HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cippitelli, M. Articles by Young, H. A. Search for Related Content PubMed PubMed Citation Articles by Cippitelli, M. Articles by Young, H. A. Social Bookmarking                      What's this?

Volume 271, Number 43, Issue of October 25, 1996 pp. 26783-26793
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Retinoic Acid-induced Transcriptional Modulation of the Human Interferon- Promoter

(Received for publication, July 29, 1996)

Marco Cippitelli , Jianping Ye ^§ , Vincenzo Viggiano ^§ , Antonio Sica ^§ , Paritosh Ghosh ^§ , Alberto Gulino ^¶ , Angela Santoni ^'' and Howard A. Young ^§

From the  Intramural Research Support Program, Scientific Application International Corporation Frederick, and the ^§ Laboratory of Experimental Immunology, Division of Basic Science, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, the ^¶ Department of Experimental Medicine, University of L'Aquila, 67100 L'Aquila, Italy, the  Department of Experimental Medicine and Pathology, University ``La Sapienza,'' Roma 00158, Italy, and the ^'' Laboratory of Pathophysiology, Regina Elena Cancer Institute, Roma 00158, Italy

Disregulation of vitamin A metabolism is able to generate different immunological effects, including altered response to infection, reduced IgG production, and differential regulation of cytokine gene expression (including interleukin-2 and -4 and interferon- (IFN-)). In particular, IFN- gene expression is significantly affected by vitamin A and/or its derivatives (e.g. retinoic acid (RA)). Here, we analyze the effect of retinoic acid on IFN- transcription. Transient transfection assays in the human T lymphoblastoid cell line Jurkat demonstrated that the activation of the IFN- promoter was significantly down-regulated in the presence of RA. Surprisingly, two different AP-1/CREB-ATF-binding elements situated in the initial 108 base pairs of the IFN- promoter and previously shown to be critical for transcriptional activity were unaffected by RA. Utilizing promoter deletions and electrophoretic mobility shift analysis, we identified a USF/EGR-1-binding element cooperating in the modulation of IFN- promoter activity by RA. This element was found to be situated in a position of the IFN- promoter close to a silencer element previously identified in our laboratory. These results suggest that direct modulation of IFN- promoter activity is one of the possible mechanisms involved in the inhibitory effect of retinoids on IFN- gene expression.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Yamauchi, A. Ishibashi, K. Shikata, N. Tokuhara, K.-i. Seino, S. Kobayashi, and M. Nagai Effect of E6060 [4-{5-[7-Fluoro-4-(trifluoromethyl)benzo[b]furan-2-yl]-1H-2-pyrrolyl}benzoic Acid], a Novel Subtype-Selective Retinoid, on Lupus-Like Nephritis in Female (NZBxNZW)F1 Mice J. Pharmacol. Exp. Ther., March 1, 2005; 312(3): 938 - 944. [Abstract] [Full Text] [PDF]

				K.-i. Seino, T. Yamauchi, K. Shikata, S. Kobayashi, M. Nagai, M. Taniguchi, and K. Fukao Prevention of acute and chronic allograft rejection by a novel retinoic acid receptor-{alpha}-selective agonist Int. Immunol., May 1, 2004; 16(5): 665 - 673. [Abstract] [Full Text] [PDF]

				K. Kinoshita, B.-S. Yoo, Y. Nozaki, M. Sugiyama, S. Ikoma, M. Ohno, M. Funauchi, and A. Kanamaru Retinoic Acid Reduces Autoimmune Renal Injury and Increases Survival in NZB/W F1 Mice J. Immunol., June 1, 2003; 170(11): 5793 - 5798. [Abstract] [Full Text] [PDF]

