HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nornes, S. Articles by Johansen, T. Search for Related Content PubMed PubMed Citation Articles by Nornes, S. Articles by Johansen, T. Social Bookmarking                      What's this?

Volume 271, Number 43, Issue of October 25, 1996 pp. 26914-26923
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Zebrafish Pax9 Encodes Two Proteins with Distinct C-terminal Transactivating Domains of Different Potency Negatively Regulated by Adjacent N-terminal Sequences

(Received for publication, November 28, 1995, and in revised form, July 3, 1996)

Svanhild Nornes , Ingvild Mikkola , Stefan Krauss , Madjid Delghandi , Maria Perander and Terje Johansen

From the  Departments of Biochemistry and  Molecular Genetics, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway

We describe the isolation of cDNA clones for zebrafish Pax9. Pax9 expression was initiated at the end of the segmentation period in mesenchymal sclerotome cells on both sides of the notochord similarly to the corresponding mouse and chick genes. Two transcripts, Pax9a and -b, are generated by alternative splicing. The gene contains 4 exons with exon 3 being included in the Pax9a transcript and spliced out in the Pax9b transcript. The Pax9a and -b proteins are identical for 212 amino acids from the N terminus but contain distinct C-terminal regions of 131 and 58 amino acids, respectively. The paired domain of Pax9 displayed a binding-site specificity distinct from Pax6 but similar to Pax1 and -2. Both Pax9a and -b activated a promoter containing a paired domain binding site. However, this activation was observed when low amounts of Pax9 expression vectors were used. Higher amounts led to a sharp decrease in the activation and even turned into repression. Both the distinct C-terminal regions of Pax9a and -b harbored transcriptional activating domains of different potency not revealed in the context of the full-length proteins due to a negative influence of the N-terminal region including the paired domain.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Zhang An evo-devo view on the origin of the backbone: evolutionary development of the vertebrae Integr. Comp. Biol., July 8, 2009; (2009) icp061v1. [Abstract] [Full Text] [PDF]

				K. Laue, S. Daujat, J. G. Crump, N. Plaster, H. H. Roehl, Tubingen 2000 Screen Consortium, C. B. Kimmel, R. Schneider, and M. Hammerschmidt The multidomain protein Brpf1 binds histones and is required for Hox gene expression and segmental identity Development, June 1, 2008; 135(11): 1935 - 1946. [Abstract] [Full Text] [PDF]

				E. Dumont, C. Ralliere, and P.-Y. Rescan Identification of novel genes including Dermo-1, a marker of dermal differentiation, expressed in trout somitic external cells J. Exp. Biol., April 1, 2008; 211(7): 1163 - 1168. [Abstract] [Full Text] [PDF]

				M. Nowak and M. Hammerschmidt Ubc9 Regulates Mitosis and Cell Survival during Zebrafish Development Mol. Biol. Cell, December 1, 2006; 17(12): 5324 - 5336. [Abstract] [Full Text] [PDF]

				T. V. Pereira, F. M. Salzano, A. Mostowska, W. H. Trzeciak, A. Ruiz-Linares, J. A. B. Chies, C. Saavedra, C. Nagamachi, A. M. Hurtado, K. Hill, et al. Natural selection and molecular evolution in primate PAX9 gene, a major determinant of tooth development PNAS, April 11, 2006; 103(15): 5676 - 5681. [Abstract] [Full Text] [PDF]

				J. Pietsch, J.-M. Delalande, B. Jakaitis, J. D. Stensby, S. Dohle, W. S. Talbot, D. W. Raible, and I. T. Shepherd lessen encodes a zebrafish trap100 required for enteric nervous system development Development, February 1, 2006; 133(3): 395 - 406. [Abstract] [Full Text] [PDF]

				M. D. Mackereth, S.-J. Kwak, A. Fritz, and B. B. Riley Zebrafish pax8 is required for otic placode induction and plays a redundant role with Pax2 genes in the maintenance of the otic placode Development, January 15, 2005; 132(2): 371 - 382. [Abstract] [Full Text] [PDF]

				M. Okabe and A. Graham The origin of the parathyroid gland PNAS, December 21, 2004; 101(51): 17716 - 17719. [Abstract] [Full Text] [PDF]

				A. Fleming, R. Keynes, and D. Tannahill A central role for the notochord in vertebral patterning Development, February 15, 2004; 131(4): 873 - 880. [Abstract] [Full Text] [PDF]

				J. K. Mensah, T. Ogawa, H. Kapadia, A. C. Cavender, and R. N. D'Souza Functional Analysis of a Mutation in PAX9 Associated with Familial Tooth Agenesis in Humans J. Biol. Chem., February 13, 2004; 279(7): 5924 - 5933. [Abstract] [Full Text] [PDF]

				K. Tan, A. L. Shaw, B. Madsen, K. Jensen, J. Taylor-Papadimitriou, and P. S. Freemont Human PLU-1 Has Transcriptional Repression Properties and Interacts with the Developmental Transcription Factors BF-1 and PAX9 J. Biol. Chem., May 30, 2003; 278(23): 20507 - 20513. [Abstract] [Full Text] [PDF]

				I. Rodrigo, R. E. Hill, R. Balling, A. Munsterberg, and K. Imai Pax1 and Pax9 activate Bapx1 to induce chondrogenic differentiation in the sclerotome Development, February 1, 2003; 130(3): 473 - 482. [Abstract] [Full Text] [PDF]

				D. J. Swanson, M. Adachi, and E. J. Lewis The Homeodomain Protein Arix Promotes Protein Kinase A-dependent Activation of the Dopamine beta -Hydroxylase Promoter through Multiple Elements and Interaction with the Coactivator cAMP-response Element-binding Protein-binding Protein J. Biol. Chem., January 28, 2000; 275(4): 2911 - 2923. [Abstract] [Full Text] [PDF]

				N. S. Belaguli, W. Zhou, T.-H. T. Trinh, M. W. Majesky, and R. J. Schwartz Dominant Negative Murine Serum Response Factor: Alternative Splicing within the Activation Domain Inhibits Transactivation of Serum Response Factor Binding Targets Mol. Cell. Biol., July 1, 1999; 19(7): 4582 - 4591. [Abstract] [Full Text] [PDF]

				I. Mikkola, J.-A. Bruun, G. Bjorkoy, T. Holm, and T. Johansen Phosphorylation of the Transactivation Domain of Pax6 by Extracellular Signal-regulated Kinase and p38 Mitogen-activated Protein Kinase J. Biol. Chem., May 21, 1999; 274(21): 15115 - 15126. [Abstract] [Full Text] [PDF]

				C Takke and J. Campos-Ortega her1, a zebrafish pair-rule like gene, acts downstream of notch signalling to control somite development Development, January 7, 1999; 126(13): 3005 - 3014. [Abstract] [PDF]

				M Ogasawara, H Wada, H Peters, and N Satoh Developmental expression of Pax1/9 genes in urochordate and hemichordate gills: insight into function and evolution of the pharyngeal epithelium Development, January 6, 1999; 126(11): 2539 - 2550. [Abstract] [PDF]