Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ihn, H.
Right arrow Articles by Trojanowska, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ihn, H.
Right arrow Articles by Trojanowska, M.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 271, Number 43, Issue of October 25, 1996 pp. 26717-26723
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

Transcriptional Regulation of the Human alpha 2(I) Collagen Gene
COMBINED ACTION OF UPSTREAM STIMULATORY AND INHIBITORY CIS-ACTING ELEMENTS

(Received for publication, April 30, 1996, and in revised form, June 25, 1996)

Hironobu Ihn , Kazunori Ohnishi , Takeshi Tamaki , E. Carwile LeRoy and Maria Trojanowska

From the Department of Medicine, Division of Rheumatology, Medical University of South Carolina, Charleston, South Carolina 29425-2229

This study identifies three regions of the human alpha 2(I) collagen promoter involved in the binding of nuclear factors. These regions include sequences from -173 to -155 (footprint I), -133 to -119 (footprint II), and -101 to -72 (footprint III). A novel positive cis-element containing a TCCTCC motif was identified within footprint II. In addition, we demonstrated that a pyrimidine-rich region within footprint I is a binding site for a transcriptional repressor, and a CCAAT motif within footprint III is a binding site for a transcriptional activator. Comparative functional analysis of the cis-acting elements within the proximal 350 base pairs of this promoter, including previously characterized Sp1 binding sites at -300, indicates that constitutive activity of this promoter is regulated equivalently by the three positive cis-acting elements at -300, -125, and -80. Mutations in the repressor site at -160 increase constitutive activity by 4-6-fold. However, simultaneous mutations of the repressor site and the cis-regulatory element at either the -300 or -125 sites result in no increase in constitutive transcription activity suggesting interaction between the activators and repressor elements. In contrast, simultaneous mutation of the CCAAT motif and the repressor site results in about a 4-fold increase, suggesting that activation via the CCAAT motif may be independent of this repressor.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
J. Biol. Chem.Home page
S.-J. Chen, H. Ning, W. Ishida, S. Sodin-Semrl, S. Takagawa, Y. Mori, and J. Varga
The Early-Immediate Gene EGR-1 Is Induced by Transforming Growth Factor-beta and Mediates Stimulation of Collagen Gene Expression
J. Biol. Chem., July 28, 2006; 281(30): 21183 - 21197.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
Y. Asano, H. Ihn, K. Yamane, M. Jinnin, and K. Tamaki
Increased Expression of Integrin {alpha}v{beta}5 Induces the Myofibroblastic Differentiation of Dermal Fibroblasts
Am. J. Pathol., February 1, 2006; 168(2): 499 - 510.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
M. Jinnin, H. Ihn, and K. Tamaki
Characterization of SIS3, a Novel Specific Inhibitor of Smad3, and Its Effect on Transforming Growth Factor-beta1-Induced Extracellular Matrix Expression
Mol. Pharmacol., February 1, 2006; 69(2): 597 - 607.
[Abstract] [Full Text] [PDF]


