| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, April 3, 1996, and in revised form, June 26, 1996)
From the Department of Biochemistry and Cancer Center, University
of Rochester School of Medicine and Dentistry, Rochester, New
York 14642
In eukaryotes, the endonucleolytic activity of
the calf RTH-1 class 5
Volume 271, Number 42,
Issue of October 18, 1996
pp. 25888-25897
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
- to 3-exo/endonuclease can function without
RNase H1 to remove initiator RNA from Okazaki fragments. Cleavage
requires that the RNA be displaced to form an unannealed
single-stranded 5-tail or flap structure. On substrates with
RNA-initiated primers, DNA oligomers that competed with the RNA for
template binding simulated strand displacement synthesis from an
upstream Okazaki fragment. This allowed cutting of displaced RNA
segments by RTH-1 nuclease. Requirements for the reaction also were
examined on substrates in which the tail was unannealed because it was
intentionally mispaired. On both types of substrate, the nuclease
slides over the RNA region from the 5-end and cleaves at the beginning
of the annealed region, irrespective of whether ribo- or
deoxyribonucleotides are at the cleavage site. Presence of a
triphosphate or a 7-methyl 3G5ppp5 G cap structure at the 5-end of
the RNA does not affect cleavage. The previously reported stimulation
of the nuclease by an upstream primer was not always observed,
suggesting that not every site in the downstream Okazaki fragment is
equally susceptible to cleavage during displacement synthesis in
vivo. The biological role of the endonuclease activity of RTH-1
nuclease in Okazaki fragment processing is discussed.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. M. Stith, J. Sterling, M. A. Resnick, D. A. Gordenin, and P. M. Burgers Flexibility of Eukaryotic Okazaki Fragment Maturation through Regulated Strand Displacement Synthesis J. Biol. Chem., December 5, 2008; 283(49): 34129 - 34140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-F. Richard, A. Kerrest, and B. Dujon Comparative Genomics and Molecular Dynamics of DNA Repeats in Eukaryotes Microbiol. Mol. Biol. Rev., December 1, 2008; 72(4): 686 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Rossi, J. E. Pike, W. Wang, P. M. J. Burgers, J. L. Campbell, and R. A. Bambara Pif1 Helicase Directs Eukaryotic Okazaki Fragments toward the Two-nuclease Cleavage Pathway for Primer Removal J. Biol. Chem., October 10, 2008; 283(41): 27483 - 27493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Stewart, J. L. Campbell, and R. A. Bambara Flap Endonuclease Disengages Dna2 Helicase/Nuclease from Okazaki Fragment Flaps J. Biol. Chem., December 15, 2006; 281(50): 38565 - 38572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Garg, C. M. Stith, N. Sabouri, E. Johansson, and P. M. Burgers Idling by DNA polymerase {delta} maintains a ligatable nick during lagging-strand DNA replication Genes & Dev., November 15, 2004; 18(22): 2764 - 2773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, H. Zhang, J. Veeraraghavan, R. A. Bambara, and C. H. Freudenreich Saccharomyces cerevisiae Flap Endonuclease 1 Uses Flap Equilibration To Maintain Triplet Repeat Stability Mol. Cell. Biol., May 1, 2004; 24(9): 4049 - 4064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-I Kao, J. Veeraraghavan, P. Polaczek, J. L. Campbell, and R. A. Bambara On the Roles of Saccharomyces cerevisiae Dna2p and Flap Endonuclease 1 in Okazaki Fragment Processing J. Biol. Chem., April 9, 2004; 279(15): 15014 - 15024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Matsui, K. V. Musti, J. Abe, K. Yamasaki, I. Matsui, and K. Harata Molecular Structure and Novel DNA Binding Sites Located in Loops of Flap Endonuclease-1 from Pyrococcus horikoshii J. Biol. Chem., September 27, 2002; 277(40): 37840 - 37847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-I Kao, L. A. Henricksen, Y. Liu, and R. A. Bambara Cleavage Specificity of Saccharomyces cerevisiae Flap Endonuclease 1 Suggests a Double-Flap Structure as the Cellular Substrate J. Biol. Chem., April 19, 2002; 277(17): 14379 - 14389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Maga, G. Villani, V. Tillement, M. Stucki, G. A. Locatelli, I. Frouin, S. Spadari, and U. Hubscher Okazaki fragment processing: Modulation of the strand displacement activity of DNA polymerase delta by the concerted action of replication protein A, proliferating cell nuclear antigen, and flap endonuclease-1 PNAS, November 20, 2001; (2001) 251193198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tom, L. A. Henricksen, and R. A. Bambara Mechanism Whereby Proliferating Cell