| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, June 25, 1996, and in revised form, August 28, 1996)
From the Endocrine Unit and Arthritis Unit, Department of Medicine
and Children's Service, Massachusetts General Hospital and Harvard
Medical School, Boston, Massachusetts 02114
Calcitonin (CT) and parathyroid hormone (PTH),
whose receptors belong to the same family of G protein-coupled
receptors, share no amino acid sequence homology and selectively
activate either CT or PTH receptors. We now show, however, that
reciprocal hybrid ligands (CT/PTH and PTH/CT), which do not activate
the ``wild-type'' receptors, activate PTH/CT and CT/PTH receptor
chimeras, respectively. Our findings indicate that PTH and CT share a
similar architecture with at least two functional, receptor-specific
domains. These domains are sufficiently independent to permit synthetic
hybrid ligands to efficiently activate appropriate receptor chimeras.
Therefore, both ligands follow, despite their very different primary
sequences, a common pattern of ligand-receptor interaction.
Volume 271, Number 43,
Issue of October 25, 1996
pp. 26469-26472
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
COMMUNICATION:
EVIDENCE FOR A COMMON PATTERN OF LIGAND-RECEPTOR
INTERACTION
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Mann, M. J. Wigglesworth, and D. Donnelly Ligand-Receptor Interactions at the Parathyroid Hormone Receptors: Subtype Binding Selectivity Is Mediated via an Interaction between Residue 23 on the Ligand and Residue 41 on the Receptor Mol. Pharmacol., September 1, 2008; 74(3): 605 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Gardella Crystal Structure of the Extracellular Domain of the PTH/PTHrP Receptor IBMS BoneKEy, May 1, 2008; 5(5): 171 - 175. [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Pioszak and H. E. Xu Molecular recognition of parathyroid hormone by its G protein-coupled receptor PNAS, April 1, 2008; 105(13): 5034 - 5039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Parthier, M. Kleinschmidt, P. Neumann, R. Rudolph, S. Manhart, D. Schlenzig, J. Fanghanel, J.-U. Rahfeld, H.-U. Demuth, and M. T. Stubbs Crystal structure of the incretin-bound extracellular domain of a G protein-coupled receptor PNAS, August 28, 2007; 104(35): 13942 - 13947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Kamerzell, S. B. Joshi, D. McClean, L. Peplinskie, K. Toney, D. Papac, M. Li, and C. R. Middaugh Parathyroid hormone is a heparin/polyanion binding protein: Binding energetics and structure modification Protein Sci., June 1, 2007; 16(6): 1193 - 1203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Andreotti, B. L. Mendez, P. Amodeo, M. A. C. Morelli, H. Nakamuta, and A. Motta Structural Determinants of Salmon Calcitonin Bioactivity: THE ROLE OF THE LEU-BASED AMPHIPATHIC {alpha}-HELIX J. Biol. Chem., August 25, 2006; 281(34): 24193 - 24203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Dean, A. Linglart, M. J. Mahon, M. Bastepe, H. Juppner, J. T. Potts Jr., and T. J. Gardella Mechanisms of Ligand Binding to the Parathyroid Hormone (PTH)/PTH-Related Protein Receptor: Selectivity of a Modified PTH(1-15) Radioligand for G{alpha}S-Coupled Receptor Conformations Mol. Endocrinol., April 1, 2006; 20(4): 931 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T Potts Parathyroid hormone: past and present J. Endocrinol., December 1, 2005; 187(3): 311 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pham, M. Dong, J. D. Wade, L. J. Miller, C. J. Morton, H.