HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, Q. Articles by Fisher, A. B. Search for Related Content PubMed PubMed Citation Articles by Chen, Q. Articles by Fisher, A. B. Social Bookmarking                      What's this?

Volume 271, Number 41, Issue of October 11, 1996 pp. 25277-25283
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Secretagogues Increase the Expression of Surfactant Protein A Receptors on Lung Type II Cells

(Received for publication, January 26, 1996, and in revised form, May 15, 1996)

From the Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6068

Since secretagogues have been shown to increase the internalization of surfactant phospholipid and protein by lung cells, we postulated that their action occurred through a mechanism involving increased surfactant protein A (SP-A) receptor density. Therefore, we evaluated the influence of secretagogues on the binding of iodinated SP-A to alveolar type II cells. Type II cells were isolated from rat lung and maintained in primary culture for 18 h on Transwell membranes. Upon exposure to 8-bromo-cyclic AMP (cAMP, 0.1 mM), phorbol 12-myristate 13-acetate (PMA, 10 nM), terbutaline (0.1 mM), or ATP (1 mM), the binding of SP-A increased 1.5-2-fold. This stimulation was cell substrate-dependent since type II cells plated on plastic dishes did not show this effect. A time course of the stimulation of SP-A binding due to secretagogues showed that both cAMP and PMA increased SP-A binding by 2-fold after 20 min. With cAMP, binding remained elevated for 2 h, while binding in the presence of PMA had returned to control values. The effects of submaximal concentrations of cAMP and PMA on binding were additive. Inhibition of cellular protein synthesis with cycloheximide did not alter the increase of SP-A binding stimulated by the secretagogues. Type II cells pretreated with PMA responded to subsequent treatment with cAMP by increasing SP-A binding, while these cells were refractory to subsequent treatment with PMA. Both constitutive and regulated binding of SP-A to type II cells were sensitive to trypsin. The binding of SP-A to type II cells showed saturation at a concentration of 1 µg/ml SP-A under control and secretagogue-stimulated conditions, with both total and calcium-dependent binding showing a 2-fold increase upon secretagogue exposure. The data are consistent with the hypothesis that secretagogues stimulate surfactant uptake, at least in part, through recruitment of SP-A receptors to the type II cell surface, resulting in an increase in the number of SP-A binding sites.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. R. Bates, A. S. Kazi, J.-Q. Tao, K. J. Yu, D. S. Gonder, S. I. Feinstein, and A. B. Fisher Role of P63 (CKAP4) in binding of surfactant protein-A to type II pneumocytes Am J Physiol Lung Cell Mol Physiol, October 1, 2008; 295(4): L658 - L669. [Abstract] [Full Text] [PDF]

				G. M. Mutlu and P. Factor Alveolar Epithelial 2-Adrenergic Receptors Am. J. Respir. Cell Mol. Biol., February 1, 2008; 38(2): 127 - 134. [Abstract] [Full Text] [PDF]

				S. R. Bates, C. Dodia, J.-Q. Tao, and A. B. Fisher Surfactant protein-A plays an important role in lung surfactant clearance: evidence using the surfactant protein-A gene-targeted mouse Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L325 - L333. [Abstract] [Full Text] [PDF]

				D. Jain, C. Dodia, A. B. Fisher, and S. R. Bates Pathways for clearance of surfactant protein A from the lung Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1011 - L1018. [Abstract] [Full Text] [PDF]

				S. R. Bates, J.-Q. Tao, K. Notarfrancesco, K. DeBolt, H. Shuman, and A. B. Fisher Effect of surfactant protein A on granular pneumocyte surfactant secretion in vitro Am J Physiol Lung Cell Mol Physiol, November 1, 2003; 285(5): L1055 - L1065. [Abstract] [Full Text] [PDF]

				D. Jain, C. Dodia, S. R. Bates, S. Hawgood, F. R. Poulain, and A. B. Fisher SP-A is necessary for increased clearance of alveolar DPPC with hyperventilation or secretagogues Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L759 - L765. [Abstract] [Full Text] [PDF]

				A. B. Fisher and C. Dodia Lysosomal-type PLA2 and turnover of alveolar DPPC Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L748 - L754. [Abstract] [Full Text] [PDF]

				S. R. Bates, J.-Q. Tao, S. Schaller, A. B. Fisher, and H. Shuman Lamellar body membrane turnover is stimulated by secretagogues Am J Physiol Lung Cell Mol Physiol, March 1, 2000; 278(3): L443 - L452. [Abstract] [Full Text] [PDF]

				P. L Shah, D. Hansell, P. R Lawson, K. B M Reid, and C. Morgan Rare diseases bullet 6: Pulmonary alveolar proteinosis: clinical aspects and current concepts on pathogenesis Thorax, January 1, 2000; 55(1): 67 - 77. [Full Text]

				Q. Chen, A. B. Fisher, D. S. Strayer, and S. R. Bates Mechanism for secretagogue-induced surfactant protein A binding to lung epithelial cells Am J Physiol Lung Cell Mol Physiol, July 1, 1998; 275(1): L38 - L46. [Abstract] [Full Text] [PDF]

				K. Zen, K. Notarfrancesco, V. Oorschot, J. W. Slot, A. B. Fisher, and H. Shuman Generation and characterization of monoclonal antibodies to alveolar type II cell lamellar body membrane Am J Physiol Lung Cell Mol Physiol, July 1, 1998; 275(1): L172 - L183. [Abstract] [Full Text] [PDF]

				A. J. Gow, S. R. Thom, and H. Ischiropoulos Nitric oxide and peroxynitrite-mediated pulmonary cell death Am J Physiol Lung Cell Mol Physiol, January 1, 1998; 274(1): L112 - L118. [Abstract] [Full Text] [PDF]

				I. R. DOYLE, K. G. DAVIDSON, H. A. BARR, T. E. NICHOLAS, K. PAYNE, and J. PFITZNER Quantity and Structure of Surfactant Proteins Vary Among Patients with Alveolar Proteinosis Am. J. Respir. Crit. Care Med., February 1, 1997; 157(2): 658 - 664. [Abstract] [Full Text]

				S. R. Bates, L. W. Gonzales, J.-Q. Tao, P. Rueckert, P. L. Ballard, and A. B. Fisher Recovery of rat type II cell surfactant components during primary cell culture Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L267 - L276. [Abstract] [Full Text] [PDF]