HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hoang, B. Articles by Luyten, F. P. Search for Related Content PubMed PubMed Citation Articles by Hoang, B. Articles by Luyten, F. P. Social Bookmarking                      What's this?

Volume 271, Number 42, Issue of October 18, 1996 pp. 26131-26137
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Primary Structure and Tissue Distribution of FRZB, a Novel Protein Related to Drosophila Frizzled, Suggest a Role in Skeletal Morphogenesis

(Received for publication, February 26, 1996, and in revised form, July 15, 1996)

Bang Hoang , Malcolm Moos Jr.^¶ , Slobodan Vukicevic and Frank P. Luyten

From the  Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, ^¶ Laboratory of Developmental Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, and  Department of Anatomy, Medical School of Zagreb, 41000 Zagreb, Croatia

Articular cartilage extracts were prepared to characterize protein fractions with in vivo chondrogenic activity (Chang, S., Hoang, B., Thomas, J. T., Vukicevic, S., Luyten, F. P., Ryba, N. J. P., Kozak, C. A., Reddi, A. H., and Moos, M. (1994) J. Biol. Chem. 269, 28227-28234). Trypsin digestion of highly purified chondrogenic protein fractions allowed the identification of several unique peptides by amino acid sequencing. We discovered a novel cDNA encoding a deduced 36-kDa protein by using degenerate oligonucleotide primers derived from a 30-residue peptide in reverse transcription polymerase chain reactions. Its N-terminal domain showed ~50% amino acid identity to the corresponding region of the Drosophila gene frizzled, which has been implicated in the specification of hair polarity during development. Hydropathy and structural analyses of the open reading frame revealed the presence of a signal peptide and a hydrophobic domain followed by multiple potential serine/threonine phosphorylation sites and a serine-rich C terminus. Cell fractionation studies of primary bovine articular chondrocytes and transfected COS cells suggested that the protein is membrane-associated. In situ hybridization and immunostaining of human embryonic sections demonstrated predominant expression surrounding the chondrifying bone primordia and subsequently in the chondrocytes of the epiphyses in a graded distribution that decreased toward the primary ossification center. Transcripts were present in the craniofacial structures but not in the vertebral bodies. Because it is expressed primarily in the cartilaginous cores of developing long bones during embryonic and fetal development (6-13 weeks) and is homologous to the polarity-determining gene frizzled, we believe that this gene, which we named frzb, is involved in morphogenesis of the mammalian skeleton.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. T. Thomas, P. Canelos, F. P. Luyten, and M. Moos Jr. Xenopus SMOC-1 Inhibits Bone Morphogenetic Protein Signaling Downstream of Receptor Binding and Is Essential for Postgastrulation Development in Xenopus J. Biol. Chem., July 10, 2009; 284(28): 18994 - 19005. [Abstract] [Full Text] [PDF]

				H.-S. Kwon, H.-S. Lee, Y. Ji, J. S. Rubin, and S. I. Tomarev Myocilin Is a Modulator of Wnt Signaling Mol. Cell. Biol., April 15, 2009; 29(8): 2139 - 2154. [Abstract] [Full Text] [PDF]

				J. J. Pinzone, B. M. Hall, N. K. Thudi, M. Vonau, Y.-W. Qiang, T. J. Rosol, and J. D. Shaughnessy Jr The role of Dickkopf-1 in bone development, homeostasis, and disease Blood, January 15, 2009; 113(3): 517 - 525. [Abstract] [Full Text] [PDF]

				Y. Guo, J. Xie, E. Rubin, Y.-X. Tang, F. Lin, X. Zi, and B. H. Hoang Frzb, a Secreted Wnt Antagonist, Decreases Growth and Invasiveness of Fibrosarcoma Cells Associated with Inhibition of Met Signaling Cancer Res., May 1, 2008; 68(9): 3350 - 3360. [Abstract] [Full Text] [PDF]

				H K Datta, W F Ng, J A Walker, S P Tuck, and S S Varanasi The cell biology of bone metabolism J. Clin. Pathol., May 1, 2008; 61(5): 577 - 587. [Abstract] [Full Text] [PDF]

				P. Bovolenta, P. Esteve, J. M. Ruiz, E. Cisneros, and J. Lopez-Rios Beyond Wnt inhibition: new functions of secreted Frizzled-related proteins in development and disease J. Cell Sci., March 15, 2008; 121(6): 737 - 746. [Abstract] [Full Text] [PDF]

				X. Zi, Y. Guo, A. R. Simoneau, C. Hope, J. Xie, R. F. Holcombe, and B. H. Hoang Expression of Frzb/Secreted Frizzled-Related Protein 3, a Secreted Wnt Antagonist, in Human Androgen-Independent Prostate Cancer PC-3 Cells Suppresses Tumor Growth and Cellular Invasiveness Cancer Res., November 1, 2005; 65(21): 9762 - 9770. [Abstract] [Full Text] [PDF]

				Y. Nakamura, M. Nawata, and S. Wakitani Expression Profiles and Functional Analyses of Wnt-Related Genes in Human Joint Disorders Am. J. Pathol., July 1, 2005; 167(1): 97 - 105. [Abstract] [Full Text] [PDF]

