HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pallante, K. M. Articles by Adams, S. L. Search for Related Content PubMed PubMed Citation Articles by Pallante, K. M. Articles by Adams, S. L. Social Bookmarking                      What's this?

Volume 271, Number 41, Issue of October 11, 1996 pp. 25233-25239
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

The Chick 2(I) Collagen Gene Contains Two Functional Promoters, and Its Expression in Chondrocytes Is Regulated at Both Transcriptional and Post-transcriptional Levels

(Received for publication, April 25, 1996, and in revised form, July 3, 1996)

From the Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6003

Embryonic chick cartilages contain transcripts derived from the 2(I) collagen gene, although type I collagen is not normally found in these tissues; most of these RNAs are alternative transcripts initiating within intron 2. Use of the internal start site results in replacement of exons 1 and 2 with a previously undescribed exon and a change in the translational reading frame; thus, the alternative transcript cannot encode 2(I) collagen. We have demonstrated that production of the alternative transcript is due to activation of an internal promoter in chondrocytes and have identified a 179-base pair domain that is required for its activity. Furthermore, we have shown that the alternative transcript resulting from activation of the internal promoter turns over relatively rapidly; thus, the steady-state level of this transcript is less than predicted based on the transcription rate. The upstream promoter is only partially repressed in chondrocytes, suggesting that the lack of authentic 2(I) collagen mRNA may also be due in part to decreased mRNA stability. Thus, repression of 2(I) collagen synthesis in cartilage involves both transcriptional and post-transcriptional mechanisms. In contrast, repression of 1(I) collagen synthesis appears to be mediated primarily at the level of transcription.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J.-J. Wu and D. R. Eyre Intervertebral Disc Collagen: USAGE OF THE SHORT FORM OF THE {alpha}1(IX) CHAIN IN BOVINE NUCLEUS PULPOSUS J. Biol. Chem., June 27, 2003; 278(27): 24521 - 24525. [Abstract] [Full Text] [PDF]

				D. P. Giedroc, X. Chen, M. A. Pennella, and A. C. LiWang Conformational Heterogeneity in the C-terminal Zinc Fingers of Human MTF-1. AN NMR AND ZINC-BINDING STUDY J. Biol. Chem., November 2, 2001; 276(45): 42322 - 42332. [Abstract] [Full Text] [PDF]