| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, June 13, 1996, and in revised form, August 7, 1996)
From the Department of Microbiology and Molecular Genetics, College
of Medicine, University of California, Irvine, California 92697
The leucine-responsive regulatory protein (Lrp)
regulates the expression of many operons in Escherichia coli
including several involved in the metabolism of the
branched-chain amino acids, L-isoleucine,
L-valine, and L-leucine. The ilvGMEDA
operon contains the genes for four of the five enzymes of the
common pathway for the biosynthesis of these amino acids. A high
affinity, consensus-like Lrp-DNA binding site has been identified at an
unusual position in the leader region of this operon 226 base pairs
downstream of the transcriptional initiation site between the
attenuator and the ilvG gene. Binding to this site
facilitates the cooperative binding of a second Lrp protomer to an
adjacent, upstream, secondary site. At higher Lrp concentrations,
binding to a third site is observed. Chemical, enzymatic, and
alkylation protection and interference footprinting experiments
demonstrate that the Lrp homodimer contacts the DNA helix at
symmetrical half-sites present in adjacent major grooves and that the
primary and secondary binding sites are separated by one helical turn
and aligned along the same face of the DNA helix. In vivo,
Lrp represses transcription through the leader-attenuator region
of the ilvGMEDA operon. Lrp-dependent
production of attenuated RNA transcripts is also observed in
vitro. No transcriptional effects are observed, in vivo
or in vitro, in the absence of an intact Lrp primary
binding site. A possible physiological role for Lrp in the regulation
of ilvGMEDA operon expression is discussed.
Volume 271, Number 43,
Issue of October 25, 1996
pp. 26499-26507
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. A. McFarland, S. Lucchini, J. C. D. Hinton, and C. J. Dorman The Leucine-Responsive Regulatory Protein, Lrp, Activates Transcription of the fim Operon in Salmonella enterica Serovar Typhimurium via the fimZ Regulatory Gene J. Bacteriol., January 15, 2008; 190(2): 602 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Park, K. H. Lee, T. Y. Kim, and S. Y. Lee Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation PNAS, May 8, 2007; 104(19): 7797 - 7802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Wagner and M. H. Mulks Identification of the Actinobacillus pleuropneumoniae Leucine-Responsive Regulatory Protein and Its Involvement in the Regulation of In Vivo-Induced Genes Infect. Immun., January 1, 2007; 75(1): 91 - 103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Tuite, K. R. Fraser, and C. P. O'Byrne Homocysteine Toxicity in Escherichia coli Is Caused by a Perturbation of Branched-Chain Amino Acid Biosynthesis J. Bacteriol., July 1, 2005; 187(13): 4362 - 4371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Salmon, S.-p. Hung, K. Mekjian, P. Baldi, G. W. Hatfield, and R. P. Gunsalus Global Gene Expression Profiling in Escherichia coli K12: THE EFFECTS OF OXYGEN AVAILABILITY AND FNR J. Biol. Chem., August 8, 2003; 278(32): 29837 - 29855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-p. Hung, P. Baldi, and G. W. Hatfield Global Gene Expression Profiling in Escherichia coli K12. THE EFFECTS OF LEUCINE-RESPONSIVE REGULATORY PROTEIN J. Biol. Chem., October 18, 2002; 277(43): 40309 - 40323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Fraser, L. Claret, R. Furness, S. Gupta, and C. Hughes Swarming-coupled expression of the Proteus mirabilis hpmBA haemolysin operon Microbiology, July 1, 2002; 148(7): 2191 - 2201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tedin and F. Norel Comparison of {Delta}relA Strains of Escherichia coli and Salmonella enterica Serovar Typhimurium Suggests a Role for ppGpp in Attenuation Regulation of Branched-Chain Amino Acid Biosynthesis J. Bacteriol., November 1, 2001; 183(21): 6184 - 6196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Weyand and D. A. Low Regulation of Pap Phase Variation. Lrp IS SUFFICIENT FOR THE ESTABLISHMENT OF THE PHASE OFF pap DNA METHYLATION PATTERN AND REPRESSION OF pap TRANSCRIPTION IN VITRO J. Biol. Chem., February 4, 2000; 275(5): 3192 - 3200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Madhusudhan, J. Luo, and J. R. Sokatch In Vitro Transcriptional Studies of the bkd Operon of Pseudomonas putida: L-Branched-Chain Amino Acids and D-Leucine Are the Inducers J. Bacteriol., May 1, 1999; 181(9): 2889 - 2894. [Abstract] [Full Text]
|
|
|
|
|
|
A. Napoli, J. van der Oost, C. W. Sensen, R. L. Charlebois, M. Rossi, and M. Ciaramella An Lrp-Like Protein of the Hyperthermophilic Archaeon Sulfolobus solfataricus Which Binds to Its Own Promoter J. Bacteriol., March 1, 1999; 181(5): 1474 - 1480. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Brinkman, I. Dahlke, J. E. Tuininga, T. Lammers, V. Dumay, E. de Heus, J. H. G. Lebbink, M. Thomm, W. M. de Vos, and J. van der Oost An Lrp-like Transcriptional Regulator from the Archaeon Pyrococcus furiosus Is Negatively Autoregulated J. Biol. Chem., December 1, 2000; 275(49): 38160 - 38169. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
