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Volume 271, Number 43, Issue of October 25, 1996 pp. 26543-26546
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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A Potential H-DNA Element in the MUC1 Promoter Does Not Influence Transcription

(Received for publication, May 28, 1996, and in revised form, July 15, 1996)

Gurcharan S. Pahwa , L. James Maher III^§ and Michael A. Hollingsworth

From the  Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198-6805 and the ^§ Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, Minnesota 55905

A purine/pyrimidine mirror repeat element (M-PMR3) in the MUC1 promoter has been shown to form H-DNA under in vitro conditions. We investigated this element for biological function in the regulation of transcription of this gene. Chloramphenicol acetyltransferase reporter-promoter constructs were prepared in which the mirror repeat element (PMR3) was intact, deleted, or modified, and their activities were evaluated by transient transfection assays into the cell lines Capan-2, PANC1, and HT-29. Deletion or modification of M-PMR3 increased expression of chloramphenicol acetyltransferase activity in MUC1-expressing cells; however, a role for an H-DNA structure in this activity was not supported by the results.

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