				M. Cippitelli, C. Fionda, D. Di Bona, A. Lupo, M. Piccoli, L. Frati, and A. Santoni The Cyclopentenone-Type Prostaglandin 15-Deoxy-{Delta}12,14-Prostaglandin J2 Inhibits CD95 Ligand Gene Expression in T Lymphocytes: Interference with Promoter Activation Via Peroxisome Proliferator-Activated Receptor-{gamma}-Independent Mechanisms J. Immunol., May 1, 2003; 170(9): 4578 - 4592. [Abstract] [Full Text] [PDF]

				C. B. Stephensen, R. Rasooly, X. Jiang, M. A. Ceddia, C. T. Weaver, R. A. S. Chandraratna, and R. P. Bucy Vitamin A Enhances in Vitro Th2 Development Via Retinoid X Receptor Pathway J. Immunol., May 1, 2002; 168(9): 4495 - 4503. [Abstract] [Full Text] [PDF]

				Y. Takeba, H. Nagafuchi, M. Takeno, J.-i. Kashiwakura, and N. Suzuki Txk, a Member of Nonreceptor Tyrosine Kinase of Tec Family, Acts as a Th1 Cell-Specific Transcription Factor and Regulates IFN-{gamma} Gene Transcription J. Immunol., March 1, 2002; 168(5): 2365 - 2370. [Abstract] [Full Text] [PDF]

				M. Cippitelli, C. Fionda, D. Di Bona, F. Di Rosa, A. Lupo, M. Piccoli, L. Frati, and A. Santoni Negative Regulation of CD95 Ligand Gene Expression by Vitamin D3 in T Lymphocytes J. Immunol., February 1, 2002; 168(3): 1154 - 1166. [Abstract] [Full Text] [PDF]

				D. M. Shin, F. R. Khuri, B. Murphy, A. S. Garden, G. Clayman, M. Francisco, D. Liu, B. S. Glisson, L. Ginsberg, V. Papadimitrakopoulou, et al. Combined Interferon-Alfa, 13-cis-Retinoic Acid, and Alpha-Tocopherol in Locally Advanced Head and Neck Squamous Cell Carcinoma: Novel Bioadjuvant Phase II Trial J. Clin. Oncol., June 15, 2001; 19(12): 3010 - 3017. [Abstract] [Full Text] [PDF]

				Y. Devaux, S. Grosjean, C. Seguin, C. David, B. Dousset, F. Zannad, C. Meistelman, N. De Talance, P.-M. Mertes, and D. Ungureanu-Longrois Retinoic acid and host-pathogen interactions: effects on inducible nitric oxide synthase in vivo Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1045 - E1053. [Abstract] [Full Text] [PDF]

				P. Neuville, M.-L. Bochaton-Piallat, and G. Gabbiani Retinoids and Arterial Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., August 1, 2000; 20(8): 1882 - 1888. [Full Text] [PDF]

				D. Cui, Z. Moldoveanu, and C. B. Stephensen High-Level Dietary Vitamin A Enhances T-Helper Type 2 Cytokine Production and Secretory Immunoglobulin A Response to Influenza A Virus Infection in BALB/c Mice J. Nutr., May 1, 2000; 130(5): 1132 - 1139. [Abstract] [Full Text]

				R. Gonsky, R. L. Deem, J. H. Bream, D. H. Lee, H. A. Young, and S. R. Targan Mucosa-Specific Targets for Regulation of IFN-{gamma} Expression: Lamina Propria T Cells Use Different cis-Elements than Peripheral Blood T Cells to Regulate Transactivation of IFN-{gamma} Expression J. Immunol., February 1, 2000; 164(3): 1399 - 1407. [Abstract] [Full Text] [PDF]

				H. Kojima, Y. Aizawa, Y. Yanai, K. Nagaoka, M. Takeuchi, T. Ohta, H. Ikegami, M. Ikeda, and M. Kurimoto An Essential Role for NF-{kappa}B in IL-18-Induced IFN-{gamma} Expression in KG-1 Cells J. Immunol., May 1, 1999; 162(9): 5063 - 5069. [Abstract] [Full Text] [PDF]