				J. Loughlin, B. Dowling, K. Chapman, L. Marcelline, Z. Mustafa, L. Southam, A. Ferreira, C. Ciesielski, D. A. Carson, and M. Corr Functional variants within the secreted frizzled-related protein 3 gene are associated with hip osteoarthritis in females PNAS, June 29, 2004; 101(26): 9757 - 9762. [Abstract] [Full Text] [PDF]

				Y. Kawano and R. Kypta Secreted antagonists of the Wnt signalling pathway J. Cell Sci., July 1, 2003; 116(13): 2627 - 2634. [Abstract] [Full Text] [PDF]

				V. Church, T. Nohno, C. Linker, C. Marcelle, and P. Francis-West Wnt regulation of chondrocyte differentiation J. Cell Sci., March 14, 2003; 115(24): 4809 - 4818. [Abstract] [Full Text] [PDF]

				J. De Vos, G. Couderc, K. Tarte, M. Jourdan, G. Requirand, M.-C. Delteil, J.-F. Rossi, N. Mechti, and B. Klein Identifying intercellular signaling genes expressed in malignant plasma cells by using complementary DNA arrays Blood, August 1, 2001; 98(3): 771 - 780. [Abstract] [Full Text] [PDF]

				T Ishikawa, Y Tamai, A. Zorn, H Yoshida, M. Seldin, S Nishikawa, and M. Taketo Mouse Wnt receptor gene Fzd5 is essential for yolk sac and placental angiogenesis Development, January 1, 2001; 128(1): 25 - 33. [Abstract] [PDF]

				A. Uren, F. Reichsman, V. Anest, W. G. Taylor, K. Muraiso, D. P. Bottaro, S. Cumberledge, and J. S. Rubin Secreted Frizzled-related Protein-1 Binds Directly to Wingless and Is a Biphasic Modulator of Wnt Signaling J. Biol. Chem., February 11, 2000; 275(6): 4374 - 4382. [Abstract] [Full Text] [PDF]

				H. Schumann, J. Holtz, H.-R. Zerkowski, and M. Hatzfeld Expression of secreted frizzled related proteins 3 and 4 in human ventricular myocardium correlates with apoptosis related gene expression Cardiovasc Res, February 1, 2000; 45(3): 720 - 728. [Abstract] [Full Text] [PDF]

				E. D. AUSTIN-WARD and S. C. TAUCHER Familial congenital diaphragmatic hernia: is an imprinting mechanism involved? J. Med. Genet., July 1, 1999; 36(7): 578 - 579. [Full Text]

				S Dennis, M Aikawa, W Szeto, P. d'Amore, and J Papkoff A secreted frizzled related protein, FrzA, selectively associates with Wnt-1 protein and regulates wnt-1 signaling J. Cell Sci., January 11, 1999; 112(21): 3815 - 3820. [Abstract] [PDF]

				Q Xu, P. D'Amore, and S. Sokol Functional and biochemical interactions of Wnts with FrzA, a secreted Wnt antagonist Development, January 12, 1998; 125(23): 4767 - 4776. [Abstract] [PDF]

				H. S. Melkonyan, W. C. Chang, J. P. Shapiro, M. Mahadevappa, P. A. Fitzpatrick, M. C. Kiefer, L. D. Tomei, and S. R. Umansky SARPs: A family of secreted apoptosis-related proteins PNAS, December 9, 1997; 94(25): 13636 - 13641. [Abstract] [Full Text] [PDF]

				K. Lin, S. Wang, M. A. Julius, J. Kitajewski, M. Moos Jr., and F. P. Luyten The cysteine-rich frizzled domain of Frzb-1 is required and sufficient for modulation of Wnt signaling PNAS, October 14, 1997; 94(21): 11196 - 11200. [Abstract] [Full Text] [PDF]

				P. W. Finch, X. He, M. J. Kelley, A. Uren, R. P. Schaudies, N. C. Popescu, S. Rudikoff, S. A. Aaronson, H. E. Varmus, and J. S. Rubin Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action PNAS, June 24, 1997; 94(13): 6770 - 6775. [Abstract] [Full Text] [PDF]

				B. M. McManus and R. D. Gascoyne Pathology and Laboratory Medicine JAMA, June 18, 1997; 277(23): 1887 - 1889. [PDF]

				A. Rattner, J.-C. Hsieh, P. M. Smallwood, D. J. Gilbert, N. G. Copeland, N. A. Jenkins, and J. Nathans A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors PNAS, April 1, 1997; 94(7): 2859 - 2863. [Abstract] [Full Text] [PDF]

				E.M. De Robertis, S. Kim, L. Leyns, S. Piccolo, D. Bachiller, E. Agius, J.A. Belo, A. Yamamoto, A. Hainski-Brousseau, B. Brizuela, et al. Patterning by Genes Expressed in Spemann's Organizer Cold Spring Harb Symp Quant Biol, January 1, 1997; 62(0): 169 - 175. [Abstract] [PDF]