-l. Ng, M. W. Parker, and P. M. Sexton Insights into Interactions between the {alpha}-Helical Region of the Salmon Calcitonin Antagonists and the Human Calcitonin Receptor using Photoaffinity Labeling J. Biol. Chem., August 5, 2005; 280(31): 28610 - 28622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-R. Barbier, T. J. Gardella, T. Dean, S. MacLean, Z. Potetinova, J. F. Whitfield, and G. E. Willick Backbone-methylated Analogues of the Principle Receptor Binding Region of Human Parathyroid Hormone: EVIDENCE FOR BINDING TO BOTH THE N-TERMINAL EXTRACELLULAR DOMAIN AND EXTRACELLULAR LOOP REGION J. Biol. Chem., June 24, 2005; 280(25): 23771 - 23777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shimizu, T. Dean, J. C. Tsang, A. Khatri, J. T Potts Jr, and T. J. Gardella Novel Parathyroid Hormone (PTH) Antagonists That Bind to the Juxtamembrane Portion of the PTH/PTH-related Protein Receptor J. Biol. Chem., January 21, 2005; 280(3): 1797 - 1807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dong, D. I. Pinon, R. F. Cox, and L. J. Miller Molecular Approximation between a Residue in the Amino-terminal Region of Calcitonin and the Third Extracellular Loop of the Class B G Protein-coupled Calcitonin Receptor J. Biol. Chem., July 23, 2004; 279(30): 31177 - 31182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. Gensure, B. Ponugoti, Y. Gunes, M. R. Papasani, B. Lanske, M. Bastepe, D. A. Rubin, and H. Juppner Identification and Characterization of Two Parathyroid Hormone-Like Molecules in Zebrafish Endocrinology, April 1, 2004; 145(4): 1634 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pham, J. D. Wade, B. W. Purdue, and P. M. Sexton Spatial Proximity between a Photolabile Residue in Position 19 of Salmon Calcitonin and the Amino Terminus of the Human Calcitonin Receptor J. Biol. Chem., February 20, 2004; 279(8): 6720 - 6729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dong, D. I. Pinon, R. F. Cox, and L. J. Miller Importance of the Amino Terminus in Secretin Family G Protein-coupled Receptors: INTRINSIC PHOTOAFFINITY LABELING ESTABLISHES INITIAL DOCKING CONSTRAINTS FOR THE CALCITONIN RECEPTOR J. Biol. Chem., January 9, 2004; 279(2): 1167 - 1175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Gensure, N. Shimizu, J. Tsang, and T. J. Gardella Identification of a Contact Site for Residue 19 of Parathyroid Hormone (PTH) and PTH-Related Protein Analogs in Transmembrane Domain Two of the Type 1 PTH Receptor Mol. Endocrinol., December 1, 2003; 17(12): 2647 - 2658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shimada, X. Chen, T. Cvrk, H. Hilfiker, M. Parfenova, and G. V. Segre Purification and Characterization of a Receptor for Human Parathyroid Hormone and Parathyroid Hormone-related Peptide J. Biol. Chem., August 23, 2002; 277(35): 31774 - 31780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Gensure, P. H. Carter, B. D. Petroni, H. Juppner, and T. J. Gardella Identification of Determinants of Inverse Agonism in a Constitutively Active Parathyroid Hormone/Parathyroid Hormone-related Peptide Receptor by Photoaffinity Cross-linking and Mutational Analysis J. Biol. Chem., November 9, 2001; 276(46): 42692 - 42699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Goold, T. B. Usdin, and S. R. J. Hoare Regions in Rat and Human Parathyroid Hormone (PTH) 2 Receptors Controlling Receptor Interaction with PTH and with Antagonist Ligands J. Pharmacol. Exp. Ther., November 1, 2001; 299(2): 678 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Vilardaga, I. Lin, and R. A. Nissenson Analysis of Parathyroid Hormone (PTH)/Secretin Receptor Chimeras Differentiates the Role of Functional Domains in the PTH/ PTH-Related Peptide (PTHrP) Receptor on Hormone Binding and Receptor Activation Mol. Endocrinol., July 1, 2001; 15(7): 1186 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shimizu, P. H. Carter, A. Khatri, J. T. Potts Jr., and T. J. Gardella Enhanced Activity in Parathyroid Hormone-(1-14) and -(1-11): Novel Peptides for Probing Ligand-Receptor