HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Akasawa, A. Articles by Lin, Y. Search for Related Content PubMed PubMed Citation Articles by Akasawa, A. Articles by Lin, Y. Social Bookmarking                      What's this?

Volume 271, Number 41, Issue of October 11, 1996 pp. 25389-25393
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

A Novel Acidic Allergen, Hev b 5, in Latex
PURIFICATION, CLONING AND CHARACTERIZATION

(Received for publication, April 5, 1996, and in revised form, July 24, 1996)

From the Division of Allergenic Products and Parasitology, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20852 and the ^¶ Unit on Molecular Structures, Clinical Neuroscience Branch, National Institute of Mental Health, Bethesda, Maryland 20892

Latex allergy is recognized as a serious health problem among health care workers and children with spina bifida. A number of IgE-reactive proteins have been identified in natural and processed latex products. One of the most acidic proteins in the cytoplasm of lacticifer cells of rubber trees (Hevea brasiliensis) is demonstrated to be a potent allergen in eliciting allergic reactions in humans. This protein, with pI = 3.5, has a molecular mass of 16 kDa with a blocked N terminus and an unusual amino acid composition. This acidic protein was found in extracts prepared from latex gloves, which were shown to be allergenic. The purified protein elicits histamine release from human basophils passively sensitized with serum from latex-allergic individuals in a dose-dependent manner.

From a latex cDNA library, the cDNA coding for this protein was isolated and sequenced. The deduced amino acid sequence shows a high degree of homology to another acidic protein identified in kiwifruit (Actinidia deliciosa var. deliciosa). The sequence homology (47% sequence identity) between these two acidic proteins suggests a molecular explanation for the high frequency of fruit hypersensitivity in latex-allergic patients.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. R. Nordlund, V. P. Hytonen, O. H. Laitinen, and M. S. Kulomaa Novel Avidin-like Protein from a Root Nodule Symbiotic Bacterium, Bradyrhizobium japonicum J. Biol. Chem., April 8, 2005; 280(14): 13250 - 13255. [Abstract] [Full Text] [PDF]

				C. L. Hardy, L. Kenins, A. C. Drew, J. M. Rolland, and R. E. O'Hehir Characterization of a Mouse Model of Allergy to a Major Occupational Latex Glove Allergen Hev b 5 Am. J. Respir. Crit. Care Med., May 15, 2003; 167(10): 1393 - 1399. [Abstract] [Full Text] [PDF]

				H Alenius, K Turjanmaa, and T Palosuo Natural rubber latex allergy Occup. Environ. Med., June 1, 2002; 59(6): 419 - 424. [Full Text] [PDF]

				M. R. Fowler, J. Gartland, W. Norton, A. Slater, M. C. Elliott, and N. W. Scott RS2: a sugar beet gene related to the latex allergen Hev b 5 family J. Exp. Bot., December 1, 2000; 51(353): 2125 - 2126. [Abstract] [Full Text] [PDF]

				S. K. Oh, H. Kang, D. H. Shin, J. Yang, K.-S. Chow, H. Y. Yeang, B. Wagner, H. Breiteneder, and K.-H. Han Isolation, Characterization, and Functional Analysis of a Novel cDNA Clone Encoding a Small Rubber Particle Protein from Hevea brasiliensis J. Biol. Chem., June 11, 1999; 274(24): 17132 - 17138. [Abstract] [Full Text] [PDF]