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(Received for publication, February 21, 1996, and in revised form, July 26, 1996)
From the Expression of
Volume 271, Number 43,
Issue of October 25, 1996
pp. 26998-27004
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
-Amylase Genes That Is Modulated through Complicated
Transcriptional and Posttranscriptional Processes
§
,¶
,
Institute of Molecular Biology,
-amylase genes in cultured rice
suspension cells is induced by sucrose starvation. To study the
mechanism of sugar metabolite regulation on the expression of
individual -amylase genes, DNA fragments specific to each of eight
rice -amylase genes were synthesized and used as gene-specific
probes. Comparison of the relative abundance of mRNA revealed that
expression of the eight -amylase genes in rice cells was
differentially regulated by sucrose starvation. Accumulation of all the
-amylase mRNAs increased in response to sucrose starvation;
however, levels of the Amy3 and Amy8
mRNAs were distinctly higher and constituted 90% of total
-amylase mRNAs. RNA gel blot and nuclear run-on transcription
analyses demonstrated a positive correlation between the
increased transcription rates and the elevated steady-state levels of
-amylase mRNAs induced by sucrose starvation. The half-lives of
Amy3, Amy7, and Amy8 were
prolonged by sucrose-starvation; however, the stability of the three
mRNAs seems controlled by different mechanisms. The translation
inhibitors cycloheximide and anisomycin preferentially blocked the
sucrose-suppressed expression of Amy3 but not that of
Amy7 and Amy8. These inhibitors also
enhanced the sucrose starvation-induced accumulation of
Amy3 mRNA but not that of Amy7 or
Amy8 mRNAs. Cycloheximide did not significantly
alter the transcription rates of -amylase genes, suggesting that
labile proteins may selectively stabilize the Amy7
and Amy8 mRNAs but destabilize the
Amy3 mRNA.
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