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(Received for publication, May 15, 1996, and in revised form, July 26, 1996)
From the Department of Medicine, Beth Israel Hospital, and Harvard
Medical School, Boston, Massachusetts 02215
We previously identified a B-cell-specific
regulatory element in the immunoglobulin heavy chain (IgH) enhancer,
Volume 271, Number 42,
Issue of October 18, 1996
pp. 26007-26012
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Site
, with striking similarity to binding sites for
ets-related transcription factors. Whereas the ability of
ets-related factors to bind to and transactivate the site has been substantiated, the identification of the particular
member of the ets family responsible for B-cell-specific
regulation of the site has remained controversial. We have used
antibodies specific for individual members of the ets
family to evaluate which ets-related factor in B-cell
nuclear extracts interacts with the IgH site. We present strong
evidence that ELF-1 is highly expressed in B-cells and is one of two
major factors specifically interacting with the murine IgH enhancer site in B-cell nuclear extracts. Binding of ELF-1 correlates with
activity of the site, since mutations abolishing function of also inhibit binding of ELF-1. Furthermore, we demonstrate that ELF-1
can transactivate the IgH enhancer in HeLa cells, suggesting a role for
ELF-1 in B-cell-specific IgH gene expression.
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