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Volume 271, Number 43, Issue of October 25, 1996 pp. 26908-26913
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Molecular Cloning and Cold-inducible Gene Expression of Ferritin H Subunit Isoforms in Rainbow Trout Cells

(Received for publication, December 6, 1995, and in revised form, June 6, 1996)

From the National Research Institute of Fisheries Science, Fukuura, Kanazawa, Yokohama 236, Japan

Cold acclimation of rainbow trout cells is considered to be mediated by alterations in the mRNAs and proteins present in cold-treated cells. A subtracted cDNA library from cold-treated rainbow trout RTG-2 cells was constructed and screened to isolate cDNA induced in the cold-treated cells in order to elucidate which genes are induced by cold acclimation. A set of cDNA clones encoding three members of ferritin H isoforms was isolated as cold-inducible genes. Northern blot analysis and nuclear run-on transcription assay showed that the transcription and accumulation of the ferritin H isoforms mRNA were enhanced by cold acclimation. Furthermore, the ferritin level in the trout cells increased on cold acclimation in response to a temperature shift from 22 °C to 4 °C. When the trout cells were subjected to 4 °C under the condition of a decreased ferritin H level obtained by the addition of an antisense oligonucleotide, cell growth was apparently inhibited. These findings indicate an association between the induction of ferritin H and cellular mechanisms during cold acclimation of trout cells.

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