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(Received for publication, July 22, 1996, and in revised form, September 4, 1996)
From the Department of Biochemistry and Molecular Biophysics,
Washington University School of Medicine, St. Louis, Missouri 63110
Caveolae are small, plasma membrane invaginations
that have been implicated in cell signaling. In A431 cells,
approximately half of the total cellular phosphatidylinositol
4,5-bisphosphate (PtdIns 4,5-P2) was found to be localized
in low density, Triton-insoluble membrane domains enriched in caveolin.
Treatment of cells with either epidermal growth factor or bradykinin
for 5 min at 37 °C resulted in approximately a 50% decrease in this
caveolar PtdIns 4,5-P2 with no change in the levels of
plasma membrane PtdIns 4,5-P2. These data suggest that the
PtdIns 4,5-P2 present in cells is largely compartmentalized
and that the caveolar PtdIns 4,5-P2 is subject to
hydrolysis by hormone-stimulated phospholipase C. As growth factor
receptors, seven transmembrane domain receptors, heterotrimeric G
proteins, and the inositol trisphosphate receptor have all been shown
to be enriched in caveolae, these findings suggest that both the
generation and response to inositol trisphosphate is highly
compartmentalized within the cell.
Volume 271, Number 43,
Issue of October 25, 1996
pp. 26453-26456
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
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