Nuclear Antigen Stimulates Flap Endonuclease 1 J. Biol. Chem., March 31, 2000; 275(14): 10498 - 10505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yao, J. Hurwitz, and M. O'Donnell Dynamics of beta and Proliferating Cell Nuclear Antigen Sliding Clamps in Traversing DNA Secondary Structure J. Biol. Chem., January 14, 2000; 275(2): 1421 - 1432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Matsui, S. Kawasaki, H. Ishida, K. Ishikawa, Y. Kosugi, H. Kikuchi, Y. Kawarabayashi, and I. Matsui Thermostable Flap Endonuclease from the Archaeon, Pyrococcus horikoshii, Cleaves the Replication Fork-like Structure Endo/Exonucleolytically J. Biol. Chem., June 25, 1999; 274(26): 18297 - 18309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Rumbaugh, L. A. Henricksen, M. S. DeMott, and R. A. Bambara Cleavage of Substrates with Mismatched Nucleotides by Flap Endonuclease-1. IMPLICATIONS FOR MAMMALIAN OKAZAKI FRAGMENT PROCESSING J. Biol. Chem., May 21, 1999; 274(21): 14602 - 14608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Kokoska, L. Stefanovic, A. B. Buermeyer, R. M. Liskay, and T. D. Petes A Mutation of the Yeast Gene Encoding PCNA Destabilizes Both Microsatellite and Minisatellite DNA Sequences Genetics, February 1, 1999; 151(2): 511 - 519. [Abstract] [Full Text]
|
|
|
|
|
|
C.-Y. Kim, B. Shen, M. S. Park, and G. A. Olah Structural Changes Measured by X-ray Scattering from Human Flap Endonuclease-1 Complexed with Mg2+ and Flap DNA Substrate J. Biol. Chem., January 15, 1999; 274(3): 1233 - 1239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Garforth, T. A. Ceska, D. Suck, and J. R. Sayers Mutagenesis of conserved lysine residues in bacteriophage T5 5'-3' exonuclease suggests separate mechanisms of endoand exonucleolytic cleavage PNAS, January 5, 1999; 96(1): 38 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Rumbaugh, G. M. Fuentes, and R. A. Bambara Processing of an HIV Replication Intermediate by the Human DNA Replication Enzyme FEN1 J. Biol. Chem., October 30, 1998; 273(44): 28740 - 28745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Kokoska, L. Stefanovic, H. T. Tran, M. A. Resnick, D. A. Gordenin, and T. D. Petes Destabilization of Yeast Micro- and Minisatellite DNA Sequences by Mutations Affecting a Nuclease Involved in Okazaki Fragment Processing (rad27) and DNA Polymerase delta (pol3-t) Mol. Cell. Biol., May 1, 1998; 18(5): 2779 - 2788. [Abstract] [Full Text]
|
|
|
|
|
|
R. S. Murante, L. A. Henricksen, and R. A. Bambara Junction ribonuclease: An activity in Okazaki fragment processing PNAS, March 3, 1998; 95(5): 2244 - 2249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bhagwat, L. J. Hobbs, and N. G. Nossal The 5'-Exonuclease Activity of Bacteriophage T4 RNase H Is Stimulated by the T4 Gene 32 Single-stranded DNA-binding Protein, but Its Flap Endonuclease Is Inhibited J. Biol. Chem., November 7, 1997; 272(45): 28523 - 28530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Rumbaugh, R. S. Murante, S. Shi, and R. A. Bambara Creation and Removal of Embedded Ribonucleotides in Chromosomal DNA during Mammalian Okazaki Fragment Processing J. Biol. Chem., September 5, 1997; 272(36): 22591 - 22599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Bambara, R. S. Murante, and L. A. Henricksen Enzymes and Reactions at the Eukaryotic DNA Replication Fork J. Biol. Chem., February 21, 1997; 272(8): 4647 - 4650. [Full Text] [PDF]
|
|
|
|
|
|
M. S. DeMott, B. Shen, M. S. Park, R. A. Bambara, and S. Zigman Human RAD2 Homolog 15'- to 3'-Exo/Endonuclease Can Efficiently Excise a Displaced DNA Fragment Containing a 5'-Terminal Abasic Lesion by Endonuclease Activity J. Biol. Chem., November 22, 1996; 271(47): 30068 - 30076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, O. Potapova, A. E. Leschziner, N. D. F. Grindley, and C. M. Joyce Contacts between the 5' Nuclease of DNA Polymerase I and Its DNA Substrate J. Biol. Chem., August 3, 2001; 276(32): 30167 - 30177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Henricksen, S. Tom, Y. Liu, and R. A. Bambara Inhibition of Flap Endonuclease 1 by Flap Secondary Structure and Relevance to Repeat Sequence Expansion J. Biol. Chem., May 26, 2000; 275(22): 16420 - 16427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Maga, G. Villani, V. Tillement, M. Stucki, G. A. Locatelli, I. Frouin, S. Spadari, and U. Hubscher Okazaki fragment processing: Modulation of the strand displacement activity of DNA polymerase delta by the concerted action of replication protein A, proliferating cell nuclear antigen, and flap endonuclease-1 PNAS, December 4, 2001; 98(25): 14298 - 14303. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