Interactions Endocrinology, July 1, 2001; 142(7): 3068 - 3074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Jonsson, M. R. John, R. C. Gensure, T. J. Gardella, and H. Juppner Tuberoinfundibular Peptide 39 Binds to the Parathyroid Hormone (PTH)/PTH-Related Peptide Receptor, but Functions as an Antagonist Endocrinology, February 1, 2001; 142(2): 704 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Nielsen, L. Z. Nielsen, S. A. Hjorth, M. H. Perrin, and W. W. Vale Constitutive activation of tethered-peptide/ corticotropin-releasing factor receptor chimeras PNAS, August 29, 2000; 97(18): 10277 - 10281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Beyermann, S. Rothemund, N. Heinrich, K. Fechner, J. Furkert, M. Dathe, R. Winter, E. Krause, and M. Bienert A Role for a Helical Connector between Two Receptor Binding Sites of a Long-chain Peptide Hormone J. Biol. Chem., February 25, 2000; 275(8): 5702 - 5709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Behar, A. Bisello, G. Bitan, M. Rosenblatt, and M. Chorev Photoaffinity Cross-linking Identifies Differences in the Interactions of an Agonist and an Antagonist with the Parathyroid Hormone/Parathyroid Hormone-related Protein Receptor J. Biol. Chem., January 7, 2000; 275(1): 9 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Orcel, H. Tajima, Y. Murayama, T. Fujita, S. M. Krane, E. Ogata, S. R. Goldring, and I. Nishimoto Multiple Domains Interacting with Gs in the Porcine Calcitonin Receptor Mol. Endocrinol., January 1, 2000; 14(1): 170 - 182. [Abstract] [Full Text]
|
|
|
|
|
|
P. H. Carter, M. Shimizu, M. D. Luck, and T. J. Gardella The Hydrophobic Residues Phenylalanine 184 and Leucine 187 in the Type-1 Parathyroid Hormone (PTH) Receptor Functionally Interact with the Amino-terminal Portion of PTH-(1-34) J. Biol. Chem., November 5, 1999; 274(45): 31955 - 31960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. H. Carter, H. Jüppner, and T. J. Gardella Studies of the N-Terminal Region of a Parathyroid Hormone-Related Peptide(1-36) Analog: Receptor Subtype-Selective Agonists, Antagonists, and Photochemical Cross-Linking Agents Endocrinology, November 1, 1999; 140(11): 4972 - 4981. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Mannstadt, H. Juppner, and T. J. Gardella Receptors for PTH and PTHrP: their biological importance and functional properties Am J Physiol Renal Physiol, November 1, 1999; 277(5): F665 - F675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. J. Hoare, T. I. Bonner, and T. B. Usdin Comparison of Rat and Human Parathyroid Hormone 2 (PTH2) Receptor Activation: PTH Is a Low Potency Partial Agonist at the Rat PTH2 Receptor Endocrinology, October 1, 1999; 140(10): 4419 - 4425. [Abstract] [Full Text]
|
|
|
|
|
|
B. A. Stillman, M. D. Breyer, and R. M. Breyer Importance of the Extracellular Domain for Prostaglandin EP2 Receptor Function Mol. Pharmacol., September 1, 1999; 56(3): 545 - 551. [Abstract] [Full Text]
|
|
|
|
|
|
S. R. J. Hoare, G. de Vries, and T. B. Usdin Measurement of Agonist and Antagonist Ligand-Binding Parameters at the Human Parathyroid Hormone Type 1 Receptor: Evaluation of Receptor States and Modulation by Guanine Nucleotide J. Pharmacol. Exp. Ther., June 1, 1999; 289(3): 1323 - 1333. [Abstract] [Full Text]
|
|
|
|
|
|
A. E. Adams, A. Bisello, M. Chorev, M. Rosenblatt, and L. J. Suva Arginine 186 in the Extracellular N-Terminal Region of the Human Parathyroid Hormone 1 Receptor Is Essential for Contact with Position 13 of the Hormone Mol. Endocrinol., November 1, 1998; 12(11): 1673 - 1683. [Abstract] [Full Text]
|
|
|
|
|
|
