Identification of the cpdA Gene Encoding Cyclic 3',5'-Adenosine Monophosphate Phosphodiesterase in Escherichia coli

doi:10.1074/jbc.271.41.25423

HOME

HELP

FEEDBACK

SUBSCRIPTIONS

Volume 271, Number 41, Issue of October 11, 1996 pp. 25423-25429
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

Identification of the cpdA Gene Encoding Cyclic 3 $'$ ,5 $'$ -Adenosine Monophosphate Phosphodiesterase in Escherichia coli

(Received for publication, November 13, 1995, and in revised form, June 21, 1996)

Ryu Imamura , Kunitoshi Yamanaka , Teru Ogura , Sota Hiraga , Nobuyuki Fujita ^§ , Akira Ishihama ^§ and Hironori Niki

From the Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto, Kumamoto 862 and the ^§ Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan

We have identified a gene, cpdA, located at 66.2 min of the chromosome of Escherichia coli that encodes cyclic 3 $'$ ,5 $'$ -adenosine monophosphate phosphodiesterase (cAMP phosphodiesterase, EC). The expression of $beta$ -galactosidase, which is a product of the lacZ gene, was repressed in cells that harbored multiple copies of the plasmid carrying the cpdA gene. Northern blotting showed that the transcription of the lacZ gene was inhibited in these cells. Multiple copies of the cpdA gene decreased the intracellular concentration of cAMP, which is a positive regulator for transcription of the lacZ gene. We found that the purified CpdA protein repressed in vitro transcription from the lacP1 promoter by decreasing cAMP. In addition, we showed that the CpdA protein hydrolyzed cAMP to 5 $'$ -adenosine monophosphate and that its activity was activated by iron. Our results suggested that regulation of intracellular concentration of cAMP is dependent not only on synthesis of cAMP but also on hydrolysis of cAMP by cAMP phosphodiesterase.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

E. Barth, K. V. Gora, K. M. Gebendorfer, F. Settele, U. Jakob, and J. Winter
Interplay of cellular cAMP levels, {sigma}S activity and oxidative stress resistance in Escherichia coli
Microbiology, May 1, 2009; 155(5): 1680 - 1689.
[Abstract] [Full Text] [PDF]

H.-S. Kim, S.-M. Kim, H.-J. Lee, S.-J. Park, and K.-H. Lee
Expression of the cpdA Gene, Encoding a 3',5'-Cyclic AMP (cAMP) Phosphodiesterase, Is Positively Regulated by the cAMP-cAMP Receptor Protein Complex
J. Bacteriol., February 1, 2009; 191(3): 922 - 930.
[Abstract] [Full Text] [PDF]

T. Kuhlman, Z. Zhang, M. H. Saier Jr., and T. Hwa
Combinatorial transcriptional control of the lactose operon of Escherichia coli
PNAS, April 3, 2007; 104(14): 6043 - 6048.
[Abstract] [Full Text] [PDF]

Y. Kimura, H. Nakatuma, N. Sato, and M. Ohtani
Contribution of the Cyclic Nucleotide Phosphodiesterases PdeA and PdeB to Adaptation of Myxococcus xanthus Cells to Osmotic or High-Temperature Stress
J. Bacteriol., January 15, 2006; 188(2): 823 - 828.
[Abstract] [Full Text] [PDF]

T. Yoshimura-Suzuki, I. Sagami, N. Yokota, H. Kurokawa, and T. Shimizu
DOSEc, a Heme-Regulated Phosphodiesterase, Plays an Important Role in the Regulation of the Cyclic AMP Level in Escherichia coli
J. Bacteriol., October 1, 2005; 187(19): 6678 - 6682.
[Abstract] [Full Text] [PDF]

R. Tamayo, A. D. Tischler, and A. Camilli
The EAL Domain Protein VieA Is a Cyclic Diguanylate Phosphodiesterase
J. Biol. Chem., September 30, 2005; 280(39): 33324 - 33330.
[Abstract] [Full Text] [PDF]

S. K. Tehara and J. D. Keasling
Gene Cloning, Purification, and Characterization of a Phosphodiesterase from Delftia acidovorans
Appl. Envir. Microbiol., January 1, 2003; 69(1): 504 - 508.
[Abstract] [Full Text] [PDF]

D. Li, H. Zhu, K. Liu, X. Liu, G. Leggewie, M. Udvardi, and D. Wang
Purple Acid Phosphatases of Arabidopsis thaliana. COMPARATIVE ANALYSIS AND DIFFERENTIAL REGULATION BY PHOSPHATE DEPRIVATION
J. Biol. Chem., July 26, 2002; 277(31): 27772 - 27781.
[Abstract] [Full Text] [PDF]

Y. Sasakura, S. Hirata, S. Sugiyama, S. Suzuki, S. Taguchi, M. Watanabe, T. Matsui, I. Sagami, and T. Shimizu
Characterization of a Direct Oxygen Sensor Heme Protein from Escherichia coli. EFFECTS OF THE HEME REDOX STATES AND MUTATIONS AT THE HEME-BINDING SITE ON CATALYSIS AND STRUCTURE
J. Biol. Chem., June 21, 2002; 277(26): 23821 - 23827.
[Abstract] [Full Text] [PDF]

S. Campoy, M. Jara, N. Busquets, A. M. P. de Rozas, I. Badiola, and J. Barbe
Intracellular cyclic AMP concentration is decreased in Salmonella typhimurium fur mutants
Microbiology, April 1, 2002; 148(4): 1039 - 1048.
[Abstract] [Full Text] [PDF]

M. J. Soto, J. I. Jiménez-Zurdo, P. van Dillewijn, and N. Toro
Sinorhizobium meliloti putA Gene Regulation: a New Model within the Family Rhizobiaceae
J. Bacteriol., April 1, 2000; 182(7): 1935 - 1941.
[Abstract] [Full Text]

M. K.�B. Berlyn
Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map
Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984.
[Abstract] [Full Text] [PDF]

L. P. Macfadyen, C. Ma, and R. J. Redfield
A 3',5' Cyclic AMP (cAMP) Phosphodiesterase Modulates cAMP Levels and Optimizes Competence in Haemophilus influenzae Rd
J. Bacteriol., September 1, 1998; 180(17): 4401 - 4405.
[Abstract] [Full Text]

M. Yoshida, K. Kashiwagi, G. Kawai, A. Ishihama, and K. Igarashi
Polyamine Enhancement of the Synthesis of Adenylate Cyclase at the Translational Level and the Consequential Stimulation of the Synthesis of the RNA Polymerase sigma 28 Subunit
J. Biol. Chem., May 4, 2001; 276(19): 16289 - 16295.
[Abstract] [Full Text] [PDF]