Home page
Rheumatology (Oxford)Home page
H. Ihn, K. Yamane, Y. Asano, M. Jinnin, and K. Tamaki
Constitutively phosphorylated Smad3 interacts with Sp1 and p300 in scleroderma fibroblasts
Rheumatology, February 1, 2006; 45(2): 157 - 165.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
Y. Asano, H. Ihn, K. Yamane, M. Jinnin, Y. Mimura, and K. Tamaki
Increased Expression of Integrin {alpha}v{beta}3 Contributes to the Establishment of Autocrine TGF-{beta} Signaling in Scleroderma Fibroblasts
J. Immunol., December 1, 2005; 175(11): 7708 - 7718.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
E. W. Pirok III, M. S. Domowicz, J. Henry, Y. Wang, M. Santore, M. M. Mueller, and N. B. Schwartz
APBP-1, a DNA/RNA-binding Protein, Interacts with the Chick Aggrecan Regulatory Region
J. Biol. Chem., October 21, 2005; 280(42): 35606 - 35616.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
M. Jinnin, H. Ihn, Y. Mimura, Y. Asano, K. Yamane, and K. Tamaki
Matrix metalloproteinase-1 up-regulation by hepatocyte growth factor in human dermal fibroblasts via ERK signaling pathway involves Ets1 and Fli1
Nucleic Acids Res., June 21, 2005; 33(11): 3540 - 3549.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
Y. Mimura, H. Ihn, M. Jinnin, Y. Asano, K. Yamane, and K. Tamaki
Constitutive Thrombospondin-1 Overexpression Contributes to Autocrine Transforming Growth Factor-{beta} Signaling in Cultured Scleroderma Fibroblasts
Am. J. Pathol., May 1, 2005; 166(5): 1451 - 1463.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
M. Jinnin, H. Ihn, K. Yamane, Y. Mimura, Y. Asano, and K. Tamaki
{alpha}2(I) collagen gene regulation by protein kinase C signaling in human dermal fibroblasts
Nucleic Acids Res., March 1, 2005; 33(4): 1337 - 1351.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Tanaka, T. T. Antoniv, K. Liu, L. Wang, D. J. Wells, F. Ramirez, and G. Bou-Gharios
Cooperativity between Far Upstream Enhancer and Proximal Promoter Elements of the Human {alpha}2(I) Collagen (COL1A2) Gene Instructs Tissue Specificity in Transgenic Mice
J. Biol. Chem., December 31, 2004; 279(53): 56024 - 56031.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Yamanaka, D. Shegogue, H. Pei, S. Bu, A. Bielawska, J. Bielawski, B. Pettus, Y. A. Hannun, L. Obeid, and M. Trojanowska
Sphingosine Kinase 1 (SPHK1) Is Induced by Transforming Growth Factor-{beta} and Mediates TIMP-1 Up-regulation
J. Biol. Chem., December 24, 2004; 279(52): 53994 - 54001.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
H. Nagato, N. Matsuo, H. Sumiyoshi, K. Sakata-Takatani, M. Nasu, and H. Yoshioka
The Transcription Factor CCAAT-binding Factor CBF/NF-Y and Two Repressors Regulate the Core Promoter of the Human Pro-{alpha}3(V) Collagen Gene (COL5A3)
J. Biol. Chem., November 5, 2004; 279(45): 46373 - 46383.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Jinnin, H. Ihn, K. Yamane, and K. Tamaki
Interleukin-13 Stimulates the Transcription of the Human {alpha}2(I) Collagen Gene in Human Dermal Fibroblasts
J. Biol. Chem., October 1, 2004; 279(40): 41783 - 41791.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
Y. Asano, H. Ihn, K. Yamane, M. Jinnin, Y. Mimura, and K. Tamaki
Phosphatidylinositol 3-Kinase Is Involved in {alpha}2(I) Collagen Gene Expression in Normal and Scleroderma Fibroblasts
J. Immunol., June 1, 2004; 172(11): 7123 - 7135.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
Y. Asano, H. Ihn, K. Yamane, M. Kubo, and K. Tamaki
Increased Expression Levels of Integrin {alpha}v{beta}5 on Scleroderma Fibroblasts
Am. J. Pathol., April 1, 2004; 164(4): 1275 - 1292.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
K. Yamane, H. Ihn, Y. Asano, M. Jinnin, and K. Tamaki
Antagonistic Effects of TNF-{alpha} on TGF-{beta} Signaling Through Down-Regulation of TGF-{beta} Receptor Type II in Human Dermal Fibroblasts
J. Immunol., October 1, 2003; 171(7): 3855 - 3862.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
M. Kubo, J. Czuwara-Ladykowska, O. Moussa, M. Markiewicz, E. Smith, R. M. Silver, S. Jablonska, M. Blaszczyk, D. K. Watson, and M. Trojanowska
Persistent Down-Regulation of Fli1, a Suppressor of Collagen Transcription, in Fibrotic Scleroderma Skin
Am. J. Pathol., August 1, 2003; 163(2): 571 - 581.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Sato, M. Markiewicz, M. Yamanaka, A. Bielawska, C. Mao, L. M. Obeid, Y. A. Hannun, and M. Trojanowska
Modulation of Transforming Growth Factor-beta (TGF-beta ) Signaling by Endogenous Sphingolipid Mediators
J. Biol. Chem., March 7, 2003; 278(11): 9276 - 9282.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
K. Higashi, Y. Inagaki, N. Suzuki, S. Mitsui, A. Mauviel, H. Kaneko, and I. Nakatsuka
Y-box-binding Protein YB-1 Mediates Transcriptional Repression of Human alpha 2(I) Collagen Gene Expression by Interferon-gamma
J. Biol. Chem., February 7, 2003; 278(7): 5156 - 5162.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Gaidarova and S. A. Jimenez
Inhibition of Basal and Transforming Growth Factor-beta -induced Stimulation of COL1A1 Transcription by the DNA Intercalators, Mitoxantrone and WP631, in Cultured Human Dermal Fibroblasts
J. Biol. Chem., October 4, 2002; 277(41): 38737 - 38745.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
P. K. Sengupta, J. Fargo, and B. D. Smith
The RFX Family Interacts at the Collagen (COL1A2) Start Site and Represses Transcription
J. Biol. Chem., July 5, 2002; 277(28): 24926 - 24937.
[Abstract] [Full Text] [PDF]


