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ENDS
(Received for publication, June 6, 1996)
From the Department of Molecular Biology and Microbiology, Tufts
University School of Medicine, Boston, Massachusetts 02111-1800
The cleavage/polyadenylation factor I (CF I) is
one of four factors required for mRNA 3
end formation in the yeast
Saccharomyces cerevisiae. Here we describe the purification
of CF I and its separation into two components, CF IA and CF IB. Both
components are needed to reconstitute CF I activity in cleavage and
poly(A) addition. CF IA consists of a complex of four polypeptides of
76, 70, 50, and 38 kDa, and CF IB is a single 73-kDa polypeptide. The
76- and 38-kDa subunits of CF IA correspond to the previously
identified RNA14 and RNA15 proteins. The RNA14 protein, but not the 70- or 50-kDa proteins, coimmunoprecipitates with the RNA15 protein,
indicating that RNA14 and RNA15 proteins exist in a tight complex.
RNA15 is the only subunit of CF I that can be cross-linked to
pre-mRNA.
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