M. Mannstadt, M. D. Luck, T. J. Gardella, and H. Juppner Evidence for a Ligand Interaction Site at the Amino-Terminus of the Parathyroid Hormone (PTH)/PTH-related Protein Receptor from Cross-linking and Mutational Studies J. Biol. Chem., July 3, 1998; 273(27): 16890 - 16896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Turner, S. Mefford, T. Bambino, and R. A. Nissenson Transmembrane Residues Together with the Amino Terminus Limit the Response of the Parathyroid Hormone (PTH) 2 Receptor to PTH-related Peptide J. Biol. Chem., February 13, 1998; 273(7): 3830 - 3837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Clark, T. I. Bonner, A. S. Kim, and T. B. Usdin Multiple Regions of Ligand Discrimination Revealed by Analysis of Chimeric Parathyroid Hormone 2 (PTH2) and PTH/PTH-Related Peptide (PTHrP) Receptors Mol. Endocrinol., February 1, 1998; 12(2): 193 - 206. [Abstract] [Full Text]
|
|
|
|
|
|
C. Bergwitz, S. A. Jusseaume, M. D. Luck, H. Juppner, and T. J. Gardella Residues in the Membrane-spanning and Extracellular Loop Regions of the Parathyroid Hormone (PTH)-2 Receptor Determine Signaling Selectivity for PTH and PTH-related Peptide J. Biol. Chem., November 14, 1997; 272(46): 28861 - 28868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Suva, M. S. Flannery, M. P. Caulfield, D. M. Findlay, H. Jüppner, S. R. Goldring, M. Rosenblatt, and M. Chorev Design, Synthesis and Utility of Novel Benzophenone-Containing Calcitonin Analogs for Photoaffinity Labeling the Calcitonin Receptor J. Pharmacol. Exp. Ther., November 1, 1997; 283(2): 876 - 884. [Abstract] [Full Text]
|
|
|
|
|
|
L. J. Deftos, MD Editorial: There's Something Fishy and Perhaps Even Fowl about the Mammalian Calcitonin Receptor and Its Ligand Endocrinology, February 1, 1997; 138(2): 519 - 520. [Full Text] [PDF]
|
|
|
|
|
|
M. Shimizu, P. H. Carter, and T. J. Gardella Autoactivation of Type-1 Parathyroid Hormone Receptors Containing a Tethered Ligand J. Biol. Chem., June 23, 2000; 275(26): 19456 - 19460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Piserchio, T. Usdin, and D. F. Mierke Structure of Tuberoinfundibular Peptide of 39 Residues J. Biol. Chem., August 25, 2000; 275(35): 27284 - 27290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. J. Hoare, J. A. Clark, and T. B. Usdin Molecular Determinants of Tuberoinfundibular Peptide of 39 Residues (TIP39) Selectivity for the Parathyroid Hormone-2 (PTH2) Receptor. N-TERMINAL TRUNCATION OF TIP39 REVERSES PTH2 RECEPTOR/PTH1 RECEPTOR BINDING SELECTIVITY J. Biol. Chem., August 25, 2000; 275(35): 27274 - 27283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. J. Hoare, T. J. Gardella, and T. B. Usdin Evaluating the Signal Transduction Mechanism of the Parathyroid Hormone 1 Receptor. EFFECT OF RECEPTOR-G-PROTEIN INTERACTION ON THE LIGAND BINDING MECHANISM AND RECEPTOR CONFORMATION J. Biol. Chem., March 9, 2001; 276(11): 7741 - 7753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Gensure, T. J. Gardella, and H. Juppner Multiple Sites of Contact between the Carboxyl-terminal Binding Domain of PTHrP-(1-36) Analogs and the Amino-terminal Extracellular Domain of the PTH/PTHrP Receptor Identified by Photoaffinity Cross-linking J. Biol. Chem., July 27, 2001; 276(31): 28650 - 28658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shimizu, J. Guo, and T. J. Gardella Parathyroid Hormone (PTH)-(1-14) and -(1-11) Analogs Conformationally Constrained by alpha -Aminoisobutyric Acid Mediate Full Agonist Responses via the Juxtamembrane Region of the PTH-1 Receptor J. Biol. Chem., December 21, 2001; 276(52): 49003 - 49012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shimizu, J. T. Potts Jr., and T. J. Gardella Minimization of Parathyroid Hormone. NOVEL AMINO-TERMINAL PARATHYROID HORMONE FRAGMENTS WITH ENHANCED POTENCY IN ACTIVATING THE TYPE-1 PARATHYROID HORMONE RECEPTOR J. Biol. Chem., July 14, 2000; 275(29): 21836 - 21843. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