Home page
Exp. Biol. Med.Home page
A. K. Ghosh
Factors Involved in the Regulation of Type I Collagen Gene Expression: Implication in Fibrosis
Experimental Biology and Medicine, May 1, 2002; 227(5): 301 - 314.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
H. Ihn, K. Yamane, Y. Asano, M. Kubo, and K. Tamaki
IL-4 Up-Regulates the Expression of Tissue Inhibitor of Metalloproteinase-2 in Dermal Fibroblasts Via the p38 Mitogen-Activated Protein Kinase-Dependent Pathway
J. Immunol., February 15, 2002; 168(4): 1895 - 1902.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
S. Uchida, Y. Tanaka, H. Ito, F. Saitoh-Ohara, J. Inazawa, K. K. Yokoyama, S. Sasaki, and F. Marumo
Transcriptional Regulation of the CLC-K1 Promoter by myc-Associated Zinc Finger Protein and Kidney-Enriched Kruppel-Like Factor, a Novel Zinc Finger Repressor
Mol. Cell. Biol., October 1, 2000; 20(19): 7319 - 7331.
[Abstract] [Full Text]


Home page
J. Immunol.Home page
H. Ihn and K. Tamaki
Oncostatin M Stimulates the Growth of Dermal Fibroblasts Via a Mitogen-Activated Protein Kinase-Dependent Pathway
J. Immunol., August 15, 2000; 165(4): 2149 - 2155.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
D. J. Kouba, K.-Y. Chung, T. Nishiyama, L. Vindevoghel, A. Kon, J. F. Klement, J. Uitto, and A. Mauviel
Nuclear Factor-{kappa}B Mediates TNF-{alpha} Inhibitory Effect on {alpha}2(I) Collagen (COL1A2) Gene Transcription in Human Dermal Fibroblasts
J. Immunol., April 1, 1999; 162(7): 4226 - 4234.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
H. Ihn, E. C. LeRoy, and M. Trojanowska
Oncostatin M Stimulates Transcription of the Human alpha 2(I) Collagen Gene via the Sp1/Sp3-binding Site
J. Biol. Chem., September 26, 1997; 272(39): 24666 - 24672.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
P. Greenwel, Y. Inagaki, W. Hu, M. Walsh, and F. Ramirez
Sp1 Is Required for the Early Response of alpha 2(I) Collagen to Transforming Growth Factor-beta 1
J. Biol. Chem., August 8, 1997; 272(32): 19738 - 19745.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A.-C. Poncelet and H. W. Schnaper
Sp1 and Smad Proteins Cooperate to Mediate Transforming Growth Factor-beta 1-induced alpha 2(I) Collagen Expression in Human Glomerular Mesangial Cells
J. Biol. Chem., March 2, 2001; 276(10): 6983 - 6992.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
E. W. Pirok III, J. Henry, and N. B. Schwartz
cis Elements That Control the Expression of Chick Aggrecan
J. Biol. Chem., May 11, 2001; 276(20): 16894 - 16903.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. Czuwara-Ladykowska, F. Shirasaki, P. Jackers, D. K. Watson, and M. Trojanowska
Fli-1 Inhibits Collagen Type I Production in Dermal Fibroblasts via an Sp1-dependent Pathway
J. Biol. Chem., June 8, 2001; 276(24): 20839 - 20848.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
Y. Inagaki, T. Nemoto, A. Nakao, P. t. Dijke, K. Kobayashi, K. Takehara, and P. Greenwel
Interaction between GC Box Binding Factors and Smad Proteins Modulates Cell Lineage-specific alpha 2(I) Collagen Gene Transcription
J. Biol. Chem., May 4, 2001; 276(19): 16573 - 16579.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement