Magan-Marchal, D. Lagares, A. Martinez-Ruiz, H. Kleinert, and S. Lamas Glyceraldehyde-3-Phosphate Dehydrogenase Regulates Endothelin-1 Expression by a Novel, Redox-Sensitive Mechanism Involving mRNA Stability Mol. Cell. Biol., December 1, 2008; 28(23): 7139 - 7155. [Abstract] [Full Text] [PDF]

				V. Bitko, A. Musiyenko, M. A. Bayfield, R. J. Maraia, and S. Barik Cellular La Protein Shields Nonsegmented Negative-Strand RNA Viral Leader RNA from RIG-I and Enhances Virus Growth by Diverse Mechanisms J. Virol., August 15, 2008; 82(16): 7977 - 7987. [Abstract] [Full Text] [PDF]

				Y. Zhou, X. Yi, J. B. Stoffer, N. Bonafe, M. Gilmore-Hebert, J. McAlpine, and S. K. Chambers The Multifunctional Protein Glyceraldehyde-3-Phosphate Dehydrogenase Is Both Regulated and Controls Colony-Stimulating Factor-1 Messenger RNA Stability in Ovarian Cancer Mol. Cancer Res., August 1, 2008; 6(8): 1375 - 1384. [Abstract] [Full Text] [PDF]

				P. Walpita and C. J. Peters Cis-acting elements in the antigenomic promoter of Nipah virus J. Gen. Virol., September 1, 2007; 88(9): 2542 - 2551. [Abstract] [Full Text] [PDF]

				M. P. Bachmann, H. Bartsch, J. K. Gross, S. M. Maier, T. F. Gross, J. L. Workman, J. A. James, A. D. Farris, B. Jung, C. Franke, et al. Autoimmunity as a Result of Escape from RNA Surveillance J. Immunol., August 1, 2006; 177(3): 1698 - 1707. [Abstract] [Full Text] [PDF]

				R. M. E. Yocupicio-Monroy, F. Medina, J. Reyes-del Valle, and R. M. del Angel Cellular Proteins from Human Monocytes Bind to Dengue 4 Virus Minus-Strand 3' Untranslated Region RNA J. Virol., March 1, 2003; 77(5): 3067 - 3076. [Abstract] [Full Text] [PDF]

				G. Dollenmaier and M. Weitz Interaction of glyceraldehyde-3-phosphate dehydrogenase with secondary and tertiary RNA structural elements of the hepatitis A virus 3' translated and non-translated regions J. Gen. Virol., January 1, 2003; 84(2): 403 - 414. [Abstract] [Full Text] [PDF]

				E. Evguenieva-Hackenberg, E. Schiltz, and G. Klug Dehydrogenases from All Three Domains of Life Cleave RNA J. Biol. Chem., November 22, 2002; 277(48): 46145 - 46150. [Abstract] [Full Text] [PDF]

				Y. Fujii, T. Sakaguchi, K. Kiyotani, C. Huang, N. Fukuhara, Y. Egi, and T. Yoshida Involvement of the Leader Sequence in Sendai Virus Pathogenesis Revealed by Recovery of a Pathogenic Field Isolate from cDNA J. Virol., July 29, 2002; 76(17): 8540 - 8547. [Abstract] [Full Text] [PDF]

				M. Huarte, J. J. Sanz-Ezquerro, F. Roncal, J. Ortin, and A. Nieto PA Subunit from Influenza Virus Polymerase Complex Interacts with a Cellular Protein with Homology to a Family of Transcriptional Activators J. Virol., September 15, 2001; 75(18): 8597 - 8604. [Abstract] [Full Text] [PDF]

				M. Yi, D. E. Schultz, and S. M. Lemon Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function J. Virol., July 15, 2000; 74(14): 6459 - 6468. [Abstract] [Full Text]

				S. Choudhary, B. P. De, and A. K. Banerjee Specific Phosphorylated Forms of Glyceraldehyde 3-Phosphate Dehydrogenase Associate with Human Parainfluenza Virus Type 3 and Inhibit Viral Transcription In Vitro J. Virol., April 15, 2000; 74(8): 3634 - 3641. [Abstract] [Full Text]

				T. Ogino, M. Iwama, J. Kinouchi, Y. Shibagaki, T. Tsukamoto, and K. Mizumoto Involvement of a Cellular Glycolytic Enzyme, Phosphoglycerate Kinase, in Sendai Virus Transcription J. Biol. Chem., December 10, 1999; 274(50): 35999 - 36008. [Abstract] [Full Text] [PDF]

				J. Petrik, H. Parker, and G. J. M. Alexander Human hepatic glyceraldehyde-3-phosphate dehydrogenase binds to the poly(U) tract of the 3' non-coding region of hepatitis C virus genomic RNA J. Gen. Virol., December 1, 1999; 80(12): 3109 - 3113. [Abstract] [Full Text]

				M.-H. Chen and T. K. Frey Mutagenic Analysis of the 3' cis-Acting Elements of the Rubella Virus Genome J. Virol., April 1, 1999; 73(4): 3386 - 3403. [Abstract] [Full Text]

				A. K. Gupta, J. A. Drazba, and A. K. Banerjee Specific Interaction of Heterogeneous Nuclear Ribonucleoprotein Particle U with the Leader RNA Sequence of Vesicular Stomatitis Virus J. Virol., November 1, 1998; 72(11): 8532 - 8540. [Abstract] [Full Text] [PDF]

				M. Engel, M. Seifert, B. Theisinger, U. Seyfert, and C. Welter Glyceraldehyde-3-phosphate Dehydrogenase and Nm23-H1/Nucleoside Diphosphate Kinase A. TWO OLD ENZYMES COMBINE FOR THE NOVEL Nm23 PROTEIN PHOSPHOTRANSFERASE FUNCTION J. Biol. Chem., August 7, 1998; 273(32): 20058 - 20065. [Abstract] [Full Text] [PDF]

				S. Gupta, B. P. De, J. A. Drazba, and A. K. Banerjee Involvement of Actin Microfilaments in the Replication of Human Parainfluenza Virus Type 3 J. Virol., April 1, 1998; 72(4): 2655 - 2662. [Abstract] [Full Text] [PDF]

				K. Ranganna and F. M. Yatsu Inhibition of Platelet-Derived Growth Factor BB–Induced Expression of Glyceraldehyde- 3-Phosphate Dehydrogenase by Sodium Butyrate in Rat Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3420 - 3427. [Abstract] [Full Text]